1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting
Tris-buffered saline (TBS) is a commonly used wash buffer in various immunoassays, including ELISAs, immunohistochemistry, and Western blotting. It provides an optimal environment for antibody-antigen interactions and helps in maintaining the stability of proteins during the experimental procedures. The 1X TBS working solution is prepared by diluting a concentrated stock solution of TBS to the desired volume.
To prepare 1 liter of TBS wash buffer, you will need to follow a simple protocol. Add 100 mL of a 10X TBS stock solution to 900 mL of water. This combination results in a properly balanced 1X TBS solution with the appropriate concentrations of Tris and NaCl.
To facilitate the accurate preparation of the 1X TBS working solution, you can use a recipe calculator tool. This tool allows you to input your desired volume of the TBS solution, and with a click of the “CALCULATE” button, it calculates the amounts of each component required.
The final concentrations of the 1X TBS working solution are as follows:
- Tris: 20 mM
- NaCl: 150 mM
These concentrations are critical for maintaining the desired pH and ionic strength necessary for effective washing and antibody binding during Western blotting experiments.
By using the recipe calculator provided, you can easily prepare the appropriate amount of 1X TBS working solution for your specific needs. Whether you are performing a single experiment or preparing a larger volume for the entire laboratory, this calculator ensures accurate and consistent results.
In summary, the 1X Tris-Buffered Saline (TBS) working solution is a vital component in Western blotting and other immunoassays. Its carefully balanced concentrations of Tris and NaCl provide an optimal environment for antibody-antigen interactions. With the help of the recipe calculator, you can conveniently prepare the required volume of the 1X TBS solution, ensuring reliable and reproducible results in your experiments.