Bacteria Transformation Efficiency Calculator – Microbiology Note

Bacteria Transformation Efficiency Calculator

Vector Concentration (ng/ul):

Vector Volume Used (ul):

Ligation Volume (ul):

Transformation Volume (ul):

SOC Volume (ul):

Plate Spreading Volume (ul):

Total Clone Number:

Transformation Efficiency (ug/ml):

Transformation Efficiency = Number of Clones/Amount of Vector.

Instruction

TE (ug/ml) = Transformation Efficiency (ug/ml)
TCN = Total Clone Number

PSV (ul) = Plate Spreading Volume (ul)
TV (ul) = Transformation Volume (ul)
TV (ul) = Ligation Volume (ul)

LV (ul) = Transformation Volume (ul)
VV (ul) = Vector Volume Used (ul)
VC (ng/ul) = Vector Concentration (ng/ul)