Advertisements
Table of Contents
Immunofluorescence Quenching Solution Application
Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.
Components required
The following components are required to prepare 1L of Immunofluorescence Quenching Solution.
Advertisements
| Component | Amount | Concentration |
| Glycine (mw: 75.07 g/mol) | 3.75 g | 0.05 M |
| NH4Cl (mw: 53.49 g/mol) | 2.67 g | 0.05 M |
Immunofluorescence Quenching Solution Preparation
- Prepare 800 mL of distilled water in a suitable container.
- Add 3.75 g of Glycine to the solution.
- Add 2.67 g of NH4Cl to the solution.
- Add distilled water until volume is 1 L.
- Quenching solution can be stored at ~20°C for several weeks.
References
“Immunofluorescence Quenching Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.
