Table of Contents
1. Gene Cloning and DNA Analysis: An Introduction
Author: T. A. Brown
Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.
Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like:
- The strategies used by researchers and industry practitioners to assemble genome sequences
- Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes
- Includes the use and application of gene editing strategies
- Interbreeding between Neanderthals and Homo Sapiens
Gene Cloning and DNA Analysis: An Introduction, 8th Editionis an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.
2. Biotechnology: Applying the Genetic Revolution
Author: David P. Clark (Author), Nanette J. Pazdernik (Author)
Unlike most biotechnology textbooks, Dr. David P. Clark’s Biotechnology approaches modern biotechnology from a molecular basis, which grew out of the increasing biochemical understanding of physiology. Using straightforward, less-technical jargon, Clark manages to introduce each chapter with a basic concept that ultimately evolves into a more specific detailed principle. This up-to-date text covers a wide realm of topics, including forensics and bioethics, using colorful illustrations and concise applications.
This book will help readers understand molecular biotechnology as a scientific discipline, how the research in this area is conducted, and how this technology may impact the future.
· Up-to-date text focuses on modern biotechnology with a molecular foundation
· Basic concepts followed by more detailed, specific applications
· Clear, color illustrations of key topics and concepts
· Clearly written without overly technical jargon or complicated examples
3. Principles of Gene Manipulation and Genomics
Author: Sandy B. Primrose (Author), Richard Twyman (Author)
The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics.
- Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics
- Includes two new chapters on the applications of genomics
- An accompanying website – www.blackwellpublishing.com/primrose – provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format.
- An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.
4. Molecular Biology Techniques: A Classroom Laboratory Manual
Author: Heather B. Miller (Author), D. Scott Witherow (Author), Sue Carson (Author)
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project” approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein – students can actually visualize positive clones following IPTG induction.
- Cover basic concepts and techniques used in molecular biology research labs
- Student-tested labs proven successful in a real classroom laboratories
- Exercises simulate a cloning project that would be performed in a real research lab
- “Project” approach to experiments gives students an overview of the entire process
- Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
5. Molecular Cloning: A Laboratory Manual
Author: Michael R. Green (Author), Joseph Sambrook (Author)
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Please take a look at our book preview site at molecularcloning.com. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard–the one indispensable molecular biology laboratory manual and reference source.
6. Prescott, harley and klein: MICROBIOLOGY
Author: Joanne M. Willey (Author), Linda Sherwood (Author), Christopher woolverton (Author)
This is 7th edition of the prescott’s microbiology providing a balance, comprehensive introduction to all major areas of micrrbiology.
7. Genomes 3
Author: Brown (Author), T. A. (Author)
Covering molecular genetics from the basics through to genome expression and molecular phylogenetics, Genomes 3 is the latest edition of this pioneering textbook. Newly updated to incorporate the recent major advances, Genomes 3 is an invaluable companion for any undergraduate throughout their studies in molecular genetics. Following extensive reviewing, the new edition has been significantly restructured. The single chapter on genome anatomies has been expanded into three chapters to incorporate the latest sequencing achievements. An additional chapter on understanding genome expression has also been included, while the chapters on studying genomes have been brought to the front of the book to align it more closely to the practical reality of molecular genetics tuition. The end-of-chapter exercises have been overhauled and extended to give students and lecturers a much wider range of tests and challenges. Multiple choice questions have been included for the first time and an innovative figure test has been introduced to test readers’ visual understanding.
8 . Genomic Diversity: Applications in Human Population Genetics
Author: Surinder Singh Papiha (Editor), Ranjan Deka (Editor), Ranajit Chakraborty (Editor)
One of the major themes of human population genetics is assaying genetic variation in human populations. The ultimate goal of this objective is to understand the extent of genetic diversity and the use of this knowledge to reconstruct our evolutionary history. The discipline had undergone a revolutionary transition with the advent of molecular techniques in the 1980s. With this shift, statistical methods have also been developed to perceive the biological and molecular basis of human genetic variation.
Using the new perspectives gained during the above transition, this volume describes the applications of molecular markers spanning the autosomal, Y-chromosomal and mitochondrial genome in the analysis of human diversity in contemporary populations. This is the first reference book of its kind to bring together data from these diverse sets of markers for understanding evolutionary histories and relationships of modern humans in a single volume
9. Genomics Applications for the Developing World (Advances in Microbial Ecology Book 2)
Author: Karen E. Nelson (Editor), Barbara Jones-Nelson
This book evolved from the editors strong belief that the information and new developments that were evolving from the rapidly growing field of genomics and that are happening primarily in the developed world have not happened at a parallel rate in the developing world. One would have hoped that by now the technologies and approaches would have been adapted on a far greater scale. In addition to this, the associated information is not always easily accessible, and is not disseminated in a format that can become a useful reference for scientists, students and others who reside in developing countries.
10. Genomic Applications in Pathology
Author: George Jabboure Netto (Editor), Iris Schrijve
Genomic Applications in Pathology provides a state-of-the art review of the scientific principles underlying next generation genomic technologies and the required bioinformatics approaches to analyses of the daunting amount of data generated by current and emerging genomic technologies. Implementation roadmaps for various clinical assays such as single gene, gene panels, whole exome and whole genome assays are discussed together with issues related to reporting, including the pathologist’s role in interpretation and clinical integration of genomic tests results. Genomic applications for site-specific solid tumors and hematologic neoplasms are detailed, as well as genomic applications in pharmacogenomics, inherited genetic diseases, and infectious diseases. The latest iteration of practice recommendations and guidelines in genomic testing, put forth by stakeholder professional organizations such as the Association for Molecular Pathology and the College of American Pathologists, are also discussed in the volume, as well as regulatory issues and laboratory accreditation related to genomic testing.
Written by experts in the field, Genomic Applications in Pathology provides a comprehensive resource that is of great value to practicing molecular pathologists, hematopathologists, other subspecialized pathologists, general pathologists, pathology trainees, oncologists, and geneticists.
11. Recombinant DNA Technology
Author: Keya Chaudhuri
Recombinant DNA Technology focuses on the current state of knowledge on recombinant DNA (rDNA) technology and its applications. The book provides comprehensive information on the principles and concepts of rDNA technology or genetic engineering, protein expression of cloned genes, polymerase chain reaction (PCR) amplification of DNA, restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), DNA fingerprinting, and the most recent siRNA technology. It is useful for post-graduate students and teachers in the areas of molecular biology, biotechnology, genetics, microbiology, life sciences, pharmacy, agriculture, and basic medical sciences.
12. Enzymology Primer for Recombinant DNA Technology
Author: Hyone-Myong Eun
Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning.
- Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology
- Presents information in an easily accessible format to serve as a quick reference source
- Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
13. Recombinant DNA Technology
Author: Christopher Caligiuri (Author), Kritin Verma (Author), Jasmine Werry (Author), Aakash Mishra (Author), Aaron Lustig (Author)
Abstract: Recombinant DNA (rDNA) technology is a rapidly developing and promising area of biology that has the potential to manufacture better crops, create better vaccines, and cure various genetic diseases. Throughout this article, we explore the endless possibilities created with recombinant DNA technology. In fact, we investigate and evaluate novel applications of rDNA, namely the use of rDNA for gene therapy, vaccinations, antibody production, and even clean energy production. Moreover, we outline the safety implications, ethical dilemmas, and environmental implications associated with rDNA, and how to combat them. Finally, we outline computer modeling and bioinformatics, and its implications in rDNA.
14. Recombinant DNA Methodology II (Selected Methods in Enzymology)
Author: Ray Wu
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today–truly an essential publication for researchers in all fields of life sciences.
* Methods for:
* DNA isolation and cloning
* Synthesizing complementary DNA (cDNA)
* Cleaving and manipulating DNA
* Selecting useful reporter genes
* Constructing vectors for cloning genes
* Constructing expression vectors
* Site-directed mutagenesis and gene disruption
* Identifying and mapping genes
* Transforming animal and plant cells
* Sequencing DNA
* Amplifying and manipulating DNA and PCR
* Detecting DNA – protein interaction
15. Molecular Biotechnology: Principles and Applications of Recombinant DNA (ASM Books Book 34)
Author: Bernard R. Glick (Author), Cheryl L. Patten
Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book’s organization to
- Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book.
- Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture.
- Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products.
Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose.
This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.
16. Medical Biotechnology: Principles and Applications of Recombinant DNA (ASM Books)
Author: Bernard R. Glick (Editor), Cheryl L. Patten (Editor), Terry L. Delovitch (Editor)
Biotechnology is delivering not only new products to diagnose, prevent, and treat human disease but entirely new approaches to a wide range of difficult biomedical challenges. Because of advances in biotechnology, hundreds of new therapeutic agents, diagnostic tests, and vaccines have been developed and are available in the marketplace.
In this jargon-free, easy-to-read textbook, the authors demystify the discipline of medical biotechnology and present a roadmap that provides a fundamental understanding of the wide-ranging approaches pursued by scientists to diagnose, prevent, and treat medical conditions.
Medical Biotechnology is written to educate premed and medical students, dental students, pharmacists, optometrists, nurses, nutritionists, genetic counselors, hospital administrators, and individuals who are stakeholders in the understanding and advancement of biotechnology and its impact on the practice of modern medicine.
Hardcover, 700 pages, full-color illustrations throughout, glossary, index.
17. Recombinant DNA Methodology (Selected Methods in Enzymology)
Author: Ray Wu (Editor), Lawrence Grossman (Editor), Kivie Moldave (Editor)
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry.
Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher.
- Enzymes in DNA research
- DNA isolation, hybridization, and cloning
- DNA sequence analysis
- cDNA cloning
- Gene products
- Identification of cloned genes and mapping of genes
- Monitoring cloned gene expression
- Cloning and transferring of genes into yeast cells
- Cloning and transferring of genes into plant cells
- Cloning and transferring of genes into animal cells
- Site-directed mutagenesis
- Protein engineering
- Expression vectors
18. From Genes to Genomes: Concepts and Applications of DNA Technology
Author: Jeremy W. Dale (Author), Malcolm von Schantz (Author), Nicholas Plant (Author)
The latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation.
The new edition features:
- Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics.
- Clear, two-colour diagrams throughout.
- A dedicated website including all figures.
Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.