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Comparison Between Gram Stain and Acid Fast

Gram stain refers to a staining technique for the preliminary identification of bacteria, in which a violet dye is applied, followed by...

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This article writter by SouravBio on March 29, 2021

Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

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Comparison Between Gram Stain and Acid Fast
Comparison Between Gram Stain and Acid Fast

Comparison Between Gram Stain and Acid Fast

S.NoCharacteristicsGram StainAcid Fast
1DefinitionGram stain refers to a staining technique for the preliminary identification of bacteria, in which a violet dye is applied, followed by a decolorizing agent and then a red dye.The acid-fast stain refers to a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium.
2PurposeGram stain is used to distinguish between gram-positive bacteria and gram-negative bacteria.A cid-fast stain is used to distinguish between acid-fast bacteria and non-acid fast bacteria.
3Type of characterizationGram stain characterizes bacteria with different types of cell wallsAcid-fast stain characterizes bacteria with mycolic acid in the cell wall.
4Primary StainCrystal violet is used as a primary stain in Gram staining.Carbol fuchsin is used as a primary stain in Acid Fast staining.
5MordantIodine is used as mordant in Gram staining.Phenol acts as a mordant in Acid Fast staining.
6CounterStainSafranin is used as a counterstain in Gram staining.Methylene blue is used as a counterstain in Acid Fast staining.
7AppearingIn Gram stain, Gram-positive bacteria appear in blue color while Gram-negative bacteria appear in red color.In acid-fast stain, acid-fast bacteria appear in red color while non-acid-fast bacteria appear in blue color.
8MicroorganismsExample of Gram-Positive Bacteria: Lactobacillus, Nocardia, Bacillus, Clostridium, Staphylococcus, Propionibacterium, Actinomyces, Streptomyces, Enterococcus, Listria,Gardnerella, Cornyebacterium, Mycoplasma, Streptococcus.
Example of Gram-Negative Bacteria: Helicobcater, Escherichia, Shigella, Hemophilus, Neisseria, Pseudomonas, Klebsiella, Enterobacter, Vibrio, Chlamydia, Salmonella.
Nocardia spp (Partial Acid Fast), Rhodococcus spp (Partial Acid Fast), Legionella micdadei (Partial Acid Fast in tissue), Cyst of Cryptosporidium (Acid Fast), Cyst of Isospora (Acid Fast)

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Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

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