Solution preparation

Immunofluorescence Quenching Solution Preparation and Application

Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.

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This article writter by MN Editors on June 02, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Buffer preparation
Buffer preparation

Immunofluorescence Quenching Solution Application

Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.

Components required

The following components are required to prepare 1L of Immunofluorescence Quenching Solution.

ComponentAmountConcentration
Glycine (mw: 75.07 g/mol)3.75 g0.05 M
NH4Cl (mw: 53.49 g/mol)2.67 g0.05 M

Immunofluorescence Quenching Solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 3.75 g of Glycine to the solution.
  3. Add 2.67 g of NH4Cl to the solution.
  4. Add distilled water until volume is 1 L.
  5. Quenching solution can be stored at ~20°C for several weeks.

References

“Immunofluorescence Quenching Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.

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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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