Comparison Between Kinyoun Stain vs Ziehl neelsen stain
By
MN Editors
•
Kinyoun stain vs Ziehl neelsen Stain
Characteristics
Kinyoun stain
Ziehl Neelsen Stain
Primary Stain
In Kinyoun stain method basic fuchsin is used as a primary stain.
In Ziehl Neelsen Stain method, carbol fuchsin is used as a primary stain.
Phenol concentration
A high concentration of Phenol is used.
A low concentration of phenol is used.
Heating
During the staining procedure cells are not heated.
Cells are heated for 5 minutes during the staining procedure.
Concentration of Primary stain
A high concentration of primary stain is used.
A low concentration of primary stain is used as compared to kinyoun stain.
Mordant
High concentration of Phenol and basic fuchsin act as a mordant and help in the penetration of stain into the cell.
Phenol and heat act as a mordant and help in penetration of stain into the cell.
Decolorization
The primary stain is not removed by the acid-alcohol.
The primary stain is removed by the decolorizer.
Toxic effect
Kinyoun stain method is safe as compared to the Ziehl Neelsen Stain method because in this method the phenol is not heated.
In Ziehl Neelsen Stain method phenol is heated as a result it giving rise to toxic form that can harm our eye and mucus membrane.
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