Practical Procedure

Aug 4 · 4 min read

Ornithine Decarboxylase Test - Principle, Procedure, Result

Decarboxylase Test Medium base is used to distinguish bacteria based on their ability to decarboxylate amino acids.

Aug 4 · 4 min read

Decarboxylase Test Principle, Procedure, Results, Uses

Aug 3 · 8 min read

Decarboxylase Test Principle, Procedure, Results, Uses

Moeller introduced the decarboxylase media to detect the ability of bacteria to decarboxylate amino acid. They were used for the first time to detect lysine, ornithine, and arginine decarboxylases among Enterobacteriaceae bacteria.

Aug 3 · 8 min read

Decarboxylase Broth Protocol

Aug 3 · 4 min read

Starch is a polysaccharide complex that is abundant in plants and typically found in the form of massive granules that are found in the cell’s cytoplasm. Starch is a carbohydrate used to store energy in plants. It’s made up of two elements Amylopectin and Amylose. composed of alpha-D-glucose that is repeated and joined to glycosidic

Aug 3 · 4 min read

Starch Hydrolysis Test – Principle, Procedure, Results, Uses

Jul 27 · 7 min read

Starch Hydrolysis Test: Principle, Procedure, Results, Uses

Soil contain different types of microorganisms which are listed below; Bacteria: Bacteria is the key soil workforce. They are the last stage in the process of breaking down nutrients before releasing them into the root zone of the plant. In fact, they are the final stage of breaking down nutrients. Food and Agriculture Organization once

Jul 27 · 7 min read

Isolation of Microorganisms from Soil

Sourav Bio

Jul 23 · 2 min read

Sourav Bio

Jul 23 · 2 min read

Ring Precipitation Test Principle, Procedure, Result

To determine the presence or absence of antigens or antibodies in a patient, many tests have been developed that use the interaction of antibodies and antigens. These tests require specificity and sensitivity. Specificity refers to the property of only binding to one antigen. Sensitivity means that the antibody can recognize and bind the antigen.

Jul 21 · 3 min read

Precipitin Ring Test Principle, Procedure, Result

The main reaction of immune system and immunology is the interaction between antibody and antigens. This is the reason to defend the body from bacterial and viral infections as well as other toxins. Its body’s defence mechanism detects foreign substances, also known as antigens, and generates specific antibodies against them. The antibodies bind with the

Jul 21 · 3 min read

Ouchterlony Double Immunodiffusion Method

Jul 9 · 8 min read

Ouchterlony Double Immunodiffusion Method

Single Radial Immunodiffusion also referred to as Mancini technique is a quantitative immunodiffusion technique that is used to measure the antigen's concentration by measuring the size of the precipitin-based ring created by the interaction between the antigen with the antibody at the optimal concentration. The antibody is embedded in the agarose gel, while the antigen is diffused into it...

Jul 9 · 8 min read

Radial Immunodiffusion Principle, Procedure, Result

Jul 8 · 6 min read

Radial Immunodiffusion Principle, Procedure, Result

Hopkin’s Cole/Adamkiewicz–Hopkins Test Definition Hopkins Cole Test also referred to as glyoxylic Acid Reaction is among the qualitative testing methods for determining the differences between different types of amino acids and proteins. The test is used to determine the presence of tryptophan, an amino acid, in proteins. It was first described in the work of

Jul 8 · 6 min read

Hopkins Cole test (Adamkiewicz–Hopkins) Principle, Procedure, Result

Jul 5 · 3 min read

Hopkins-Cole test (Adamkiewicz–Hopkins) Principle, Procedure, Result

What is the Ninhydrin Test? The ninhydrin chemical test is used for determining whether an analyte contains any amines or amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The formation of a deep blue color within the test

Jul 5 · 3 min read

Ninhydrin Test Principle, Procedure, Result, Uses

Jul 5 · 5 min read

Ninhydrin Test Principle, Procedure, Result, Uses

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10

Jul 5 · 5 min read

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Jul 2 · 11 min read

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

Haemocytometry is a quantitative method that quantifies the RBC count in blood samples. The manual method of counting RBCs is the Haemocytometer (or Neubauer’s chamber slides). Modern devices, such as photometric and electrometric counters, can be more precise or automated. They can count the cells in the blood sample. It is impossible to count the

Jul 2 · 11 min read

Total Red Blood Cell (RBC) Count – Procedure, Principle, result

Jul 1 · 10 min read

Total Red Blood Cell (RBC) Count - Procedure, Principle, Result

Despite all the technical advances in scientific laboratories, the Neubauer Chamber is still the most widely used method for cell counting worldwide. This technical data sheet was created to assist experienced and novice researchers in performing a cell count using a Neubauer or Hemocytometer. These principles can be applied to any cell-counting chamber. However, the

Jul 1 · 10 min read

Hemocytometer and Haemocytometry

Jun 30 · 8 min read

The Phenylalanine Deaminase (PDA) test can be utilized to identify Gram-negative bacilli that are urea-positive due to the capacity of microorganisms to generate phenyl-pyruvic acids through the process of oxidative deamination. The test is also referred to as the phenyl-pyruvic acids (PPA) test. Phenylalanine is an amino acid that when deaminated through oxidase enzymes, produces

Jun 30 · 8 min read

Phenylalanine Deaminase Test Principle, Procedure, Result

Jun 13 · 4 min read

Phenylalanine Deaminase Test Principle, Procedure, Result

A compound microscope is a class of optical or light microscope. I have already discussed about basics of a microscope on my previous note “Parts of Microscope with their Functions and Working Principle”, you can check them to get an idea about from which class compound microscope belongs and what is a microscope, and more.

Jun 13 · 4 min read

Compound Microscope Parts, Diagram Definition, Application, Working Principle

Jun 6 · 9 min read

A viral transport medium (VTM) is an essential nutrient that is used to transport and sustain the viability of samples (viruses) to an laboratory for analysis and identification of the specimen.

Jun 6 · 9 min read

Viral Transport Media (VTM) Definition, Principle, Preparation, Application

Jun 6 · 9 min read

Viral Transport Media (VTM) Definition, Principle, Preparation, Application

Nylander’s Test is a type of chemical test used to determine the presence of reducing sugars. Sugars like fructose and glucose can reduce bismuth oxynitrate down to bismuth when under alkaline conditions. If Nylander’s test reagent which is comprised of bismuth Nitrate along with potassium sodium tartrate as well as potassium hydroxide mixed into a

Jun 6 · 9 min read

Nylander’s Test for Carbohydrates Principle, Procedure, Result

May 23 · 1 min read

Nylander’s Test for Carbohydrates Principle, Procedure, Result

The Osazone test is a type of biochemical test which is used to detect reducing sugars. This test can distinguish between different types of reducing sugars by the appearance time of the complex. Osazone test can also be called Phenyl hydrozine test depending on the reagent used. Purpose To detect reducing sugars. To distinguish between

May 23 · 1 min read

Osazone Test for Carbohydrates Principle, Procedure, Result

May 22 · 2 min read

Osazone Test for Carbohydrates Principle, Procedure, Result

Unsaturated fish oils are especially vulnerable to oxidation, resulting in peroxides when stored in cold or freezing conditions for storage. Peroxides are precursors to breakdown products that can cause rancid taste in fat. Peroxide levels are an indicator of oxidation in the initial stages of lipid degradation. The index is less reliable in the latter

May 22 · 2 min read

Peroxide Value Test Principle, Procedure, Result

May 20 · 3 min read

May 20 · 3 min read

Get Study notes related to practicals of microbiology.

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