Buffer Preparations and Recipes Physiological Buffer pH Buffering Sample Preparation BioAssays Misc Cell/Culture/Growth Media Gel Electrophoresis Name of Physiological Buffer and composiotions Extracellular Saline (1x, pH 7.3) Preparation HBSS (Hank’s Balanced Salt Solution) Preparation HEPES Buffer (1 M, 7.5 pH) Preparation HHBS (Hank’s Buffer with Hepes) Preparation PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation PBS with Ca2+and Mg2+ (D-PBS) Preparation Tyrode’s Solution, Acidic Preparation Name of pH Buffer and composiotions ACES Buffer (0.1 M, 6.7 pH)Acetate Buffer (0.1 M, pH 5.0)Acetate Buffer (pH 3.6 to 5.6)ADA Buffer (0.5 M, 6.6 pH)Ammonium AcetateAmmonium Bicarbonate (50 mM, pH 7.8)Ammonium Sulfate, SaturatedBES-Buffered Saline (2X) (0.05 M, pH 6.95)Bicine (1 M, pH 8.26)Bis-Tris Buffer (1 M, 6.5 pH)Borate BufferCAPS (0.5 M, pH 10.4)Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)CHES (0.5 M, pH 9.5)Citrate Buffer (0.1 M, pH 6.0)Citrate Buffer (pH 3.0 to 6.2)Citrate-Phosphate Buffer (0.15 M, pH 5.0)Citrate-Phosphate Buffer (20 mM, pH 5.6)Citrate-Phosphate Buffer (50 mM, pH 5.6)Diethanolamine (1 M, pH 9.8)EBSS (magnesium, calcium, phenol red) (pH 7.0)Glycine (0.1 M, pH 2.2)Glycine-HCl Buffer (0.1 M, pH 3.0)Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)HEPPSO (1 M, pH 7.85)Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)Imidazole-HCl Buffer (0.05 M, pH 7.0)Maleic Acid (1 M, pH 7.5)MES (0.5 M, pH 6)MOPS Buffer (10X) (0.2 M, pH 7)MOPSO Buffer (0.5 M, 6.9 pH)Phosphate Buffer (pH 5.8 to 7.4)PIPES Buffer (1 M, 6.8 pH)Potassium Phosphate Buffer (1 M, pH 6.5)Potassium Phosphate (pH 5.8 to 8.0)Sodium Acetate (3 M, pH 5.2)Sodium Borate Buffer (1 M, pH 8.5)Sodium Phosphate SolutionSSC buffer (20X) (3 M, pH 7)TAE (1 M, pH 8.6)TB BufferTBS (1 M, pH 7.4)TES Buffer (1 M, 7.5 pH)Tricine (1 M, pH 8.05)Tris Buffer (1 M, pH 7.2)Tris-Buffered Saline (TBS, 0.1 M)Tris-citrate Buffer (pH 8.0)Trizma® Buffer (pH 7.0 to 9.2) Name of Sample Preparation and composiotions ACK Lysis Buffer Preparation Alkaline Sodium Thiosulfate Preparation BAC DNA Microinjection Buffer Preparation DEAE-dextran Preparation EDTA Solution Preparation Elution Buffer Preparation Gey’s Balanced Salt Solution Preparation H-50 Buffer Preparation Lysis Solution Preparation Osmium Tetroxide Solution Preparation Paraformaldehyde Solution (8%) Preparation TE Buffer 10X Preparation Trichloroacetic Acid (TCA) Preparation Name of Bioassay Buffer and composiotions Anesthetic (MS222) SolutionBradford Stock Solution5-Fluorocytosine (5-FC) SolutionChloroquineCresol Red SolutionEthidium Bromide SolutionImmunofluorescence Quenching SolutionMayer’s HemalumNuclear Fast Red SolutionPyronin Y (PY) SolutionSSC (Northern Blot, 20x)SSPE (Blot, 20x, pH 7.4) Name of Misc and composiotions Dithiothreitol (DTT) (1 M)Sodium Iodide/Sodium Sulfite Solution Name of Cell/Culture/Growth Media and composiotions Bath Solution (Ca2+/Mg2+ Free)Calcium Chloride Stock SolutionCarbenicillin SolutionChelation MediumDigestion MediumEmbryonic MediumIPTG SolutionM9 Minimal SaltsModified Barth’s Saline (MBS)Penicillin/Streptomycin/Chloramphenicol Antibiotic MixYeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes Name of Gel Electrophoresis and composiotions Acrylamide Stock SolutionElectrode BufferElectrophoretic Transfer Buffer (20x)Imidazole-SDS SolutionSDS-PAGE SDS Running Buffer (10x)Sodium Carbonate Transfer Buffer (40x, pH 9.5)Tris/EACA BufferTris-Glycine Transfer Buffer (20x)
