Solution preparation

Carbenicillin Solution Preparation

Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against...

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This article writter by MN Editors on June 13, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Buffer preparation
Buffer preparation

Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall.

Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has superior stability at low pH and acts as a selection agent. Effective concentration of carbenicillin: 50 to 100 μg/ml.

Carbenicillin Solution Application

Carbenicillin is used for selection of Ampicillin-resistant transformed cells. It is used to study the role of penicillin-sensitive transpeptidases in the process cell wall biosynthesis.

Carbenicillin Solution Preparation

To prepare 1L of Carbenicillin Solution the following components are required.

ComponentAmountConcentration
disodium carbenicillin (mw: 422.36 g/mol)50 g0.1183 M

Preparation Steps

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 50 g of disodium carbenicillin to the solution.
  3. Add distilled water until volume is 1 L.
  4. Filter-sterilize using a 0.22-µm filter. Store in 1-mL aliquots at ~20°C.

References

  • “Carbenicillin Solution Preparation.” AAT Bioquest, Inc, 13 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbenicillin.
  • https://www.sigmaaldrich.com/IN/en/product/sigma/c1613
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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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