Carbenicillin Solution Preparation

By
MN Editors

Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall.

Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has superior stability at low pH and acts as a selection agent. Effective concentration of carbenicillin: 50 to 100 μg/ml.

Carbenicillin Solution Application

Carbenicillin is used for selection of Ampicillin-resistant transformed cells. It is used to study the role of penicillin-sensitive transpeptidases in the process cell wall biosynthesis.

Word Guess Challenge
Only 1% of users are able to solve this challenge.

Guess the word hidden in this story

Carbenicillin Solution Preparation

To prepare 1L of Carbenicillin Solution the following components are required.

ComponentAmountConcentration
disodium carbenicillin (mw: 422.36 g/mol)50 g0.1183 M

Preparation Steps

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 50 g of disodium carbenicillin to the solution.
  3. Add distilled water until volume is 1 L.
  4. Filter-sterilize using a 0.22-µm filter. Store in 1-mL aliquots at ~20°C.

References

  • “Carbenicillin Solution Preparation.” AAT Bioquest, Inc, 13 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbenicillin.
  • https://www.sigmaaldrich.com/IN/en/product/sigma/c1613

We hope you've enjoyed reading our latest blog article! We're thrilled to see the positive response it's been receiving so far. We understand that sometimes, after going through an interesting piece of content, you might have questions or want to delve deeper into the topic.

To facilitate meaningful discussions and encourage knowledge sharing, we've set up a dedicated QNA Forum page related to this specific article. If you have any questions, comments, or thoughts you'd like to share, we invite you to visit the QNA Forum.

QNA Forum Page

Feel free to ask your questions or participate in ongoing discussions. Our team of experts, as well as fellow readers, will be active on the forum to engage with you and provide insightful answers. Remember, sharing your thoughts not only helps you gain a deeper understanding but also contributes to the community's growth and learning. We look forward to hearing from you and fostering an enriching discussion. Thank you for being a part of our journey!

Leave a Comment