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Bead Homogenizer | Bead Mill Homogenizers – Principle, Uses

What Is a Bead Mill Homogenizer (Bead Homogenizer)? Bead mill homogenizers can lower the size of solid particles and disseminate them uniformly throughout a sample. ...

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Sourav Bio

What Is a Bead Mill Homogenizer (Bead Homogenizer)?

Bead mill homogenizers can lower the size of solid particles and disseminate them uniformly throughout a sample. The sample is broken up by aggressively and rapidly shaking a tube containing the beads and sample. As the beads hit with the sample (reducing particle size on a macro scale) and the inside surface of the tube, homogenization occurs (reducing particle size on a micro scale.) Shear forces induced by quickly moving beads contribute to homogeneity to a lesser level.

Homogenization using a bead mill is a highly efficient approach for simultaneously processing several samples. The use of disposable bead and tube combinations in homogenizers decreases the possibility of cross contamination. Due to the fact that the procedure takes place within a closed tube, less aerosols are produced and user exposure is restricted. This can be particularly crucial when samples contain poisonous, infectious, or otherwise dangerous chemicals.

The use of a variety of bead kinds and sizes renders this approach applicable to numerous applications. Bead mills remove tiny molecules such as nucleic acids and proteins, dissociate tissue, and recover living cells. The tubes can also be used dry, making them handy for solid materials.

Type and size of beads are essential considerations when selecting bead tubes for sample processing. Larger beads can deconstruct huge or thick formations, but smaller beads are more effective for dismantling cellular components. Bead density and form are also important. Denser beads are required for more durable and difficult materials. The edges of irregularly shaped beads can help convey the force of the moving beads to a smaller surface area, aiding in the breakdown of stiff or fibrous materials.

Principle of Bead Mill Homogenizer

Bead mill homogenizers utilise beads to homogenise, which is not surprising. The beads are aggressively agitated to disturb tissue and cells. Bead mills are excellent for tissue fragmentation, and many of them have a high throughput. In addition, because there are no probes, samples are self-contained and the risk of contamination is decreased (both cross-contamination as well as contamination of the laboratory environment). Each brand of bead mill has a unique mechanism for shaking the beads, each with its own advantages and disadvantages.

Parts of Bead Mill Homogenizer

A bead mill homogenizer, also known as a bead mill or a bead agitator, typically consists of the following parts:

  1. Mill Chamber: This is the main container where the bead mill homogenization takes place. It is typically made of stainless steel or other corrosion-resistant materials.
  2. Beads: These are the small, solid particles that are used to agitate and homogenize the sample. They can be made of different materials, such as glass, zirconium, or steel, and are chosen based on the properties of the sample and the desired outcome.
  3. Motor: This is the power source that drives the bead mill. It can be electric, pneumatic, or hydraulic.
  4. Shaft: This is the rod that connects the motor to the impeller, which provides the power to spin the beads.
  5. Impeller: This is the component that agitates the beads and the sample, creating the high-shear forces needed for homogenization.
  6. Inlet and Outlet ports: These are the ports that allow the sample to enter and exit the bead mill. Some bead mills also include a pressure gauge or thermometer to monitor the process.
  7. Lid: This is the cover that fits over the top of the mill chamber, sealing it and allowing for pressure to build up during the homogenization process.
  8. Speed Control: This controls the speed of the bead mill, which can be adjusted to optimize the homogenization process.
  9. Cooling System: Some bead mills are equipped with a cooling system that can be used to keep the sample at a specific temperature during the homogenization process.
  10. Safety guards: Some bead mills are equipped with safety guards to protect the operator from injury.

Operating Procedure of Bead Mill Homogenizer

The operating procedure of a bead mill homogenizer can vary depending on the specific model and the sample being processed, but generally follows these steps:

  1. Preparing the Sample: The sample should be prepared according to the manufacturer’s instructions, which may include filtering, centrifuging, or heating the sample to the appropriate temperature.
  2. Loading the Sample: The sample is loaded into the mill chamber through the inlet port. The beads are also added to the chamber.
  3. Closing the Lid: The lid is securely fastened to the mill chamber, sealing it and allowing for pressure to build up during the homogenization process.
  4. Starting the Bead Mill: The motor is started and the beads are agitated by the impeller. The speed of the bead mill can be adjusted to optimize the homogenization process.
  5. Running the Homogenization Process: The sample is homogenized by the mechanical energy of the beads, which agitate and break down the particles or droplets in the sample. The process is run for a specific time, or until the desired particle size is achieved.
  6. Stopping the Bead Mill: The bead mill is stopped by turning off the motor.
  7. Opening the Lid: The lid is removed, and the homogenized sample is collected from the outlet port.
  8. Cleaning the Bead Mill: The bead mill should be cleaned according to the manufacturer’s instructions, which may include disassembling the bead mill, cleaning all parts with an appropriate cleaning solution, and lubricating any moving parts.
  9. Storing the Beads: The beads should be stored properly in order to prevent contamination or damage.

It is important to always follow the manufacturer’s instructions and safety guidelines when operating a bead mill homogenizer. It’s also important to wear personal protective equipment and to avoid over-filling the chamber to prevent sample from spilling out.

Advantages of Bead Mill Homogenizer

  • Because bead mill homogenizers utilise disposable consumables, the risk of cross-contamination is quite low.
  • Some bead mill homogenizers are designed to offer a higher level of sample protection than others.
  • Since there is no motor required to power a probe, bead mill homogenizers can be conveniently constructed for high-throughput applications, and the majority of them can homogenise numerous samples at once.
  • Numerous bead mill homogenizers are designed to accommodate up to 24 samples, and high-throughput bead mills are typically much less expensive than high-throughput systems utilising other technologies.
  • In addition, the utilisation of diverse bead kinds and sizes makes bead mills applicable for a variety of applications. For example, bead mills can be used to separate tissue and recover living cells, as well as to extract intact nuclei from cells.
  • Bead mills can also be used dry, making them suitable for milling or grinding solid samples.

Disadvantages of Bead Mill Homogenizer

  • Since homogenization happens within a closed tube, there is a little risk of aerosols or other exposure to the material by the user. This is particularly crucial if the sample contains poisonous, infectious, or other dangerous chemicals.
  • The largest disadvantage of bead mill homogenizers is their maximal sample size restrictions. Due to the nature of the mechanism of action, it is impracticable to design bead mill homogenizers for huge quantities; typically, they can only accommodate samples weighing a few grammes or millilitres. (There are larger milling equipment that use beads in a rotary drum, but they are dissimilar to laboratory-scale bead mills.)
  • Since they rely on collisions between beads to homogenise the sample, bead mill homogenizers may leave minute particles in the sample. If your application could be influenced by minute quantities of the bead material, then a bead mill may not be suitable. You may be able to choose an alternative bead material that does not conflict with your downstream analytical process.

Citation

APA

Sourav Bio. (January 24, 2023).Bead Homogenizer | Bead Mill Homogenizers – Principle, Uses. Retrieved from https://microbiologynote.com/bead-homogenizer-bead-mill-homogenizers-principle-uses/

MLA

Sourav Bio. "Bead Homogenizer | Bead Mill Homogenizers – Principle, Uses." Microbiology Note, Microbiologynote.com, January 24, 2023.

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