What is Euglena?

• Euglena is a type of tiny living thing found in ponds and small bodies of water. These creatures are so small that you need a microscope to see them. They are like the builders of the pond’s ecosystem, playing different roles depending on the situation.
• Sometimes, Euglena act like little plants. They have a special part in their bodies called chloroplasts that help them use sunlight to make food – just like how plants do. This makes them turn green, and they become part of the green stuff you might see on the pond’s surface.
• But at other times, Euglena act more like animals. They move around and eat the green stuff or even other tiny things around them. So, they can be both plant-like and animal-like.
• Scientists used to argue about whether Euglena should be called plants or animals. Some said they were like animals because they could move, while others said they were like plants because they could use sunlight to make food. This made Euglena a bit confusing to classify.
• Euglena are not alone. They belong to a small group of similar creatures. Some of their close relatives can even cause diseases like sleeping sickness and Chagas disease.
• These tiny organisms have a unique history. They were some of the first things people saw under a microscope. Scientists have studied a particular kind called Euglena gracilis a lot. It’s like a model that helps scientists understand how living things respond to stress.
• Euglena have a few special features that make them interesting. They can change their shape, which is pretty cool. They also have long tails called flagella that help them move around. And the most famous thing about them? They have a little red spot that looks like an eye. It’s called a stigma.
• So, the next time you see scum on a pond, you might be looking at these amazing shape-shifters, the Euglena. They might be small, but they have a big role in the world of tiny organisms.

Definition of Euglena

Euglena is a genus of single-celled microorganisms found in water, capable of photosynthesis and movement.

General Characteristics of Euglenoid

Euglenoids, a captivating group of unicellular organisms, exhibit a rich tapestry of characteristics encompassing their structure, habitat, habits, reserve food, and reproduction. This multifaceted profile sheds light on their intricate nature and underscores their significance in the realm of microorganisms. Let’s explore the defining traits of euglenoids.

Structural Versatility:

1. Blending Dualities: Euglenoids straddle the realms of both animals and plants. Their unique structure showcases a fusion of animal-like and plant-like attributes, making them a fascinating subject of study.
2. Evolutionary Advancement: Euglenoids hold a more advanced evolutionary position than blue-green algae. They feature a distinct, easily stained nucleus and localized chlorophyll in chloroplasts, akin to higher plants. The nuclear envelope remains intact during cell division, accentuating their cellular complexity.
3. Flagella: Navigational Wonders: All euglenoids boast one or two flagella, enabling graceful swimming. Positioned at the anterior end, these flagella facilitate movement and environmental interaction.

Habitat and Habits:

1. Diverse Habitats: Euglenoids flourish as free-living inhabitants of freshwater ponds, ditches, and damp soil. Their adaptability to various environments renders them a common presence in aquatic ecosystems.
2. Lab-Friendly Protists: Among protists, Euglena shines as an accessible and favored candidate for laboratory investigations, contributing to scientific advancements.

Nutritional Diversity:

1. Photosynthetic Expertise: Many euglenoids embrace a green, photosynthetic lifestyle, resembling plants. They harness sunlight for energy, utilizing chloroplasts containing pigments like chlorophyll a, chlorophyll b, and xanthophyll.
2. Varied Nutritional Modes: Euglenoids exhibit remarkable versatility in their nutritional strategies. While some adopt holophytic (photoautotrophic) nutrition, others showcase saprobic tendencies or even animal-like phagotrophic habits.
3. Paramylum Reserves: These organisms store carbohydrates in the form of paramylum, a unique compound distinct from starch and glycogen. This reserve ensures survival during times of scarcity.

Reproductive Prowess:

1. Asexual Mastery: Euglenoids primarily reproduce through asexual mechanisms, notably longitudinal binary fission. In this process, the flagellum regresses prior to division, highlighting their intricate cellular orchestration.
2. Cyst Formation: Under favorable conditions, euglenoids form cysts characterized by robust stratified membranes, often displaying a striking red hue due to the synthesis of haematochrome.

In essence, euglenoids encompass an array of captivating attributes that define their existence. From their structural amalgamation of plant and animal traits to their dynamic nutritional and reproductive strategies, these microorganisms unveil a realm of biological complexity waiting to be unraveled. Examples like Euglena, Phacus, Peranema, and others exemplify the intricate diversity within the euglenoid family.

Classification of Euglena

Domain	Eukaryota
Kingdom	Protista
Superphylum	Discoba
Phylum	Euglenozoa
Class	Euglenoidea
Order	Euglenales
Family	Euglenaceae
Genus	Euglena

Characteristics of Euglena

Euglena is a type of microorganism with distinct characteristics that make it quite intriguing to scientists. Let’s delve into its unique features in simple and formal language.

1. Wide Range of Species: The Euglena genus encompasses a large group containing 152 different species. This variety makes Euglena an important subject of study among microorganisms.
2. Flagellated Nature: Euglena is an organism equipped with tiny whip-like tails called flagella, which help it move around. Interestingly, unlike many other organisms, Euglena does not have a stiff cell wall.
3. Chloroplasts and Energy: While Euglena lacks a cell wall, it typically possesses well-defined chloroplasts. These specialized structures, akin to those found in plants, allow Euglena to perform photosynthesis. Through this process, Euglena can produce its own energy using sunlight.
4. Diverse Eating Habits: In certain conditions, Euglena’s diet can change. When sunlight is absent and organic matter is present, Euglena consumes food much like other protozoans. This adaptable behavior has sparked discussions among scientists, leading to the question of whether Euglena is more like a plant or an animal.
5. Remarkable Adaptability: Euglena has the unique ability to change its characteristics. Exposure to an antibiotic called streptomycin or heat can cause Euglena to lose its chlorophyll. In simpler terms, it can transition from a plant-like state to an animal-like one.
6. Significance in Evolutionary Study: Euglena is a significant microorganism to consider when tracing the origins of plants and animals. Its mix of traits positions it as a potential candidate for representing an early form from which both plants and animals may have evolved.

Euglena’s Dual Nature:

• Plant-like Characteristics:
  • Presence of chloroplasts containing chlorophyll.
  • Capable of holophytic (photosynthetic) nutrition, just like plants.
• Animal-like Traits:
  • Possession of a pellicle, a protective layer made of proteins (not cellulose).
  • Presence of specialized structures such as the stigma and paraflagellar body, which react to light.
  • Utilization of a contractile vacuole for managing water levels – a feature absent in plants.
  • Reproduction through a process known as longitudinal binary fission.

In essence, Euglena’s unique blend of attributes makes it a captivating microorganism to study. Its ability to adapt, transformative tendencies, and potential role in the evolutionary history of life make Euglena a subject of great scientific interest.

Euglena Habitat

The habitat of Euglena showcases an impressive adaptability that spans across diverse environments, ranging from saltwater to freshwater and even moist soils. Let’s explore the breadth of Euglena’s habitat and the strategies it employs to endure various conditions.

Versatile Environments:

1. Aquatic Abundance: Euglena species are widely dispersed across aquatic landscapes, including both saltwater and freshwater habitats. Their presence is often marked by captivating blooms that grace the surfaces of ponds and lakes. These blooms, characterized by accumulated and proliferating algae, lend vibrant colors like red or green to the water’s surface, visible even to the unaided eye.
2. Survival Strategy: Euglena’s habitat adaptability stems from its remarkable survival tactics. When faced with adverse environmental conditions, Euglena employs a strategic approach. It envelops itself in a protective wall, entering a dormant phase known as a cyst. This cyst acts as a shield, allowing the organism to endure harsh surroundings until conditions improve.

Exploring Extremes:

1. Extreme Survivors: Fascinating discoveries have illuminated Euglena’s prowess in surviving extreme conditions. Certain Euglena species exhibit remarkable thermal tolerance, enduring temperatures nearing 45°C. Additionally, others demonstrate resilience in the face of formidable challenges, such as high metal concentrations and acidic environments. For instance, Euglena mutabilis thrives at a pH as low as 1.3.
2. Nature’s Extremes: Euglena’s habitat exploration isn’t confined to the ordinary. Strains of Euglena have been uncovered in acidic hot mud pools nestled near volcanic landscapes, like those in Costa Rica. These pools, with temperatures ranging from 35 to 98°C and pH levels spanning 2 to 4, stand as testament to Euglena’s adaptability in the face of nature’s most extreme settings.

In essence, Euglena’s habitat is a realm of versatility, from serene aquatic surfaces to tumultuous volcanic landscapes. Its ability to flourish in various conditions and even withstand the harshest of environments underscores its resilience and adaptability. Euglena’s habitat, a theater of survival, speaks volumes about the intricate balance between organisms and their surroundings.

Structure and Anatomy of Euglena

Euglena, a fascinating microorganism, boasts a unique structure that distinguishes it from other living beings.

1. Elongated Shape: Euglena takes on an elongated or spindle-like form, with a size ranging from 15 to 500 micrometers. To put this into perspective, 1 centimeter is equivalent to 10000 times a micrometer. This distinctive shape is a hallmark of the Euglena genus.
2. Pellicle and Flexibility: Rather than a traditional cell wall, Euglena features a pellicle, a protein-based layer that surrounds the cell. This flexible and contractile pellicle not only helps maintain the cell’s shape but also enables it to change and contract. The stripes within the pellicle allow Euglena to flex and move, contributing to its unique appearance.
3. Photosynthetic Abilities: Euglena’s internal makeup is designed to harness the power of sunlight. It houses chloroplasts – specialized structures responsible for photosynthesis – which set it apart from other organisms. Unlike plants and green algae that have chloroplasts with two membranes, Euglena’s chloroplasts are enclosed by three membranes. These chloroplasts contain pyrenoids, unique to Euglena, which synthesize paramylon, a carbohydrate akin to starch. Paramylon serves as a food reserve for the cell, crucial for its survival in low-light conditions.
4. Eyespot and Photoreceptor: Euglena possesses a fascinating eyespot, an organelle filled with carotenoid pigments. This structure filters sunlight before it reaches the photoreceptor, known as the paraflagellar body, situated at the base of the flagellum. This interplay of the eyespot and photoreceptor plays a pivotal role in detecting light and directing the cell’s movement.
5. Flagella for Locomotion: Euglena showcases two distinct flagella at its front end. One flagellum is short and remains within the cell, while the other is long and extends outward. The longer flagellum primarily facilitates movement, propelling Euglena through its environment. In some instances, the motion generated by the flagellum aids in creating currents that bring food particles closer to the cell.

Euglena’s intricate structure combines elements of plants and animals, making it a remarkable subject of scientific study. Its ability to adapt, perform photosynthesis, and navigate its surroundings using specialized structures showcases the marvels of the natural world. This distinctive microorganism continues to capture the curiosity of researchers as they uncover its secrets.

Internal Structure and Components of Euglena

Inside a typical photosynthetic Euglena, numerous specialized structures work together to enable its functions and characteristics. Let’s delve into these internal components.

1. Pellicle: Euglena is encased in a thin and flexible membrane called the pellicle. This protective layer supports the plasma membrane and contributes to the organism’s ability to change shape.
2. Plasma Membrane: Acting as a selective barrier, the plasma membrane shields the cell’s inner content from the external environment. It controls the passage of substances in and out of the cell.
3. Cytoplasm: The jelly-like substance that fills the cell, known as cytoplasm, houses various organelles and structures. It serves as a medium for cellular processes.
4. Chloroplast: Euglena’s chloroplasts contain the green pigment chlorophyll. These vital structures enable the organism to perform photosynthesis, converting sunlight into food.
5. Nucleus: Enclosed within a membrane, the nucleus houses the cell’s genetic material, DNA. It also includes a nucleolus, which contains RNA and aids in protein synthesis.
6. Mitochondria: Responsible for producing energy in the form of ATP, mitochondria are essential for cellular activities like movement and reproduction.
7. Ribosomes: Comprising RNA and proteins, ribosomes play a pivotal role in producing cellular proteins, which are crucial for various functions.
8. Golgi Apparatus: This organelle manufactures, stores, and transports large molecules such as carbohydrates and proteins within the cell.
9. Endoplasmic Reticulum (ER): The ER is an extensive network of membranes that helps package and transport proteins to different organelles. It can be rough (RER) with attached ribosomes or smooth (SER) devoid of ribosomes.
10. Lysosomes: Small, membrane-bound organelles filled with hydrolytic enzymes, lysosomes break down large molecules like carbohydrates, fats, and proteins.
11. Contractile Vacuole: This vital structure regulates water and salt balance within the cell, ensuring proper functioning.
12. Reservoir: Found near the base of the flagella, the reservoir is an inward pocket. It assists the contractile vacuole in expelling excess water from the cell.
13. Flagellum: Euglena features a long whip-like structure called a flagellum, externally attached to the cell body. This essential appendage facilitates cell movement.
14. Eyespot: Also known as a stigma, the eyespot is a highly colored region containing red carotenoid pigments. It aids in light detection and navigation.
15. Photoreceptor (Paraflagellar Body): Located near the flagellum, the photoreceptor detects light. It influences Euglena’s movement in response to light stimuli, a process called phototaxis.
16. Paramylon: Euglena stores starch-like carbohydrate granules known as paramylon. Produced during photosynthesis, paramylon acts as a food reserve, allowing the organism to survive in low-light conditions. When encased in a polysaccharide sheath, it’s called the paramylon sheath of pyrenoid.

In conclusion, Euglena’s internal structure is a complex network of specialized components, each contributing to its unique characteristics and functions. These intricacies make Euglena a captivating subject of scientific exploration.

Nutrition of Euglena

1. Photosynthesis and Beyond: Euglena’s chloroplasts, a gift from a green algal ancestor through an endosymbiotic relationship, are its powerhouse. They perform photosynthesis, using sunlight to produce energy. However, Euglena’s dietary needs go beyond just sunlight.
2. Craving for Nutrients: While Euglena can harness sunlight, it also craves other essential nutrients. Organic substances and vital vitamins like vitamin B12 are on its menu to supplement its energy requirements.
3. Dual Lifestyles: Some photosynthetic euglenoids face an interesting twist. In the absence of light, they shed their chlorophyll and turn to a heterotrophic lifestyle. They seek nourishment from organic matter in their environment, adapting to their surroundings.
4. Heterotrophic Euglena: Certain Euglena species have adopted a fully heterotrophic lifestyle. They source their sustenance by either absorbing organic compounds from their surroundings or by engulfing bacteria and protists through phagocytosis within food vacuoles.
5. Paramylon and Energy: Euglena’s chloroplasts harbor pyrenoids, instrumental in synthesizing paramylon – a unique ꞵ-1,3 polymer of glucose. Paramylon acts as a stored food reserve, providing energy when sunlight is unavailable.
6. Toxic and Beneficial Alkaloids: In an intriguing twist, some Euglena species produce an alkaloid known as euglenophycin. While this compound holds the potential to eliminate fishes, it also highlights the complexity of Euglena’s biochemical capabilities.
7. Cultivation and Commercialization: In countries like Japan, Euglena is cultivated on a large scale for commercial paramylon production. This underscores its significance in various industries.
8. Rich in Nutrients: Remarkably, certain Euglena species are a source of vitamin E (⍺-tocopherol) and boast high astaxanthin content, contributing to their nutritional value.

Locomotion and Phototaxis movement of Euglena

1. Eyespot: A Guiding Light: Euglena is equipped with an eyespot, or stigma, a specialized region containing photoreceptors. This structure plays a pivotal role in detecting light, a process known as phototaxis, guiding the microorganism’s movement.
2. Photoreceptor Precision: When light strikes the eyespot, this visual information is channeled to a specific area known as the paraflagellar body. This integration of light signals contributes to the precise orientation of Euglena’s movement.
3. Flagella: Propellers of Motion: Euglena’s primary mode of locomotion is facilitated by one or two flagella. These whip-like appendages serve as propellers, propelling the microorganism through its aquatic habitat.
4. Origin of Flagella: Most Euglena species sport two flagella that originate from a small reservoir within the cell. This intricate arrangement contributes to the microorganism’s agility and control over its movement.
5. Dynamic Flagellar Roles: Within the flagella pair, there exists a distinction in function. A shorter flagellum remains nestled within the cell, while a longer flagellum emerges to create a dynamic and coordinated swimming motion.

In essence, Euglena’s locomotion and phototaxis movement are orchestrated by an intricate interplay between its sensory structures and specialized flagella. This dance of light detection, signal integration, and coordinated propulsion allows Euglena to gracefully navigate its watery realm, showcasing the remarkable synergy between biology and physics.

Reproduction of Euglena

Euglena’s journey of reproduction unfolds through two remarkable processes – binary and multiple fission, all while abstaining from sexual reproduction. This intricate life cycle exemplifies the microorganism’s ability to adapt and multiply.

Binary Fission:

1. Protective Encystment: Euglena demonstrates its resilience by encasing itself in a protective wall when conditions turn unfavorable. This encysted stage serves as a shelter during adverse times.
2. Daughter Generation: From this encysted state, a longitudinal split occurs, initiating the birth of two separate daughters. The process commences from the previous end and progresses backward.
3. Nucleus Division: The nucleus, a key component, elongates and undergoes division into two distinct entities.
4. Complete Separation: As the splitting continues, the individual Euglena ultimately divides into two halves. Each half receives one of the daughter nuclei, ensuring that the next generation is well-equipped.

Multiple Fission:

1. Prolific Nucleus Division: During the encysted stage, Euglena’s nucleus undergoes frequent divisions. This results in the creation of numerous tiny daughter nuclei, paving the way for a multitude of offspring.
2. Cytoplasmic Transformation: Within the encysted stage, the cytoplasm undergoes transformation. Small portions encase each daughter nucleus, giving rise to a multitude of minute creatures referred to as flagellate.
3. Emergence and Transformation: Under favorable conditions, the flagellate escape the cyst’s confines. They briefly pass through an amoeboid stage before maturing into adult Euglena.

In summary, Euglena’s reproduction is a testament to its adaptability and resilience. Through binary fission, it carefully generates two offspring with precision, while multiple fission yields a prolific array of flagellate. These distinct methods underscore Euglena’s capacity to multiply and thrive under varying environmental circumstances.

Why Euglena is known as Mixotrophs?

Euglena, a remarkable microorganism, holds the distinction of being a mixotroph, a classification reserved for organisms showcasing multiple modes of nutrition. This dual nutritional strategy further adds to the intrigue surrounding Euglena’s biology. Let’s delve into why Euglena is rightfully recognized as a mixotroph.

Understanding Mixotrophy

Mixotrophs stand out for their ability to employ more than one method of obtaining sustenance. This versatility allows them to harness nutrients from diverse sources, reflecting their adaptability and resourcefulness.

Euglena’s Nutritional Dualism

Euglena earns its status as a mixotroph due to its adeptness at both autotrophic and saprotrophic modes of nutrition.

• Autotrophic Prowess: Euglena demonstrates autotrophic nutrition akin to plants. Just as plants produce their own sustenance through photosynthesis, Euglena utilizes sunlight to synthesize its own food, harnessing energy from its chloroplasts.
• Saprophytic Strategy: On the other hand, Euglena showcases saprophytic nutrition. This mode involves feeding on other organisms to secure carbon, a fundamental element for its survival. Euglena adeptly absorbs organic nutrients and vitamins from its surroundings.

In essence, Euglena’s mixotrophic nature signifies its capability to thrive by embracing both self-sufficiency and reliance on external resources. This dynamic approach to nutrition reinforces Euglena’s position as a captivating subject of scientific inquiry, unveiling the intricacies of its biological ingenuity.

Are Euglena Harmful?

While Euglena is an intriguing genus with a multitude of fascinating features, it’s important to address a specific aspect concerning its potential harm and toxicity. Let’s delve into this important consideration.

Toxicity of Euglena sanguinea:

Within the diverse Euglena genus, there exists a species that raises concerns due to its toxic nature. Euglena sanguinea has been identified as capable of producing an alkaloid toxin known as euglenophycin, which bears a resemblance to the venom found in fire ants (Zimba et al. 2017).

Unraveling the Harm:

1. Aquatic Mortalities: Instances of concern emerged when unexplained fish mortalities occurred in an aquaculture facility in North Carolina. Over 21,000 striped bass perished, baffling experts with the absence of specific pathology. Examination of pond water samples revealed the presence of Euglena sanguinea, which was isolated and identified as the culprit behind the deaths (Zimba et al. 2004).
2. Toxic Effects: Euglena sanguinea’s toxic potency became evident when other fish species exposed to the isolated alga faced swift fatalities within a mere 7 minutes. To further establish its toxicity, juvenile catfish were subjected to the isolated alga, resulting in death after just 2 hours of exposure (Zimba et al. 2004).

Broader Implications:

1. Wider Impact: The toxic influence of Euglena sanguinea extended beyond the aquatic realm. In freshwater aquaculture systems, subsequent events involving various fish species, including catfish, tilapia, sheepshead, and striped bass, incurred significant losses totaling over $1,100,000 (Zimba et al. 2010).
2. Anticancer Properties: Intriguingly, research unveiled another dimension to Euglena sanguinea’s toxicity – its potential as an anticancer agent. The toxin, euglenophycin, exhibited anticancer activity by inhibiting phytoplankton growth and impairing the metabolic activity of mammalian cancerous cell lines (Zimba et al. 2010).
3. Leukemia Research: Further exploration into euglenophycin’s potential implications led to the discovery that the toxin could reduce the viability of human leukemia cells, with promising results suggesting its role in future studies involving animal models (Zimba et al. 2016).

Extending the Scope:

1. Varied Concentrations: A study conducted in 2017 expanded the investigation into euglenophycin, evaluating its presence across 12 Euglena species. Concentrations exceeding 5 femtograms per cell were found in specific species, notably Euglena clavata, Euglena anabaena, Euglena stellata, Euglena socialis, and the aforementioned Euglena sanguinea. In fact, Euglena socialis and Euglena sanguinea exhibited concentrations of euglenophycin that were significantly higher than other Euglenaceae species (Zimba et al. 2017).

In conclusion, while Euglena encompasses a wealth of intriguing attributes and potential applications, it’s important to acknowledge that certain species, such as Euglena sanguinea, possess toxic properties. The presence of euglenophycin and its implications for aquatic environments, as well as its potential as an anticancer agent, underscore the complexity and multifaceted nature of these microorganisms. Vigilance and further research remain crucial to understanding and mitigating the potential harm associated with certain members of the Euglena genus.

Importance of Euglena

Euglena holds significant importance in various aspects of biology, ecology, and potential applications. Here are some of the key reasons why Euglena is considered important:

• Ecological Role: Euglena is an essential component of aquatic ecosystems, where it plays a role in nutrient cycling. As a primary producer, Euglena contributes to the production of organic matter through photosynthesis, serving as a food source for other organisms in the ecosystem.
• Mixotrophic Nature: Euglena’s ability to switch between autotrophic (photosynthetic) and heterotrophic (absorbing nutrients) modes of nutrition makes it versatile in adapting to different environmental conditions. This flexibility is important for its survival and impacts nutrient dynamics in ecosystems.
• Research Model: Euglena gracilis, in particular, has been extensively studied as a model organism in scientific research. Its single-celled nature, rapid reproduction, and responsiveness to environmental changes make it valuable for studying cellular processes, stress responses, and evolutionary biology.
• Biodiversity and Taxonomy: Euglena contributes to the overall biodiversity of microorganisms, and its study aids in refining taxonomic classifications and understanding the diversity within the Euglenozoa phylum.
• Biotechnology and Biofuel: Certain species of Euglena, especially Euglena gracilis, are being explored for their biotechnological potential. They can accumulate valuable compounds such as lipids, proteins, and paramylon, which have applications in food, feed, and biofuel production.
• Environmental Indicators: The presence or absence of specific Euglena species in water bodies can serve as indicators of water quality and ecosystem health. Changes in Euglena populations can reflect shifts in nutrient levels, pollution, or other environmental factors.
• Nutritional Value: Euglena’s nutritional composition, including proteins, lipids, and paramylon, makes it a potential source of sustainable and nutritious food for human consumption and animal feed.
• Carbon Fixation and Climate Change: Euglena’s role in photosynthesis contributes to carbon dioxide fixation, helping to mitigate the effects of greenhouse gas emissions and climate change.
• Medicinal and Biomedical Research: Euglenophycin, a toxin produced by certain Euglena species, has shown potential anticancer properties in laboratory studies. Research into its effects could contribute to biomedical advancements.
• Education and Outreach: Euglena’s unique characteristics and behaviors make it an engaging subject for educational purposes. Studying Euglena can help students learn about cellular biology, ecology, and the interconnectedness of organisms in ecosystems.

Overall, Euglena’s ecological, physiological, and biotechnological significance underscores its importance in advancing scientific understanding, supporting ecosystem functions, and contributing to potential solutions for various societal challenges.

Euglena Under Microscope Video

Red Euglena sp.

Euglena mutabilis, showing metaboly, paramylon bodies and chloroplasts

Euglena sanguinea

Euglena, moving by metaboly and swimming

FAQ

References

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4. https://www.geeksforgeeks.org/euglenoid/
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