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Preparation of Solid Media – Agar deep tubes, Agar Slants, Plates

Preparation of Solid Media - agar deep tubes, Agar Slants, Plates

If the broth medium is supplemented with agar-agar it is referred to as agar medium like the nutrient Agar Medium (NAM) for the cultivation of bacteria potato dextrose (PDA) medium for the cultivation and storage of general fungi Czapek Dox agar (CDA) medium for fungi, starch-casein (SCA) media for the cultivation of actinomycetes, and so on.

Lysogeny broth (LB) Preparation

Lysogeny broth (LB) Preparation

LB is the most widely used bacterial culture medium but its roots lie in the area of genetics of bacteriophage. Guiseppi Bertani developed the LB recipe when he was trying to increase the amount of plaque that formed on an indicator strain of Shigella (Bertani 1952). As per Bertani, LB has been often misinterpreted to mean “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”; however the original acronym stood as “Lysogeny Broth” (Bertani, 2004). The agar version of the medium is identified as LA however, it is frequently called LB. While initially developed to study bacteriophage in addition to Shigella growth, LB subsequently became the most preferred medium to grow Escherichia bacteria and other species of the enteric.

Preparation of Liquid Medium/broth

Preparation of Liquid Medium/broth

The process of growing an organism in the surface of a medium is known as culture. The food source that supports the development of the organism is known as a culture medium. The media for culture are designed so that the organism will receive all the nutrition requirements. However, the media for culture are made in laboratories by weighing and dispensing particular ingredients or by purchasing ready-made media on the market. The majority of the media include organic as well as inorganic nutrients. However, to cultivate a variety of microorganisms, special media are created. If the media is to be solidified required, agar-agar can be mixed with other ingredients. The culture media can be classified into three categories that include semi-synthetic, natural and synthetic media each of which is employed for microbiological studies.

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.

Method for Balancing in Laboratory – Weight or Mass Measurement

Method for Balancing in Laboratory - Weight or Mass Measurement

The most frequent tasks in the microbiology area is the determination of the weight or mass of desired substances, chemicals. Another important aspect to be considered is the preservation of substances. Thus, the powders, as well as other granular or paste-like substances should Chamber not be placed directly on the platform for weighing of the balance. It is recommended that glazed paper or a small weigh boats is the best choice to weigh. It is recommended to use glazed papers if the material is 15 grams or less needs to be weighed. A the weigh boat pan or small beaker must be utilized if you are weighing a larger amount. Because of the the light weight of glazed papers its weight is subject to a minimal. If the weight is less of 1 gram an electronic balance is recommended. The larger amount (above 1 grams) is not to be considered using electronic balances.

Affinity chromatography Principle, Types, Steps, Applications

Affinity Chromatography Definition, Principle, Components, Steps, Applications

Chromatography is a crucial biophysical method that allows an identification, separation and separation of the constituents of a mixture to enable quantitative and qualitative analysis. Chromatography is an separation method that involves causing a mobile phase that is carrying a mixture is made to move into contact with a stationary phase.

Types of Spectroscopy with Definition, Principle, Steps, Uses

Types of Spectroscopy with Definition, Principle, Steps, Uses

It is the science of studying the interactions between light and matter , where the emission and absorption of the light radiations by the material are investigated and assessed. The focus of spectroscopy is the dispersion of light as well as other radiations caused by the object, which allows the examination of the various characteristics that the objects.

X-Ray Crystallography

X-Ray Crystallography

Crystallography using X-rays is a highly effective non-destructive method for determining how molecules form the crystal. The X-ray crystallography technique utilizes the principles of X-ray diffraction in order to examine the specimen, however it’s conducted in a variety of directions, so it is possible that the 3-D structures of the sample can be constructed. It is a method that has been used to determine three-dimensional crystal structures of numerous substances, particularly biological ones.

Electron Spin Resonance (ESR) – Principle, Instrumentation, Applications

Electron Spin Resonance (ESR) Principle, Instrumentation, Applications

Electron Spin Resonance (ESR) commonly referred to by the name Electron Magnetic Resonance (EMR) or Electron Paramagnetic Resonance (EPR) is an absorption spectroscopy where radiation with frequencies within the microwave range (0.04 to 25 cm) is absorption by paramagnetic materials to cause changes in the magnetic levels of electrons having unpaired spins.

Measurement Of Microorganisms – Micrometry

Measurement Of Microorganisms - Micrometry

A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.

20 Tools Used in Microbiology Laboratory

Tools Used in Microbiology Laboratory

The most commonly used equipment is inoculation needles, transfer loops, inoculation, Bunsen burner, autoclave (or pressure cooker) incubators, hot air oven centrifuge, spectrophotometer magnetic stirrer electric shaker and rotary shaker heating plate, heating mantle distillation plant, UV-lamp carbon dioxide cylinder, water-bath and a single-pan balance that has weights (for general use) chemical balance, fine analytical balance pH meters, Quebec colony counter, Laminar air flow, camera lucida electrophoresis and a high-quality microscope and many more. To perform photomicrography, a photomicrographic camera mounted in a microscope equipped with every accessory is essential in the microbiology lab.

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native Polyacrylamide Gel Electrophoresis (PAGE)

Electrophoresis with agarose or other polyacrylamide gels is an established method to separate the biopolymers, determine their identity and purify them because both of these gels have a porous the natural world. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.

Biochemical Test of Bacillus cereus

Biochemical Test of Bacillus cereus

Biochemical Test of Bacillus cereus ­Basic Characteristics Properties (Bacillus cereus) Catalase Positive (+ve) Citrate Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) Growth in KCN Positive (+ve) Hemolysis Positive (+ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Variable Oxidase Negative (-ve) Pigment Negative (-ve) Shape Rods Spore Positive … Read more

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