Answer Key of BHU PET Applied Microbiology 2014 (Part 3)
Q101. A group of bacteria which grows in extreme environmental conditions is referred as
(1) Chemolithotrophic bacteria
(2) Archaebacteria
(3) Rickettsia
(4) Actinomycetes.
Q102. In a crowded plate technique, the criteria used for the detection of a desired colony is
(1) Zone of growth
(2) “Colour change
(3) Zone of inhibition
(4) Zone of lysis
Q103. The heat sensitive components of microbial medium are sterilized by:
(1) Moist heat
(2) Dry heat
(3) Radiation
(4) Membrane filtration
Q104. Nutrients from the environment are converted to cell components through the process of:
(1) Lyophilization
(2) Lyonophorization
(3) Catabolism
(4) Anabolism
Q105. In which of the following disease does/do the pathogen thrive in anaerobic condition?
(1) Gas gangrene
(2) Tetanus
(3) Botulism
(4) All the above
Q106. In case of humans, lysine is an essential amino acid because:
(1) It is present in all proteins
(2) It is highly nutritive
(3) It is not formed in the body and has to be supplied through the diet
(4) It is required for protein synthesis.
Q107. Which of the following is not a vitamin:
(1) Folic acid
(2) Ascorbic acid
(3) Succinic acid .
(4) Nicotinic acid.
Q108. Aflatoxin is produced by the fungus belonging to the genera
(1) Penicillium
(2) Alternaria
(3) Aspergillus
(4) Rhizopus.
Q109. During fermentation, sugar is broken down into
(1) Ethanol and carbon dioxide
(2) Ethanol and oxygen
(3) Oxygen and carbon dioxide
(4) Ethanol and water.
Q110.H.E.P.A. filter is used to filter
(1) Air
(3) Soil
(2) Water
(4) Milk
Q111. Vitamins are the chemicals which are
(1) Soluble in fat
(2) All amines
(3) Required for growth and not synthesized in the body
(4) Produced by microbes
Q112. Sweet and salty foods often do not require refrigeration to prevent spoilage, because they have
(1) Low pH
(2) High osmotic pressure
(3) Naturally occurring antibiotics
(4) Toxic alkaline chemicals
Q113. The proteases used in detergents
(1) Acid proteases
(3) Neutral proteases
(2) Alkaline proteases
(4) Serine proteases
Q114. A dead body can be preserved in ice because low temperature
(1) Kills the microbes
(2) Makes the body hard and resistant to microbial attack
(3) Arrest the activity of associated microbes
(4) Inhibits the movement of microbes through ice.
Q118. In relation to optimal growth requirements of bacterium, which group would you expect to be most likely involved in human infections?
(1) Extreme halophiles
(2) Acidophiles
(3) Mesophiles
(4) Thermophiles
Q116. Which of the following physical treatments causes damage to bacterial cells through the production of free radicals?
(1) Ultraviolet light
(2) Gamma radiations
(3) Microwave irradiation
(4) Moist heat
Q117. The largest reservoir of actinomycetes is
(1) Milk
(2) River water
(3) Air
(4) Soil
Q118. The amylase that breaks 1-6 glycosidic linkage in a starch molecule is
(1) Alpha amylase
(3) Amyloglucosidase
(2) Beta amylase
(4) Pullulanase
Q119. Tetracycline group of antibiotics act by:
(1) Inhibiting the synthesis of peptidoglycan
(2) Inhibiting 50 S ribosome function
(3) Inhibiting aminoacyl t-RNA binding to ribosome
(4) Inhibiting m-RNA translation.
120. The organisms belonging to rickettsia are
(1) Photosynthetic
(2) Nitrogen fixers
(3) Obligate intracellular parasites
(4) Bacteriophages
Q121. Enzymes not subjected to regulation by induction or repression are called:
(1) Repressor enzymes
(2) Constitutive enzymes
(3) Promotor enzymes
(4) Inducible enzymes
Q122. During esterification, OH- ions for the formation of water comes, from
(1) Acid
(2) Alcohol
(3) ketone
(4) Glucose.
Q123. What is the relationship between resolving power of a microscope and wavelength?
(1) Shorter the wavelength, higher the resolving power
(2) longer the wavelength, higher the resolving power
(3) There is no relationship
(4) It depends on the convexity of the eyepiece lens.
Q124. The relationship between organisms in which one species is benefited and other is not affected is referred as
(1) Mutualism
(2) Commensalism
(3) Antagonism
(4) Parasitism
Q125. The sequence of reactions during methane production in a biogas digester is
(1) Hydrolysis-acidolysis-methanogenesis
(2) Acidolysis-Hydrolysis methanogenesis
(3) Acidolysis- mineralization-methanogenesis
(4) None of the above
Q126. Although microbes are a very good source of proteins, Single Cell protein is not popular
(1) High nucleic acid content
(2) Slow digestibility
(3) Presence of polycyclic aromatic compounds
(4) All the above
Q127. The reaction 4H2+CO2 + CH4+2H20
(1) Liberates energy
(2) Requires energy
(3) Occurs spontaneously
(4) None of the above
Q128. In Operon theory of regulation of enzyme synthesis, to prevent transcription the repressor reacts with
(1) Operator gene
(2) Promoter gene
(3) Regulatory gene
(4) Structural gene
Q129. An in vitro technique, making large amount of any DNA sequence without the need for cloning is called the
(1) DNA sequencing
(2) Southern blotting
(3) Polymerase chain reaction
(4) DNA fingerprinting
Q130. Unique properties of each amino acid determined by its
(1) Amino group
(2) R-group
(3) Peptide bond
(4) Hydrogen bond
Q131. Which is not the intrinsic factor for food spoilage?
(1) Moisture content
(2) Nutrient availability
(3) Temperature quote
(4) Physical structure
Q132. What is the order of reagents used during Gram staining?
(1) Crystal violet, iodine, safranin, decolorizer
(2) Crystal violet, iodine, decolorizer, safranin
(3) Safranin, crystal violet, decolorizer, iodine
(4) Decolorizer, crystal violet, iodine, safranin
Q133. The field of microbiology concerning the monitoring and spread of diseases is
(1) Immunology
(2) Epidemiology
(3) Anthropology
(4) Quarentology
Q134. A common inhabitant of freshwater and marine water is
(1) Spirogyra
(2) Riccia
(3) Oedogonium
(4) Cladosphora
Q135. Citrus canker is caused by
(1) Azotobacter
(3) Erwinia
(2) Salmonella
(4) Xanthomonas
Q136. Aseptate hyphae are observed in
(1) Cladosporium
(3) Cryptococcus
(2) Rhizopus
(4) Trichophyton
Q137. Lichen are the initiators of
(1) Xerosere
(3) Halosere
(2) Hydrosere
(4) Psammosere
Q139. Microbes obtain phosphorus mainly from
(1) Inorganic phosphate ions
(2) Phospholipids
(3) Nucleic acids
(4) ATP
Q140. Which of the following is a fungal sexual spore?
(1) Chlamydospore
(2) Basidiospore
(3) Blastopore
(4) Conidiospore
Q141. The indiscriminate use of antibiotics should be avoided because
(1) They are expensive
(2) They sometimes cause allergic reactions
(3) They kill useful microflora in the human body
(4) They have been priests in the body for a long time.
Q142. Hydrolysis of gelatin is an indication that the organism secrets
(1) Pectinase
(2) Amylase
(3) Invertase
(4) Protease
Q143. Blackstrap molasses is a byproduct of
(1) Beet-sugar industry
(2) Cane sugar industry
(3) Soybean industry
(4) Starch industry
Q144. An enzyme EC I.I.I.I is a
(1) Oxidoreductase
(3) Transferase
(2) Lyase
(4) Isomerase
Q148. A substance that can evoke either a humoral or cell mediated immune response is termed as
(1) An immunogen
(2) A hapten
(3) An epitope
(4) An antigen
Q146. How many high energy bonds are present in ATP?
(1) One bond
(2) Two bond
(3) Three bond
(4) None of the above
Q147. Zygomycetes differ from other fungi in having
(1) Coenocytic hyphae
(2) Peptidoglycan in their cell wall
(3) No sexual spores
(4) No asexual spores
Q148.Penicillin is rapidly degraded in
(1) Acidic medium
(3) Neutral medium
(2) Alkaline medium
(4) Deionized water
Q149. If the mixture of enzymes amylase, cellulose, pectinase and protease is incubated at suitable conditions, the enzyme that persists at the end will be
(1) Amylase
(2) Protease
(3) Cellulase
(4) Pectinase
150. An organism that use glucose as an energy source is
(1) Organo Lithotrophic
(2) Photoorganotrophic
(3) Chemolithotrophic
(4) Chemoorganotrophic
Chemo-organotrophs: These bacteria gain energy from organic compounds such as glucose and ammino acids as a source of electrons.eg; Pseudomonas pseudoflora.
