Chloroquine solution

• Molecular Formula of Chloroquine solution: C18H26ClN3 • 2H3PO4
• Melting point of Chloroquine solution: 193 °C
• It is a member of the quinoline family with multiple applications. 
• Solutions can be sterilized by autoclaving or by filtration through a 0.2 µm membrane. Solutions of pH 4-6 are stable when heated, but are sensitive to light.

Chloroquine solution Uses

• It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.
• It is also an antibiotic.4,5 It can be used at 200 mg/ml (PBS, pH 5.0) to dissociate antigenantibody complexes without denaturing red blood cell antigens.
• A more recent usage is for DNA transfection.7,8 When used at 100 µM, it intercalates into DNA, increasing tranfection efficiency.

Required components for Chloroquine solution

The following components are required to prepare 1L of Chloroquine solution;

Component	Amount	Concentration
chloroquine diphosphate (mw: 515.86 g/mol)	52 g	0.1008 M

Chloroquine solution Preparation

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 52 g of chloroquine diphosphate to the solution.
3. Add distilled water until volume is 1 L.
4. Sterilize the solution by passing it through a 0.22-µm filter; store the filtrate in foil-wrapped tubes at -20°C.

References

• AAT Bioquest, Inc. (2021, May 26). Chloroquine.”. Retrieved from https://www.aatbio.com/resources/buffer-preparations-and-recipes/chloroquine
• https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c6628pis.pdf