Culture media Composition

Culture Media Composition and Preparation

Cell culture is among the main methods used in the field of life sciences. It’s the term used to describe the removal of tissues, cells or organs from animals or plant, and their subsequent transfer to an artificial environment suitable for their growth and survival. The basic requirements for cells to develop optimally include controlled temperature as well as a suitable substrate for attachment, and a suitable growth medium and an incubator that has the correct pH and Osmolality. The most essential and important stage in the process of cell culture is choosing the right medium for growth In vitro cultivation. A growth medium , also known as a culture medium is a liquid or a gel created to facilitate an increase in the size of microorganisms cells, or tiny plants. The majority of cell culture meadia are the right energy source and other compounds that regulate the cycle of cells. A typical culture media includes a mixture of amino acids and vitamins, as well as inorganic salts and glucose and serum to provide a source of hormones, growth stimulants in addition to attachment factor. Along with nutrients, the medium helps to maintain the pH and the level of osmolality.

To get to know more about bacterial culture media visit this article; Bacterial Culture Media Classification, Ingredients, Functions and Preparation.

Media Name
Nutrient broth
Nutrient Agar Media Composition, Preparation, Uses
Alkaline Peptone Water Preparation, Composition, Principle, Application.
Peptone Water Composition, Preparation, Uses, Principle
Blood Agar Definition, Preparation, Composition, Application, and limitation.
Sheep blood agar Composition, Principle, Procedure, Uses.
Mueller Hinton Agar (MHA) Composition, Principle, Preparation
Salmonella Shigella Agar (SS Agar) Principle, Composition, Application
Anaerobic Culture Media Examples, Definition
Robertson’s Cooked Meat Medium (RCM Medium) Preparation, Composition.
Lowenstein Jensen (LJ) Media Composition, Preparation, Uses
Potato Dextrose Agar (PDA) Principle, Composition, Preparation, Uses
Chocolate Agar Media Principle, Composition, Preparation, Result
Xylose Lysine Deoxycholate (XLD) Agar Composition, Preparation, Principle, Uses
Mannitol Salt Agar (MSA)
Starch Casein Agar (SCA)- Composition, Principle, Preparation
New York City Agar Composition, Preparation, Result, Uses
Martin Lewis Agar Composition, Preparation, Principle, Uses
Modified Thayer Martin Agar Composition, Preparation, and Principle
Streptococcus Selective Agar Composition, Preparation, and Principle
Lactobacilli MRS Broth and Agar Composition, Preparation, Principle
Sorbitol MacConkey Agar Composition, Preparation and Uses
Peptone Yeast Extract Glucose Broth
Cystine Tryptic Agar Composition, Preparation, Principle, Uses
Cetrimide Agar Composition, Principle, Preparation, Results, Uses
Brilliant Green Agar Composition, Principle, Preparation, Results, Uses
Hektoen Enteric Agar Composition, Principle, Preparation, Results, Uses
Yersinia Selective Agar Composition, Principle, Preparation, Results, Uses
Cary Blair Transport Medium Composition, Principle, Preparation, Results, Uses
Deoxycholate Citrate Agar (DCA) Composition, Principle, Preparation, Results, Uses
Cystine Glucose Blood Agar Composition, Principle, Preparation, Results, Uses
Heart Infusion Agar Composition, Principle, Preparation, Results, Uses
Plate Count Agar (PCA) Composition, Principle, Preparation, Results, Uses
Czapek’s Agar (CZA) Composition, Principle, Preparation, Results, Uses
Anaerobic Blood Agar Composition, Principle, Preparation, Results, Uses
TCBS Agar Composition, Principle, Preparation, Results, Uses
CLED Agar Composition, Principle, Preparation, Results, Uses
CHROMagar Composition, Principle, Preparation, Results, Uses
Brain Heart Infusion (BHI) Agar- Composition, Principle, Preparation, Results, Uses
Campylobacter Blood Agar (CVA) Composition, Principle, Preparation, Results, Uses
Loeffler Medium Composition, Principle, Preparation, Results, Uses
Phenylethyl Alcohol Agar – Composition, Preparation, Uses, Limitation
Common Fungal Culture Media and Their Uses
Sabouraud Dextrose Agar (SDA)
Bird Seed Agar Composition, Principle, Preparation, Results, Uses, Limitations
Brucella Agar Composition, Principle, Preparation, Results, Uses
Ashdown’s Agar Composition, Principle, Preparation, Results, Uses
VRBA Composition, Principle, Preparation, Results, Uses
Baird Parker Agar Composition, Principle, Preparation, Results, Uses
Tryptic Soy Agar Composition, Principle, Preparation, Results, Uses
Thioglycollate Broth Composition, Principle, Preparation, Results, Uses
Amies Transport Medium Composition, Principle, Preparation, Results, Uses
Charcoal Selective Medium Composition, Principle, Preparation, Results, Uses
Selenite F Broth Composition, Principle, Preparation, Results, Uses
Selenite F Broth Composition, Principle, Preparation, Results, Uses
Lysine Iron Agar (LIA) Composition, Principle, Preparation, Results, Uses
Levinthal’s Medium Composition, Principle, Preparation, Results, Uses
EMB Agar Composition, Principle, Preparation, Results, Uses
YEPD Agar Composition, Principle, Preparation, Results, Uses
BCYE Agar Composition, Principle, Preparation, Results, Uses
Egg Yolk Agar Composition, Principle, Preparation, Results, Uses
Bismuth Sulphite Agar (BSA) Composition, Principle, Preparation, Results, Uses
Endo Agar Composition, Principle, Preparation, Results, Uses
Middlebrook Agar Composition, Principle, Preparation, Results, Uses
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