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MCQ on Blotting Technique

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What is the purpose of blotting technique?
A. To separate molecules based on size and charge
B. To detect and analyze nucleic acids or proteins in a sample
C. To transfer molecules from a membrane to a gel
D. Both A and B
Answer: D

What is the term “blotting” referring to in blotting technique?
A. The separation of molecules based on size and charge
B. The transfer of separated molecules from a gel to a membrane
C. The detection and analysis of nucleic acids or proteins in a sample
D. None of the above
Answer: B

Which type of blotting technique is used to detect specific DNA sequences?
A. Southern blotting
B. Northern blotting
C. Western blotting
D. Eastern blotting
Answer: A

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Which type of blotting technique is used to detect specific RNA sequences?
A. Southern blotting
B. Northern blotting
C. Western blotting
D. Eastern blotting
Answer: B

Which type of blotting technique is used to detect specific proteins?
A. Southern blotting
B. Northern blotting
C. Western blotting
D. Eastern blotting
Answer: C

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Which type of blotting technique is used to detect post-translational modifications of glycoproteins?
A. Southern blotting
B. Northern blotting
C. Western blotting
D. Eastern blotting
Answer: D

What is the first step in blotting technique?
A. Detection and analysis of molecules of interest
B. Separation of molecules based on size and charge
C. Transfer of separated molecules from a gel to a membrane
D. Labeling of probes or antibodies
Answer: B

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Which molecules are separated using gel electrophoresis in blotting technique?
A. DNA or RNA
B. Proteins
C. Both A and B
D. None of the above
Answer: C

What is the purpose of labeled probes or antibodies in blotting technique?
A. To separate molecules based on size and charge
B. To transfer molecules from a membrane to a gel
C. To detect and analyze molecules of interest
D. None of the above
Answer: C

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Which type of molecule is detected and analyzed using labeled probes in blotting technique?
A. DNA
B. RNA
C. Proteins
D. All of the above
Answer: A and B

Which type of molecule is detected and analyzed using antibodies in blotting technique?
A. DNA
B. RNA
C. Proteins
D. All of the above
Answer: C

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What is the advantage of blotting technique?
A. It is a highly sensitive and specific method
B. It is a fast and inexpensive method
C. It is a simple method that does not require specialized equipment
D. None of the above
Answer: A

What is the disadvantage of blotting technique?
A. It is a time-consuming method that can take several days to complete
B. It is an expensive method that requires specialized equipment and reagents
C. It is a method that requires technical expertise and experience
D. All of the above
Answer: D

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What is the range of applications of blotting technique?
A. Basic research
B. Diagnostic testing
C. Drug development
D. All of the above
Answer: D

What is the function of the transfer buffer in blotting technique?
A. To separate molecules based on size and charge
B. To transfer separated molecules from a gel to a membrane
C. To detect and analyze molecules of interest
D. None of the above
Answer: B

Which type of gel electrophoresis is commonly used in blotting technique?
A. Agarose gel electrophoresis
B. Polyacrylamide gel electrophoresis
C. Both A and B
D. None of the above
Answer: B

What is the purpose of blocking solution in Western blotting?
A. To prevent nonspecific binding of the primary antibody
B. To enhance the sensitivity and specificity of the assay
C. To increase the signal-to-noise ratio
D. None of the above
Answer: A

What is the role of chemiluminescence in Western blotting?
A. To visualize the target protein
B. To amplify the signal of the target protein
C. To label the target protein with a fluorescent dye
D. None of the above
Answer: B

What is the function of the wash buffer in blotting technique?
A. To remove unbound probes or antibodies
B. To enhance the specificity and sensitivity of the assay
C. To increase the signal-to-noise ratio
D. None of the above
Answer: A

What is the difference between dot blotting and other types of blotting techniques?
A. Dot blotting does not require gel electrophoresis
B. Dot blotting is used to detect small molecules like hormones and drugs
C. Dot blotting is a less sensitive method compared to other blotting techniques
D. None of the above
Answer: A

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