Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall.
Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has superior stability at low pH and acts as a selection agent. Effective concentration of carbenicillin: 50 to 100 μg/ml.
Carbenicillin Solution Application
Carbenicillin is used for selection of Ampicillin-resistant transformed cells. It is used to study the role of penicillin-sensitive transpeptidases in the process cell wall biosynthesis.
Carbenicillin Solution Preparation
To prepare 1L of Carbenicillin Solution the following components are required.
|disodium carbenicillin (mw: 422.36 g/mol)||50 g||0.1183 M|
- Prepare 800 mL of distilled water in a suitable container.
- Add 50 g of disodium carbenicillin to the solution.
- Add distilled water until volume is 1 L.
- Filter-sterilize using a 0.22-µm filter. Store in 1-mL aliquots at ~20°C.
- “Carbenicillin Solution Preparation.” AAT Bioquest, Inc, 13 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbenicillin.