Practical Procedure

Practical Procedure

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Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in...

Apr 9, 2021 · 3 min read >

Iron-Hematoxylin Stain Principle, Procedure, Result

Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms...

Apr 7, 2021 · 5 min read >

Acridine Orange Staining

Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular...

Apr 7, 2021 · 1 min read >

Toluidine blue stain of Mast Cell.

Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different...

Apr 6, 2021 · 1 min read >

Sudan Black B Staining

Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids...

Apr 6, 2021 · 1 min read >

Parasporal Crystal Stain Principle, Procedure, Result

Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means...

Apr 5, 2021 · 1 min read >

Bacterial Flagella Staining by Leifson Flagella Stain Method

In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from...

Apr 4, 2021 · 3 min read >

Flagella Staining Principle, Procedure, Result

Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has...

Apr 3, 2021 · 2 min read >

Capsule Staining Procedure, Principle, Result

Capsule staining is a differential staining method, where two different stains are used such as the primary stain and...

Apr 3, 2021 · 4 min read >

Endospore Staining Principle, Procedure, Result

There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.

Apr 2, 2021 · 3 min read >

Negative Staining Principle, Procedure, Result

In negative staining method, an acidic dye is used known as India Ink or Nigrosin. When the bacterial cells...

Apr 1, 2021 · 2 min read >

Simple Staining Procedure, Principle, Result

The main purpose of simple staining is to determine the cell shape, size, and arrangement of bacterial cells.

Mar 31, 2021 · 2 min read >

Kinyoun stain (Acid Fast Cold) Method: Principle, Procedure, Result

Kinyoun method also known as Kinyoun stain (cold method), was first developed by Joseph J. Kinyoun.

Mar 30, 2021 · 2 min read >

Bacterial Smear Preparation

A bacterial smear is a thin layer of bacterial cell/culture which is placed on a clean, grease-free microscopic slide...

Mar 29, 2021 · 2 min read >

Ziehl Neelsen Stain Principle, Procedure, Result

Ziehl Neelsen stinging method is used to differentiate between the acid-fast and non-acid fast bacteria.

Mar 28, 2021 · 4 min read >

Gram Staining Procedure, Principle, and Results

Gram-staining used to differentiate between Gram-Positive and Gram-Negative Bacteria.

Mar 28, 2021 · 4 min read >

Study of Chlamydomonas and Volvox by using Whole mount

Chlamydomonas are single-celled green flagellates while Volvox is a part of colonial phytoflagellate that develops a hollow spheroid with...

Jan 2, 2021 · 1 min read >

Study of Rhizopus by using Temporary Mount.

Rhizopus is a multicellular, saprophytic fungi and found on plants. They are parasitic on animals.

Jan 2, 2021 · 1 min read >

Study of Penicillium using Temporary Mount

Penicillium can be found in a freezer that has not been cleaned for a duration of 2 months or...

Jan 1, 2021 · 3 min read >

Practical Procedure

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