Bath Solution (Ca2+/Mg2+ Free) Preparation

MN Editors

Bath Solution Preparation

To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required;

KOH (mw: 56.11 g/mol)7.854 g0.14 M
HEPES (mw: 238.3 g/mol)4.766 g0.02 M
KCl (mw: 74.55 g/mol)149 mg0.002 M
EGTA (mw: 380.35 g/mol)1.902 g0.005 M
Methanesulfonic acid (mw: 96.11 g/mol)13.314 g0.14 M
Word Guess Challenge
Only 1% of users are able to solve this challenge.

Guess the word hidden in this story

Preparation Procedure

  • Prepare 800 mL of distilled water in a suitable container.
  • Add 7.854 g of KOH to the solution.
  • Add 4.766 g of HEPES to the solution.
  • Add 149 mg of KCl to the solution.
  • Add 1.902 g of EGTA to the solution.
  • Add 13.314 g of Methanesulfonic acid to the solution.
  • Add distilled water until volume is 1 L.
  • Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C


AAT Bioquest, Inc. (2021, June 13). Bath Solution (Ca2+/Mg2+ Free).". Retrieved from

We hope you've enjoyed reading our latest blog article! We're thrilled to see the positive response it's been receiving so far. We understand that sometimes, after going through an interesting piece of content, you might have questions or want to delve deeper into the topic.

To facilitate meaningful discussions and encourage knowledge sharing, we've set up a dedicated QNA Forum page related to this specific article. If you have any questions, comments, or thoughts you'd like to share, we invite you to visit the QNA Forum.

QNA Forum Page

Feel free to ask your questions or participate in ongoing discussions. Our team of experts, as well as fellow readers, will be active on the forum to engage with you and provide insightful answers.Remember, sharing your thoughts not only helps you gain a deeper understanding but also contributes to the community's growth and learning. We look forward to hearing from you and fostering an enriching discussion.Thank you for being a part of our journey!

Leave a Comment