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16 Difference Between Brightfield and Fluorescence Microscope.

MN Editors

In My previous notes i have already discussed about Brightfield Microscope and Fluorescence Microscope, their working principle, parts, definition, application, advantages, disadvantages and light path. You can check them out.

Difference Between Brightfield Microscope and Fluorescence Microscope

Difference Between Brightfield Microscope and Fluorescence Microscope.

Topic Brightfield Microscope Fluorescence Microscope
Invented by Antonie van Leeuwenhoek (1632–1724) Antonie van Leeuwenhoek (1632–1724)
Fluorochromes Not required Required.
Specimen Image Form dark image against bright background. Form bright image against dark background.
Light Source Light source may be natural or artificial. Light source must be artificial.
Exciter Filter Not required. Required.
Dichroic Filter Not Required. Required.
Emission filter Absent Present
Staining The specimen staining is done with the safranin, crystal violet, etc. The specimen staining is done with fluorochromes or Fluorescent dey.
Specimen Preparation Quick and simple processes. Long and complex processes.
Expensive It is cheaper. Expensive and Specimen preparation is a costly process.
Image Detector Absent Present
Image Visualization Image is visualize through the eyepiece. Image is visualize on a digital display.
Protein Localization It can not localize specific proteins within the cell. It can localize specific proteins within the cell.
multicolor staining Not allowed Allowed
Living cell study Not possible Possible
fluorescence and phosphorescence Absent Present

Brightfield Microscope Image

Bright Field Microscope

Fluorescence Microscope Image

Fluorescence Microscope Image


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