Calcium Chloride Stock Solution Preparation

Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required.

Preparation procedure

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 58 g of CaCl2 to the solution.
3. Add distilled water until volume is 1 L.
4. Measure the osmolarity using an osmometer: if below 1000 mmol/kg, add more CaCl2; if above 1000, add H2O. (The osmolarity of 1000 mmol/kg is ~0.383 M. Preparation of this calibrated solution is necessary because calcium chloride is hygroscopic, so is not reliably measured by weight. Slight errors in calcium concentration have an outsized effect on synaptic physiology.) Store the solution at 4?C. Solution stored under these conditions appears to be stable for several months.

References

“Calcium Chloride Stock Solution Preparation.” AAT Bioquest, Inc, 13 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/calcium-chloride-stock-solution.