Solution preparation

Bath Solution (Ca2+/Mg2+ Free) Preparation

Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11...

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This article writter by MN Editors on June 13, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Buffer preparation
Buffer preparation

Bath Solution Preparation

To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required;

ComponentAmountConcentration
KOH (mw: 56.11 g/mol)7.854 g0.14 M
HEPES (mw: 238.3 g/mol)4.766 g0.02 M
KCl (mw: 74.55 g/mol)149 mg0.002 M
EGTA (mw: 380.35 g/mol)1.902 g0.005 M
Methanesulfonic acid (mw: 96.11 g/mol)13.314 g0.14 M

Preparation Procedure

  • Prepare 800 mL of distilled water in a suitable container.
  • Add 7.854 g of KOH to the solution.
  • Add 4.766 g of HEPES to the solution.
  • Add 149 mg of KCl to the solution.
  • Add 1.902 g of EGTA to the solution.
  • Add 13.314 g of Methanesulfonic acid to the solution.
  • Add distilled water until volume is 1 L.
  • Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C

References

AAT Bioquest, Inc. (2021, June 13). Bath Solution (Ca2+/Mg2+ Free).". Retrieved from https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free
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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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