Differences

16 Difference Between Brightfield and Fluorescence Microscope.

Table of Contents show 1 Difference Between Brightfield Microscope and Fluorescence Microscope. 2 Reference 3 Don't miss a post In My previous...

MN Editors avatar
MN Editors
This article writter by MN Editors on July 21, 2020

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

· 40 sec read >
Difference Between Brightfield Microscope and Fluorescence Microscope

In My previous notes i have already discussed about Brightfield Microscope and Fluorescence Microscope, their working principle, parts, definition, application, advantages, disadvantages and light path. You can check them out.

Difference Between Brightfield Microscope and Fluorescence Microscope

Difference Between Brightfield Microscope and Fluorescence Microscope.

Topic Brightfield MicroscopeFluorescence Microscope
Invented byAntonie van Leeuwenhoek (1632–1724)Antonie van Leeuwenhoek (1632–1724)
FluorochromesNot requiredRequired.
Specimen Image Form dark image against bright background.Form bright image against dark background.
Light SourceLight source may be natural or artificial.Light source must be artificial.
Exciter FilterNot required.Required.
Dichroic FilterNot Required.Required.
Emission filterAbsentPresent
Staining The specimen staining is done with the safranin, crystal violet, etc.The specimen staining is done with fluorochromes or Fluorescent dey.
Specimen PreparationQuick and simple processes.Long and complex processes.
ExpensiveIt is cheaper.Expensive and Specimen preparation is a costly process.
Image DetectorAbsentPresent
Image VisualizationImage is visualize through the eyepiece.Image is visualize on a digital display.
Protein LocalizationIt can not localize specific proteins within the cell.It can localize specific proteins within the cell.
multicolor stainingNot allowedAllowed
Living cell studyNot possiblePossible
fluorescence and phosphorescenceAbsentPresent

Brightfield Microscope Image

Bright Field Microscope

Fluorescence Microscope Image

Fluorescence Microscope Image

Reference

close

Don't miss a post

Sign up to receive latest post notification

We don’t spam! Read our privacy policy for more info.

microbiology note app
microbiology note app qr code Scane to download
Download Microbiology Note App Download this app for free from google play store and read ads free notes
Need a Note? Request us
Request

Leave a Reply

Your email address will not be published.

2
0

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

Join the Newsletter

Subscribe to our weekly newsletter below and never miss the latest articles.

Ads Blocker Image Powered by Code Help Pro

Ads Blocker Detected!!!

We have detected that you are using extensions to block ads. Please support us by disabling these ads blocker.

Powered By
CHP Adblock Detector Plugin | Codehelppro