Culture Media Composition and Preparation
Cell culture is among the main methods used in the field of life sciences. It’s the term used to describe the removal of tissues, cells or organs from animals or plant, and their subsequent transfer to an artificial environment suitable for their growth and survival. The basic requirements for cells to develop optimally include controlled temperature as well as a suitable substrate for attachment, and a suitable growth medium and an incubator that has the correct pH and Osmolality. The most essential and important stage in the process of cell culture is choosing the right medium for growth In vitro cultivation. A growth medium , also known as a culture medium is a liquid or a gel created to facilitate an increase in the size of microorganisms cells, or tiny plants. The majority of cell culture meadia are the right energy source and other compounds that regulate the cycle of cells. A typical culture media includes a mixture of amino acids and vitamins, as well as inorganic salts and glucose and serum to provide a source of hormones, growth stimulants in addition to attachment factor. Along with nutrients, the medium helps to maintain the pH and the level of osmolality.
To get to know more about bacterial culture media visit this article; Bacterial Culture Media Classification, Ingredients, Functions and Preparation.