23 Difference Between Batch and Continuous Culture

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  1. 23 Difference Between Batch and Continuous Culture
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23 Difference Between Batch and Continuous Culture

CharacteristicsBatch CultureContinuous Culture
DefinitionBatch culture refers to a technique used to grow microorganisms in a limited supply of nutrients, which declines when these are used up, or some other factor becomes limiting.Continuous culture refers to a technique used for the production of microbes or microbial products in which nutrients are continuously supplied to the fermenter.
Open/Closed SystemBatch culture is a closed systemContinuous culture is an open system.
Internal EnvironmentThe internal environment of batch culture is changed with the progression of the fermentation process.The environment of the continuous culture is not changed during the fermentation process.
NutrientsNutrients are added at the beginning of the process in batch culture.Nutrients are continuously added to the continuous culture throughout the process.
limiting factorNutrients become a limiting factor at some point in a batch cultureNutrients are not a limiting factor in a continuous culture
PhasesThere are three-phase such as Lag, log, and stationary phases.lag and log phases are maintained in a continuous culture.
ContinuityThe whole process is stopped when the products are formed in a batch culture.The process continues and the products are continuously removed from the fermenter in a continuous culture.
YieldThe yield of the batch culture is low.The yield of the continuous culture is significantly high.
Turnover Ratelowhigh
Labor DemandLabor demand is less in a batch cultureLabor demand is more in continuous culture.
Chance of ContaminationThe chance of contamination is less in a batch culture.The chance of contamination is high in batch culture.
Used forBatch culture is suitable for the production of secondary metabolites such as antibiotics.Continuous culture is suitable for the production of primary metabolites such as organic acids and amino acids.
Fermenter SizeLarge fermenters are used for batch cultures.Small fermenters are used for the continuous cultures.
ControlLess control over the growth of the microbes and the production of the desired product.More control on the growth and production.
Nutrient utilization processNutrient utilization process is slow.Nutrient utilization process is fast.
WashingThe fermenter is cleaned and washed before the next step of fermentation.There are no such washing steps are required.
ComplexityBatch Culture is Easy to setupNot Easy to setup.
Termination of ProcessThe process of the batch culture is stopped after the product is formed.The process is not stopped though the product is formed. Continuous removal of the product is done without stopping the process in continuous culture.
Environmental ConditionsEnvironmental conditions are not constant.Environmental conditions inside the continuous culture are maintained at constant level.
Controlling MethodsControl methods are easy and quick.Control methods are complicated and time-consuming.
Density of bacteriaChange with timeRemain same


  • https://www.aatbio.com/resources/faq-frequently-asked-questions/What-is-the-difference-between-continuous-culture-and-batch-culture-techniques
  • https://www.differencebetween.com/difference-between-batch-and-vs-continuous-culture/
  • https://www.easybiologyclass.com/batch-fermentation-vs-continuous-fermentation-process-similarities-and-differences-a-comparison-table/
  • https://www.onlinebiologynotes.com/difference-batch-fed-batch-continuous-culture-technique/
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