1X Phosphate-Buffered Saline (PBS) Recipe Calculator

The 1X Phosphate-Buffered Saline (PBS) Recipe Calculator simplifies the process of preparing 1X PBS, a commonly used isotonic solution in biological research. This calculator provides accurate measurements for any desired volume, allowing researchers to prepare the appropriate amount of this buffer for various applications, including Western blotting and immunoassays.

To prepare 1 liter of 1X PBS, follow the provided instructions. Add 100 mL of a 10X PBS stock solution to 900 mL of water. This results in a final concentration of 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4 in the prepared 1X PBS solution.

By inputting the desired volume into the calculator and clicking the CALCULATE button, the table will populate with the precise amounts of each component required to make the 1X PBS solution. This ensures accuracy and allows researchers to scale up or down the preparation based on their specific needs.

The 1X PBS solution concentrations are as follows:

  • NaCl: 137 mM
  • KCl: 2.7 mM
  • Na2HPO4: 10 mM
  • KH2PO4: 1.8 mM

These concentrations are vital for maintaining the appropriate osmolarity and ionic strength required for various biological assays. PBS provides a stable and buffered environment, allowing for the proper functioning of enzymes and proteins, while the specific concentrations of NaCl, KCl, Na2HPO4, and KH2PO4 ensure optimal conditions for biological reactions.

By using the accurate 1X PBS solution prepared with the help of this calculator, researchers can ensure consistent and reliable results in their experiments, contributing to the success of their biological research endeavors.


1 L RecipeDesired Volume: mL
100 mL of 10X PBS Stock Solution mL of 10X PBS Stock Solution
900 mL of Milli-Q® Water mL of Milli-Q® Water
The resultant 1X PBS has a final concentration of 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl; pH 7.4.