Solution preparation

Calcium Chloride Stock Solution Preparation

Table of Contents show 1 Calcium Chloride Stock Solution Preparation 2 Preparation procedure 3 References Calcium Chloride Stock Solution Preparation Calcium chloride...

MN Editors avatar
MN Editors
This article writter by MN Editors on June 13, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

· 32 sec read >
Buffer preparation
Buffer preparation

Calcium Chloride Stock Solution Preparation

Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required.

ComponentAmountConcentration
CaCl2 (mw: 110.98 g/mol)58 g0.5226 M

Preparation procedure

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 58 g of CaCl2 to the solution.
  3. Add distilled water until volume is 1 L.
  4. Measure the osmolarity using an osmometer: if below 1000 mmol/kg, add more CaCl2; if above 1000, add H2O. (The osmolarity of 1000 mmol/kg is ~0.383 M. Preparation of this calibrated solution is necessary because calcium chloride is hygroscopic, so is not reliably measured by weight. Slight errors in calcium concentration have an outsized effect on synaptic physiology.) Store the solution at 4?C. Solution stored under these conditions appears to be stable for several months.

References

“Calcium Chloride Stock Solution Preparation.” AAT Bioquest, Inc, 13 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/calcium-chloride-stock-solution.

microbiology note app
microbiology note app qr code Scane to download
Download Microbiology Note App Download this app for free from google play store and read ads free notes
Need a Note? Request us
Request

Leave a Reply

Your email address will not be published.

2
0

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

Join the Newsletter

Subscribe to our weekly newsletter below and never miss the latest articles.