Sourav Bio 
What is a homogenizer? Homogenizer Definition Advertisements A homogenizer is a device used to mix, emulsify, and evenly distribute particles in liquids, creating a uniform and homogeneous mixture. What does a Homogenizer do? Homogenizers are utilised to combine chemical and biological materials. This frequently involves lysing cells to create a homogeneous suspension. With the addition … Read more
What is Gel Electrophoresis System? Definition of Gel Electrophoresis System Advertisements Gel electrophoresis is a laboratory technique that uses a gel as a medium to separate and analyze biomacromolecules such as DNA, RNA, and proteins based on their size and charge. An electric field is applied to move the molecules through the gel, with smaller … Read more
What is Alcohol burner? Definition of Alcohol burner Advertisements An alcohol burner is a small, portable laboratory device that uses denatured alcohol as fuel to produce a controlled flame for heating, sterilization, and experimental purposes. Working Principle of Alcohol burner Factors that affect the working principle of an alcohol burner Several factors can influence the … Read more
What is Two pan or equal arm analytical balance? Principle of Two pan or equal arm analytical balance – How does a two-pan or equal-arm analytical balance work? Parts of a Two pan or equal arm analytical balance Advertisements Additional parts that may be found on some two-pan analytical balances include: While the core components … Read more
What is Single Pan or Unequal Arm Mechanical Balance? Parts of Single Pan/Unequal Arm Mechanical Balance Advertisements A single pan or unequal arm mechanical balance consists of several key parts that contribute to its functioning and accuracy. Here is a description of the various parts: These parts work together to create a functional and reliable … Read more
What is Reversed-Phase Chromatography? Principle of Reversed-Phase Chromatography Advertisements The principle of reversed-phase chromatography (RPC) is rooted in the interaction between molecules possessing hydrophobic groups. In RPC, the stationary phase comprises a solid support material that is modified with both hydrophobic and hydrophilic groups. When a sample is introduced into the chromatographic system, the solvent … Read more
What is Liquid Chromatography? Types of Liquid Chromatography Advertisements Liquid chromatography can be classified into four main types based on the components involved in the chromatographic process: These different types of liquid chromatography offer versatility in separating and analyzing diverse compounds, catering to various research and industrial applications. Researchers select the appropriate type of liquid … Read more
What is Hydrophobic Interaction Chromatography (HIC)? Principle of Hydrophobic Interaction Chromatography (HIC) Advertisements The principle of Hydrophobic Interaction Chromatography (HIC) is based on the interactions between the hydrophobic regions of sample protein molecules and a hydrophobic stationary phase or matrix. This principle allows for the separation and purification of proteins based on their hydrophobicity. In … Read more
What is Flash chromatography? Principle of Flash column chromatography Advertisements The principle of flash chromatography revolves around the rapid movement of the eluent, a liquid, through a short glass column under gas pressure, typically nitrogen or compressed air. The glass column is packed with a stationary phase consisting of an adsorbent material with a defined … Read more
What is Cation Exchange Chromatography? Cation Exchange Chromatography Principles Advertisements The principles of cation exchange chromatography are based on the net surface charge of proteins and the concept of isoelectric point (pI). The net surface charge of a protein can change with the pH of the surrounding environment. The pI of a protein refers to … Read more
What is Anion Exchange Chromatography? Principle of Anion Exchange Chromatography Advertisements The principle of anion exchange chromatography is based on the net surface charge of proteins, which changes with the pH and is determined by their isoelectric point (pI). The pI of a protein is the pH at which it carries no net charge. Below … Read more
What is Chromatography? Chromatography Definition Advertisements Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description … Read more
What is Two-Dimensional (Crossed) lmmunoelectrophoresis? Principle of Two-Dimensional (Crossed) lmmunoelectrophoresis Advertisements The principle of Two-Dimensional (Crossed) Immunoelectrophoresis involves the combination of two distinct electrophoretic steps to separate and identify proteins based on their charge, size, and antigen-antibody interactions. The technique consists of the following steps: The principle of Two-Dimensional Immunoelectrophoresis exploits the migration characteristics of … Read more
What is Immunoelectrophoresis? Types of Immunoelectrophoresis Advertisements There are 4 types of Immunoelectrophoresis; 1. Classical immunoelectrophoresis Advantages In summary, classical immunoelectrophoresis offers the advantages of separating complex mixtures, identifying individual components, specificity in immunoprecipitation reactions, simplicity in procedure, and providing visual and informative results. 2. Counter Current Immunoelectrophoresis Counter current immunoelectrophoresis, also known as crossover immunoelectrophoresis, … Read more
What is Polyacrylamide Gel Electrophoresis (PAGE)? Definition of Polyacrylamide Gel Electrophoresis (PAGE) Advertisements Polyacrylamide Gel Electrophoresis (PAGE) is a technique used to separate and analyze proteins or nucleic acids based on their mobility in a porous polyacrylamide gel matrix under the influence of an electric field. Types of Polyacrylamide Gel Electrophoresis (PAGE) There are several … Read more
SDS-PAGE (Sodium dodecyl Sulfate) polyacrylamide gel electrophoresis is one of the methods employed in genetics, biochemistry and molecular biology for the separation of proteins based on the molecular mass of their proteins. Proteins’ electrophoretic mobility is dependent on their size. The goal of SDS-PAGE is the separation of proteins based on their dimensions.
What is Mass Spectrometry (MS)? Definition of Mass Spectrometry (MS) Advertisements Mass Spectrometry (MS) is an analytical technique used to identify the chemical composition of a sample by measuring the mass-to-charge ratio of its ions, resulting in a mass spectrum that reveals the molecular and structural information of the sample. Mass Spectrometry Principle Mass Spectrometry … Read more
What is Centrifugal force? Definition of Centrifugal force Advertisements Centrifugal force is an outward fictitious force experienced by objects moving in a circular path, directed away from the center of rotation. Centrifugal force formula The centrifugal force can be calculated using different formulas depending on the available information. If the velocity (v) of the moving … Read more
The paper chromatography (PC) can be described as a kind of planar chromatography, where the chromatography processes are performed on a special paper. PC is thought to be the most simple and the most extensively employed of chromatographic techniques due to its ability for isolation, identification, and quantitative analysis for organic as well as inorganic substances. PC was first developed in 1865 by German scientists Christian Friedrich Schonbein (1865).
constituents of a mix for an analysis that is both qualitative as well as quantitative. For this type of physical separation, the components that are to be separated are divided into two phases. One has a stationary (stationary phase) while the second (the mobile phase) is moving in a specific direction. Based on what phase is stationary and which the mobile phase, they may be of various types.
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