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1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

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Tris-buffered saline (TBS) is a commonly used wash buffer in various immunoassays, including ELISAs, immunohistochemistry, and Western blotting. It provides an optimal environment for antibody-antigen interactions and helps in maintaining the stability of proteins during the experimental procedures. The 1X TBS working solution is prepared by diluting a concentrated stock solution of TBS to the desired volume.

To prepare 1 liter of TBS wash buffer, you will need to follow a simple protocol. Add 100 mL of a 10X TBS stock solution to 900 mL of water. This combination results in a properly balanced 1X TBS solution with the appropriate concentrations of Tris and NaCl.

To facilitate the accurate preparation of the 1X TBS working solution, you can use a recipe calculator tool. This tool allows you to input your desired volume of the TBS solution, and with a click of the “CALCULATE” button, it calculates the amounts of each component required.

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The final concentrations of the 1X TBS working solution are as follows:

  • Tris: 20 mM
  • NaCl: 150 mM

These concentrations are critical for maintaining the desired pH and ionic strength necessary for effective washing and antibody binding during Western blotting experiments.

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By using the recipe calculator provided, you can easily prepare the appropriate amount of 1X TBS working solution for your specific needs. Whether you are performing a single experiment or preparing a larger volume for the entire laboratory, this calculator ensures accurate and consistent results.

In summary, the 1X Tris-Buffered Saline (TBS) working solution is a vital component in Western blotting and other immunoassays. Its carefully balanced concentrations of Tris and NaCl provide an optimal environment for antibody-antigen interactions. With the help of the recipe calculator, you can conveniently prepare the required volume of the 1X TBS solution, ensuring reliable and reproducible results in your experiments.

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CALCULATOR FOR TBS RECIPE

1 L Recipe Desired Volume: mL
100 mL of 10X TBS Stock Solution mL of 10X TBS Stock Solution
900 mL of Milli-Q® Water mL of Milli-Q® Water
The resultant 1X TBS working solution has a final concentration of 150 mM NaCl, 20 mM Tris; pH 7.6

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