1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) for Western Blotting

1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) for Western Blotting is a commonly used wash buffer in various immunoassays, particularly in Western blotting. It provides an optimal environment for antibody binding and efficient washing of membranes to remove non-specific binding.

To prepare 1 liter of TBST wash buffer, you will need to follow the specified proportions. Combine 100 mL of a 10X Tris-Buffered Saline (TBS) stock solution with 1 mL of Tween® 20 detergent and dilute the mixture with 900 mL of water. The resulting solution will be a 1X TBST wash buffer.

This calculator simplifies the process of preparing the desired volume of 1X TBST working solution, whether it is for a single experiment or for the entire laboratory. By inputting the desired volume into the calculator and clicking the CALCULATE button, the table will provide the precise amounts of each component required for the preparation.

The final concentration of the components in the 1X TBST solution is as follows:

  • Tris: 20 mM
  • NaCl: 150 mM
  • Tween® 20 detergent: 0.1% (w/v)

These concentrations are critical for maintaining the appropriate buffer conditions for Western blotting. Tris serves as a buffering agent to maintain a stable pH, while NaCl provides the necessary ionic strength for antibody-antigen interactions. The addition of 0.1% Tween® 20 detergent helps in the effective removal of non-specific binding and allows proper washing of the membrane.

By using the accurate 1X TBST working solution, researchers can ensure optimal washing conditions during Western blotting experiments, leading to reliable and reproducible results.


1 L Recipe Desired Volume: mL
100 mL of 10X TBS Stock Solution mL of 10X TBS Stock Solution
900 mL of Milli-Q® Water mL of Milli-Q® Water
1 mL of Tween® 20 detergent mL of Tween® 20 detergent