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Bath Solution (Ca2+/Mg2+ Free) Preparation

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Bath Solution Preparation

To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required;

KOH (mw: 56.11 g/mol)7.854 g0.14 M
HEPES (mw: 238.3 g/mol)4.766 g0.02 M
KCl (mw: 74.55 g/mol)149 mg0.002 M
EGTA (mw: 380.35 g/mol)1.902 g0.005 M
Methanesulfonic acid (mw: 96.11 g/mol)13.314 g0.14 M

Preparation Procedure

  • Prepare 800 mL of distilled water in a suitable container.
  • Add 7.854 g of KOH to the solution.
  • Add 4.766 g of HEPES to the solution.
  • Add 149 mg of KCl to the solution.
  • Add 1.902 g of EGTA to the solution.
  • Add 13.314 g of Methanesulfonic acid to the solution.
  • Add distilled water until volume is 1 L.
  • Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C


AAT Bioquest, Inc. (2021, June 13). Bath Solution (Ca2+/Mg2+ Free).". Retrieved from https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free


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