Laboratory Refrigerators and Freezers – Principle, Parts, Applications


Table of Contents

What is Laboratory Refrigerators and Freezers?

  • Laboratory refrigerators and freezers are essential equipment used in scientific and medical settings to store and preserve various samples, specimens, vaccines, pharmaceuticals, and biological materials. They are designed to meet the specific requirements of laboratory environments, ensuring the safety, reliability, and integrity of the stored items.
  • Unlike regular refrigerators found in homes or restaurants, laboratory refrigerators must adhere to higher standards of hygiene and reliability. They are required to maintain a constant temperature to minimize the risk of bacterial contamination and prevent volatile material explosions. These refrigerators incorporate features such as air circulation systems and fans to ensure consistent temperature distribution and accuracy. The fan is designed to shut off when the door is open to prevent cold air from escaping and compromising the internal environment. Moreover, laboratory refrigerators often have distinct sections to prevent cross-contamination between different samples or materials.
  • Laboratory freezers, on the other hand, are designed to provide even lower temperatures than refrigerators. They are capable of reaching temperatures as low as -80ºC and are used to store highly sensitive biological samples, chemicals, and other temperature-sensitive materials. Laboratories, hospitals, blood banks, production facilities, material testing labs, and diagnostic centers rely on laboratory freezers to maintain the integrity and viability of their stored samples.
  • The temperature ranges for laboratory refrigerators and freezers differ based on their intended purpose. Typically, laboratory refrigerators store samples within the range of -5ºC to -15ºC, while laboratory freezers maintain temperatures between -25ºC and -15ºC. The differentiation between refrigerators and freezers is primarily based on the temperature range they can achieve.
  • In addition to storage, some laboratory refrigerators and freezers can also function as incubators, capable of alternating between heating and cooling cycles. These specialized refrigeration units are utilized for culturing and monitoring the growth of microorganisms under controlled conditions.
  • Overall, laboratory refrigerators and freezers play a critical role in preserving and protecting valuable scientific and medical samples. Their hygienic design, precise temperature control, and reliable performance ensure the integrity of these samples, enabling researchers, healthcare professionals, and scientists to carry out their work effectively and safely.

Definition of Laboratory Refrigerators and Freezers

Laboratory refrigerators and freezers are specialized equipment used in scientific and medical settings to store and preserve samples, specimens, vaccines, pharmaceuticals, and biological materials at specific temperatures. They ensure hygienic conditions, precise temperature control, and reliable performance to maintain the integrity and viability of the stored items.

Working Principle of Laboratory Refrigerators and Freezers

Parts/Components of Laboratory Refrigerators and Freezers
Parts/Components of Laboratory Refrigerators and Freezers | Image Source: scienceabc.

The principle behind laboratory refrigerators and freezers is based on the refrigeration cycle, also known as the vapor-compression refrigeration cycle. This cycle involves the transfer of heat from an area with a lower temperature to an area with a higher temperature using mechanical work.


The refrigeration cycle begins by compressing the refrigerant gas, which raises its temperature and pressure. The heat produced during compression is dissipated through heat exchange coils located on the exterior of the refrigerator.

As the refrigerant cools, it liquefies and passes through a safety valve. This liquid refrigerant then moves from the high-pressure region to the low-pressure region through the safety valve, where it undergoes expansion and evaporation. During this process, the refrigerant absorbs heat from its surroundings, resulting in a cooling effect.


Within the refrigerator, coils assist the refrigerant in absorbing heat, maintaining a cold interior. This process is repeated as the refrigerant continues to circulate through the system, ensuring a consistent cooling effect.

In the case of freezing, when a substance transitions from a liquid to a solid state, heat is transferred from the substance to the surrounding environment. As heat is released, the kinetic energy of the liquid particles decreases, causing them to slow down. Eventually, the particles reach a point where their mobility decreases significantly, and they are drawn together by attractive forces, leading to the formation of a solid during the freezing process.


By utilizing the principles of the refrigeration cycle and the heat transfer during freezing, laboratory refrigerators and freezers can maintain specific temperature ranges to store and preserve samples, specimens, and other sensitive materials in a controlled and reliable manner.

A Laboratory Refrigerators and Freezers Must-have features to consider

When considering laboratory refrigerators and freezers, there are several must-have features that should be taken into account. These features ensure optimal performance, energy efficiency, temperature stability, and ease of use. Here are some key features to consider:

  1. Maximum Cooling Efficiency: Look for a refrigeration system that is designed to provide maximum cooling efficiency. This ensures that the unit can reach and maintain the desired temperature quickly and effectively.
  2. Energy Efficiency: Opt for a refrigerator or freezer that consumes low power, as this can lead to substantial energy savings over time. Energy-efficient models not only reduce operating costs but also contribute to environmental sustainability.
  3. Forced Air Circulation: A laboratory refrigerator or freezer should have a system of forced air circulation. This feature promotes improved temperature homogeneity throughout the unit, ensuring that all stored items are kept at a consistent temperature. Additionally, it aids in rapid temperature recovery following door openings, minimizing temperature fluctuations.
  4. Intelligent Defrost Function: An intelligent automated defrost function is essential. This feature ensures that the unit only defrosts when necessary, based on specific temperature and time criteria. By defrosting only when needed, it helps maintain the integrity of stored samples while minimizing energy consumption.
  5. User-Friendly Controls: Look for a refrigerator or freezer with user-friendly and intuitive control methods. This may include a digital control panel with clear temperature displays and easy-to-use settings. Intuitive controls simplify operation and allow for precise temperature adjustments.
  6. Insulated Door with Magnetic Gasket Seal: A laboratory refrigerator or freezer should have a well-insulated door equipped with a magnetic gasket seal. The insulation helps prevent temperature fluctuations caused by external factors, while the magnetic gasket ensures a tight seal, minimizing the ingress of warm air and maintaining temperature stability.
  7. LED Lighting: Opt for a unit with LED lighting. LED lights not only conserve energy but also have a longer lifespan compared to traditional fluorescent lighting. This feature provides clear visibility of the stored contents while minimizing heat generation inside the unit.

Parts/Components of Laboratory Refrigerators and Freezers

Parts/Components of Laboratory Refrigerators and Freezers
Parts/Components of Laboratory Refrigerators and Freezers | Image Source: scienceabc.

1. Refrigerant

  • Refrigerant is a crucial component of refrigeration systems, including refrigerators and freezers. It plays a vital role in the cooling process by undergoing phase changes from gas to liquid and back to gas as it circulates through the various components of the system. The refrigerant is responsible for absorbing heat from the interior of the refrigerator and releasing it to the external environment, thus facilitating the cooling effect.
  • Historically, different types of refrigerants have been used in refrigeration systems. Examples of refrigerants include freon (chlorofluorocarbons or CFCs) and ammonia. However, due to environmental concerns and their potential to deplete the ozone layer, the use of CFCs and certain other refrigerants has been phased out or heavily regulated.
  • In modern refrigerators, the most commonly used refrigerant is HFC 134a. HFC stands for hydrofluorocarbon, which is a class of refrigerants that do not contain chlorine and have a lower ozone depletion potential compared to CFCs. HFC 134a is a non-toxic, non-flammable gas that has been widely adopted in refrigeration systems due to its favorable properties, such as high thermodynamic efficiency.
  • It’s important to note that there is an ongoing focus on developing and using refrigerants with even lower environmental impact, such as hydrofluoroolefins (HFOs) and natural refrigerants like carbon dioxide (CO2) and hydrocarbons. These alternatives aim to reduce greenhouse gas emissions and minimize the environmental footprint of refrigeration systems.
  • Refrigerants are carefully selected and regulated to ensure they are safe, efficient, and environmentally friendly. Proper handling, storage, and disposal of refrigerants are essential to prevent harm to human health and the environment. Compliance with regulations and guidelines regarding refrigerant use is crucial to promote sustainability and minimize the impact of refrigeration systems on the planet
Parts/Components of Laboratory Refrigerators and Freezers
Parts/Components of Laboratory Refrigerators and Freezers

2. Compressor

  • The compressor is a vital component of a refrigerator or freezer, often referred to as the “heart” of the cooling system. Its primary function is to initiate and drive the cooling process. Powered by a motor, the compressor plays a key role in maintaining the proper temperature inside the appliance.
  • When the refrigeration cycle begins, the compressor receives the low-pressure refrigerant gas from the evaporator. It then applies mechanical work, typically through a piston or scroll mechanism, to compress the gas. As a result, both the temperature and pressure of the refrigerant increase significantly.
  • By compressing the gas, the compressor creates high pressure on the warm portion of the refrigeration circuit, enabling the transfer of heat from the interior of the refrigerator to the external environment. This process is crucial for extracting heat from the refrigerated space and facilitating the cooling effect.
  • After compression, the refrigerant leaves the compressor in a high-pressure, high-temperature state. It then enters the condenser, where it undergoes further cooling and condensation, releasing heat to the surrounding environment. The refrigerant transitions from a gas to a liquid form during this phase.
  • The compressor’s role is not limited to the initial cooling process. It continues to circulate the refrigerant throughout the refrigeration system, maintaining the necessary pressure levels for efficient heat transfer. The compressed refrigerant flows through the condenser, expansion valve, evaporator, and back to the compressor, completing the refrigeration cycle.
  • Efficient compressor operation is crucial for maintaining the desired temperature inside the refrigerator or freezer. It ensures that the refrigerant can effectively absorb heat from the interior, facilitating proper cooling and preservation of stored items.
  • Overall, the compressor is a critical component that drives the refrigeration process by compressing the refrigerant, increasing its temperature and pressure, and initiating the heat transfer necessary for cooling. Its proper functioning is essential for the overall performance and efficiency of refrigeration systems.

3. Condenser

  • The condenser is an important component of a refrigerator or freezer that is typically located at the back of the appliance. Its primary function is to facilitate the release of heat from the refrigerant, aiding in the conversion of the refrigerant from a high-pressure gas to a liquid state.
  • When the high-pressure, high-temperature refrigerant gas leaves the compressor, it enters the condenser. The condenser consists of a network of coils, often made of copper, which provides a large surface area for heat exchange. As the refrigerant flows through the condenser coils, it comes into contact with the cooler ambient air surrounding the refrigerator.
  • During this process, the refrigerant undergoes a phase change, transitioning from a gas to a liquid state. This change occurs as the refrigerant releases heat to the surrounding environment through the condenser coils. As the heat is dissipated, the refrigerant cools down and condenses into a liquid form.
  • The condenser’s design and the placement of the coils contribute to its effectiveness in transferring heat. The large surface area of the coils, along with the airflow generated by the refrigerator’s cooling fan, enhances heat exchange and facilitates efficient cooling of the refrigerant.
  • Over time, dust and debris can accumulate on the condenser coils, reducing their heat exchange efficiency. This can lead to decreased cooling performance and increased energy consumption. Regular cleaning and maintenance of the condenser are important to ensure optimal functioning of the refrigerator.
  • The condenser plays a crucial role in the overall refrigeration cycle, as it enables the release of heat absorbed from the interior of the refrigerator. By condensing the refrigerant from a gas to a liquid, the condenser helps maintain the desired temperature inside the appliance and promotes efficient cooling.
  • In summary, the condenser in a refrigerator or freezer serves to cool and condense the refrigerant, allowing for the release of heat. Its copper coils and location at the back of the appliance make it identifiable, while its efficient operation is essential for proper cooling and optimal performance.
Parts/Components of Laboratory Refrigerators and Freezers
Parts/Components of Laboratory Refrigerators and Freezers

4. Expansion Valve (Or Capillary Tube)

  • The capillary tube, also known as the expansion valve, is a crucial component in refrigeration systems, including refrigerators and freezers. It is a small, thin copper tube designed to regulate the flow of refrigerant and control the temperature and pressure within the system.
  • As the refrigerant leaves the condenser in a high-pressure liquid state, it enters the capillary tube. The capillary tube is carefully sized and designed to create a significant drop in both temperature and pressure of the refrigerant. This drop in pressure and temperature is essential for the cooling process to occur effectively.
  • The capillary tube acts as a restriction in the refrigeration system, causing the high-pressure liquid refrigerant to rapidly expand and convert into a low-pressure mixture of liquid and vapor. This process is known as throttling or expansion.
  • By expanding the refrigerant, the capillary tube allows for the evaporation of a significant portion of the liquid refrigerant. This evaporation absorbs heat from the surrounding environment, resulting in a cooling effect. The cooled refrigerant then enters the evaporator, where it further absorbs heat from the refrigerator’s interior, maintaining the desired low temperature.
  • The size and length of the capillary tube are carefully chosen to match the specific requirements of the refrigeration system. Factors such as the refrigerant type, desired cooling capacity, and system design influence the selection of an appropriate capillary tube.
  • The capillary tube serves as an important control device, regulating the flow and pressure of the refrigerant as it transitions from the high-pressure liquid state to the low-pressure vapor state. Its function is to maintain the proper balance between cooling capacity and temperature control within the refrigerator or freezer.
  • While the capillary tube is a simple and relatively inexpensive component, its proper sizing and functioning are crucial for the overall performance and efficiency of the refrigeration system. It plays a vital role in creating the cool conditions required for effective cooling and preservation of the stored items.
  • In summary, the capillary tube, also known as the expansion valve, is a small copper tube that reduces the temperature and pressure of the liquid refrigerant, leading to its evaporation. This facilitates the cooling process and helps create the desired cool conditions inside refrigerators and freezers.

5. Evaporator

  • The evaporator is a vital component of a refrigerator or freezer responsible for maintaining the desired low temperature inside the appliance. It plays a crucial role in the refrigeration cycle, facilitating the cooling process and ensuring the preservation of food and other items.
  • Located inside the refrigerator, the evaporator consists of a series of coils or fins typically made of copper or aluminum. These coils are in direct contact with the air inside the refrigerator, allowing for efficient heat transfer.
  • After the refrigerant passes through the capillary tube and undergoes expansion, it enters the evaporator as a low-pressure mixture of liquid and vapor. As the refrigerant flows through the evaporator coils, it absorbs heat from the surrounding air. This heat absorption causes the refrigerant to evaporate, converting from a liquid to a gas.
  • The evaporation process is crucial for cooling the interior of the refrigerator. As the refrigerant evaporates, it draws heat from the surrounding environment, effectively lowering the temperature inside the appliance. This cools the air circulating within the refrigerator and maintains the desired cold conditions necessary for preserving food and other perishable items.
  • The evaporator acts as a heat exchanger, allowing the refrigerant to extract heat from the interior and transfer it to the refrigeration system. The absorbed heat is carried away by the refrigerant as it continues its journey through the refrigeration cycle.
  • Once the refrigerant has evaporated and absorbed heat from the refrigerator’s contents, it returns to the compressor to begin the cycle anew. The compressor then compresses the low-pressure gas, initiating the next round of heat transfer and cooling.
  • Efficient functioning of the evaporator is crucial for maintaining proper cooling inside the refrigerator or freezer. The design of the evaporator coils, their surface area, and the airflow around them contribute to efficient heat exchange and cooling performance.
  • In summary, the evaporator is the component within a refrigerator or freezer that facilitates the cooling process. It evaporates the refrigerant, allowing it to absorb heat from the surrounding air and maintain the desired low temperature. By drawing heat away from the refrigerator’s contents, the evaporator creates an optimal environment for preserving food and other perishable items.

6. Other Parts

Accessories are additional components or features that enhance the functionality and convenience of a refrigerator. They play a crucial role in monitoring and maintaining optimal conditions within the appliance. Here are some common accessories found in refrigerators:

  1. Thermistor: A thermistor is a temperature-sensitive resistor that monitors the internal temperature of the refrigerator. It sends signals to the control system to regulate cooling and ensure the desired temperature is maintained. The thermistor helps prevent fluctuations in temperature and ensures food stays fresh.
  2. Evaporator Fan Motor: The evaporator fan motor is responsible for circulating cold air throughout the freezer and refrigerator compartments. It helps maintain consistent temperatures, prevents the formation of hot spots, and ensures proper cooling of stored items. The fan motor enhances air circulation, allowing for efficient cooling and preservation of food.
  3. Condenser Fan Motor: In refrigerators with compressor compartment mountings, the condenser fan motor drives air through the condenser coils. This assists in cooling the refrigerator and dissipating heat generated during the refrigeration process. The condenser fan motor plays a crucial role in maintaining proper cooling performance and preventing overheating.
  4. Water Inlet Valve: The water inlet valve controls the flow of water into the refrigerator’s ice maker or water dispenser. It is responsible for generating sufficient water pressure to open the valve and dispense water into the ice tray, allowing the production of ice. The water inlet valve ensures a steady supply of water for ice making and dispensing.
  5. Water Filter: Many modern refrigerators feature built-in water filtration systems. The water filter helps remove impurities and contaminants from the water supply, ensuring clean and fresh-tasting water for drinking or ice making. Regularly replacing the water filter is important to maintain water quality and prevent clogging that can affect ice production.
  6. Thermometers and Alarms: Some refrigerators are equipped with built-in thermometers and alarms. These accessories provide temperature monitoring and alert systems to ensure the refrigerator is operating within the desired temperature range. The thermometers help users keep track of the internal temperature, while alarms notify them of any temperature fluctuations or malfunctions that may impact food safety.

These accessories contribute to the overall performance, convenience, and safety of the refrigerator. They help maintain optimal temperatures, enhance air circulation, ensure clean water supply, and provide monitoring and alert systems for efficient operation.


Types of Laboratory Refrigerators

Types of Laboratory Refrigerators
Types of Laboratory Refrigerators

Laboratory refrigerators come in various types, each designed to meet specific storage requirements and ensure the safe preservation of samples, chemicals, or specialized materials. Here are some common types of laboratory refrigerators:

  1. Explosion-Proof Refrigerators: These refrigerators are specifically designed for storing flammable liquids and dangerous chemicals. They feature an explosion-proof construction, ensuring that there are no electrical appliances or potential sources of sparks inside the storage space. This eliminates the risk of fire or explosion in environments where a combustible atmosphere may occur, such as solvent dispensing rooms.
  2. Lab Fridges: Lab fridges are general-purpose refrigerators used for storing a wide range of laboratory samples and materials. They are designed to maintain stable temperatures and often feature digital temperature displays for accurate monitoring. Lab fridges typically have lockable parts and easy-to-clean interiors to ensure sample security and maintain cleanliness. They are commonly used for the preservation and cooling of various laboratory samples.
  3. Blood Bank Refrigerators: Blood bank refrigerators are specialized units used for the storage of blood and blood products. These refrigerators are equipped with precise temperature controls to maintain a consistent and optimal temperature for the preservation of chilled whole blood, blood components, and blood products. They are designed to meet the stringent requirements of blood banks, hospitals, and research facilities that handle blood-related materials.
  4. Chromatography Refrigerators: Chromatography refrigerators are specifically designed for laboratory applications that require precise temperature control and stability. They are commonly used in scientific experiments and procedures where maintaining specific temperature conditions is critical for the accuracy and reliability of results. Chromatography setups, which involve separation and analysis of components, can be housed within the controlled temperature chamber of these refrigerators.

These are just a few examples of the types of laboratory refrigerators available. Other specialized refrigerators include pharmacy refrigerators for storing medications, vaccine refrigerators for maintaining the integrity of vaccines, and ultra-low temperature freezers for long-term storage of sensitive biological samples at ultra-low temperatures. The choice of a laboratory refrigerator depends on the specific storage requirements, temperature control needs, and safety considerations of the laboratory or facility.


Types of Lab Freezers

Lab freezers are essential for preserving and storing a variety of samples, reagents, and other temperature-sensitive materials in laboratory and healthcare settings. Here are some common types of lab freezers:

  1. Ultra-Low Temperature Freezers: Ultra-low temperature freezers are designed to reach extremely low temperatures, making them ideal for storing items that require ultra-low temperature conditions. These freezers can maintain temperatures below -40°C and some models can even reach temperatures as low as -86°C. They are equipped with advanced insulation and two independent evaporator circuits to provide exceptional temperature stability and uniformity throughout the storage chamber.
  2. Chest Freezers: Chest freezers are a popular choice for labs that require storage of large items or bulk quantities of samples. They feature a horizontal, chest-like design with a top-opening lid. Chest freezers offer efficient storage space and are often equipped with alarm systems to alert users of any temperature changes or other abnormalities. They are well-suited for long-term storage and can maintain stable temperatures even during power outages.
  3. Under-Counter Freezers: Under-counter freezers are designed to fit conveniently underneath laboratory counters or benches. They come in various configurations, including all-purpose models and explosion-proof options for storing potentially hazardous materials. Under-counter freezers often feature forced air ventilation, eliminating the need for extra clearance space around the unit. They may also offer settings to control defrost frequency, preventing unexpected thaws and sample dehydration.
  4. Upright Freezers: Upright freezers have a vertical design and are equipped with shelving units inside the storage chamber. This allows for efficient organization of samples and maximizes storage capacity while taking up minimal floor space. Upright freezers provide good temperature homogeneity, ensuring that all lab samples are exposed to consistent conditions. They often feature adjustable shelves, temperature alarms, and digital temperature displays for ease of use.

Each type of lab freezer has its own advantages and is suitable for specific storage requirements. Factors such as the size of the lab, storage capacity needs, temperature range, and sample organization preferences should be considered when choosing the appropriate type of lab freezer for a particular laboratory or facility.

Laboratory Refrigerators and Freezers Operating procedures

Operating procedures for laboratory refrigerators and freezers are crucial to ensure the safety of stored materials and maintain the proper functioning of the equipment. Here are some important guidelines to follow:

  1. Avoid storing flammables: Never store flammable substances with a flash point lower than 37.8°C (100°F) in a household refrigerator. These materials should be kept in explosion-proof refrigerators specifically designed for storing flammable liquids and chemicals.
  2. Separate food and lab materials: It is essential to maintain a clear distinction between refrigerators or freezers used for laboratory purposes and those used for storing food or beverages intended for human consumption. Do not store food items in lab refrigerators or freezers to prevent cross-contamination.
  3. Verify compatibility: Before storing substances in a laboratory refrigerator or freezer, ensure that they are compatible with the materials and components of the equipment. Some chemicals or samples may react with the refrigerator materials, leading to contamination or equipment damage.
  4. Securely seal containers: All containers stored in the refrigerator or freezer should be securely positioned and tightly sealed. Avoid using aluminum foil, corks, or uncoated glass stoppers as they may not provide adequate sealing. Properly sealed containers help prevent leakage, maintain sample integrity, and minimize the risk of contamination.
  5. Use plastic trays for containment: Place plastic trays suitable for secondary containment on all shelves of the refrigerator or freezer. These trays serve as an additional layer of protection in case of spills or leaks, ensuring easy cleanup and preventing cross-contamination between samples.
  6. Proper labeling: All materials stored in the refrigerator or freezer should be appropriately labeled. Clear and legible labels help identify the contents, date of storage, and any necessary precautions or handling instructions.
  7. Limited storage duration: Store only the chemicals and samples that are needed for a reasonable period of time. Extended storage without proper maintenance and sealing may result in degradation of substances and compromise their integrity.
  8. Regular cleaning and defrosting: It is important to clean and defrost refrigerators and freezers at least once a year, or more frequently as needed. Regular maintenance helps prevent the buildup of ice, frost, or contaminants, ensuring optimal performance and extending the lifespan of the equipment.

Adhering to these operating procedures promotes safety, preserves sample quality, and enhances the overall efficiency of laboratory refrigerators and freezers. It is essential to follow any additional guidelines provided by the manufacturer and comply with relevant safety regulations and protocols specific to the laboratory or facility.

Guidelines on use of laboratory refrigerator

Guidelines on the use of laboratory refrigerators are important to ensure the proper storage and organization of samples, maintain their integrity, and promote overall laboratory efficiency. Here are some key guidelines to follow:

  1. Sample storage: Avoid exposing samples or reference materials directly in the refrigerator. Instead, store them in plastic pouches or standard vials to prevent cross-contamination that may occur due to close proximity to other samples.
  2. Light-sensitive samples: If you have light-sensitive samples, store them in amber or dark-colored vials to protect them from light exposure, which can degrade their quality.
  3. Labeling: Ensure that labels are securely fastened to the sample containers and covered with a transparent film. This helps prevent moisture condensation from rendering the labels illegible, ensuring clear identification of samples.
  4. Storage zones: Different types of materials, such as chemicals, standards, foods, and pharmaceuticals, may have specific temperature requirements. Store these items in temperature zones within the refrigerator that correspond to their specific storage needs. Avoid placing samples randomly, as this may compromise their stability and intended purposes.
  5. Naming and record-keeping: It is recommended to label the cabinets or sections within the refrigerator and maintain an updated list of sample locations. This can be done using user records or a digital inventory system. Having a current record of sample locations saves time and ensures easy retrieval when needed.
  6. Regular cleaning: Perform regular cleaning of the cabinets and shelves at least weekly. Ensure that the electricity to the refrigerator is turned off before cleaning to prevent accidents. Cleaning helps maintain a hygienic environment, prevents cross-contamination, and removes any spills or debris that may affect sample integrity.

By following these guidelines, laboratories can ensure the proper organization, protection, and maintenance of samples within the laboratory refrigerator. It is also important to adhere to any additional instructions provided by the manufacturer and comply with relevant safety protocols and regulations specific to the laboratory or facility.

Calibration of Laboratory Refrigerators and Freezers

The calibration of laboratory refrigerators and freezers is crucial to ensure accurate temperature control and reliable preservation of samples and standards. Unlike domestic refrigerators, laboratory refrigerators require regular calibration and the maintenance of calibration records. Here are some key points to consider regarding the calibration process:

  1. Calibration procedure: To calibrate a laboratory refrigerator or freezer, a calibrated thermometer is placed in a beaker filled with silicone oil. This beaker is then placed in several drawers and the freezer compartment of the refrigerator. The refrigerator door is closed, and the temperature is allowed to stabilize for approximately 15 minutes.
  2. Temperature readings: After the stabilization period, temperature readings are taken from the calibrated thermometer. For refrigerator cabinets, the temperature range should typically be maintained between 2 and 8 degrees Celsius. Freezers, on the other hand, should ideally operate within a temperature range of -10 to -20 degrees Celsius.
  3. Maintenance of calibration records: It is essential to keep detailed records of the calibration process, including the date of calibration, temperature readings, and any adjustments made. These records serve as documentation for quality control purposes, ensuring traceability and providing evidence of compliance with regulatory requirements.
  4. Importance of calibration: A well-calibrated laboratory refrigerator is essential for maintaining the integrity and stability of stored samples and standards. Accurate temperature control helps prevent temperature fluctuations that could negatively impact the quality and viability of the stored materials. Regular calibration ensures that the refrigerator operates within the desired temperature range and provides reliable storage conditions.
  5. Separation of lab and food storage: Laboratory refrigerators and freezers should never be used to store food and beverages. Unintentional contamination from chemicals or other substances stored in the refrigerator can have severe health consequences if consumed. It is crucial to maintain clear separation and label the refrigerator accordingly to prevent any mix-up or cross-contamination risks.

By following proper calibration procedures and maintaining accurate calibration records, laboratory personnel can ensure the reliable performance of refrigerators and freezers. Regular calibration helps maintain optimal storage conditions, prolongs the lifespan of samples and standards, and contributes to the overall integrity of laboratory research and analysis.

Applications of Laboratory Refrigerators and Freezers

Laboratory refrigerators and freezers find wide-ranging applications across various scientific and medical fields. Here are some key applications of laboratory refrigerators and freezers:

  1. Storage of samples and specimens: Laboratory refrigerators are crucial for preserving biological samples, such as blood samples, tissue cultures, and DNA samples, at specific temperatures. By maintaining a controlled environment, these refrigerators ensure the integrity and stability of the stored samples for future analysis and experimentation.
  2. Vaccine and medication storage: Laboratory refrigerators play a vital role in the storage of vaccines, medicines, and pharmaceutical products. These refrigerators are designed to maintain precise temperature control, ensuring the stability and efficacy of vaccines and medications, particularly those that are temperature-sensitive.
  3. Reagent storage: Laboratory refrigerators provide a controlled environment for storing reagents used in chemical analysis. They help maintain the stability and integrity of the reagents by ensuring consistent temperature control. This is essential for accurate and reliable chemical testing and experimentation.
  4. Moisture control: Some laboratory refrigerators are equipped with anti-frost fans or dehumidification systems. These features help reduce moisture levels within the refrigerator, creating a drier climate. This is particularly beneficial for storing dried reagents, moisture-sensitive materials, and samples prone to contamination from excess moisture.
  5. Microbiological media quality control: Laboratory refrigerators and freezers offer climate control capabilities to maintain the quality of microbiological media. These specialized units ensure that the temperature, humidity, and other environmental conditions are conducive to the growth and preservation of microorganisms used in research, diagnostic testing, and other microbiological applications.
  6. Long-term storage: Laboratory freezers, including ultra-low temperature freezers, are utilized for long-term storage of samples, specimens, and valuable biological materials. These freezers can maintain extremely low temperatures, allowing for the preservation of materials at temperatures as low as -86°C. This is particularly useful for storing valuable samples that require prolonged preservation without degradation.

Advantages of Laboratory Refrigerators and Freezers

Laboratory refrigerators and freezers offer several advantages that are crucial for the storage and preservation of biological materials, vaccines, and other laboratory samples. Here are some key advantages:

  1. Reliable cold chain: Laboratory refrigerators and freezers ensure a reliable cold chain, which is essential for maintaining the integrity and efficacy of biological materials, such as blood transfusions, vaccines, and other treatments. By providing consistent and controlled temperature environments, these units prevent spoilage and ensure the effectiveness of stored materials.
  2. Temperature monitoring and alarm systems: Laboratory refrigerators and freezers are equipped with accurate temperature monitoring systems and alarms. These features help prevent temperature fluctuations and provide timely alerts in case of any temperature deviations. By maintaining a stable temperature, these units ensure the safety and integrity of the stored materials.
  3. Improved airflow and ventilation: Refrigerators and freezers designed for laboratory use are equipped with powerful air circulation systems and air cooling vents. These features promote better airflow and ventilation within the storage compartments, ensuring uniform temperature distribution and minimizing temperature variations. This helps maintain optimal storage conditions for the stored materials.
  4. Access control with digital locks: Some laboratory refrigerators and freezers are designed with digital locks. These locks restrict access to authorized personnel only, ensuring the security and integrity of the stored materials. By preventing unauthorized access, the risk of tampering or contamination is minimized.
  5. Backup power sources: Laboratory refrigerators and freezers often incorporate backup battery systems. These backup power sources provide additional benefits during power outages or temporary disruptions in the electrical supply. They help maintain the required temperature conditions and prevent any temperature excursions that could compromise the stored materials.
  6. Prevention of biological contamination: By providing a controlled and sterile environment, laboratory refrigerators and freezers play a crucial role in preventing or stopping biological contamination in laboratories. They help create a barrier that protects the stored materials from external contaminants, ensuring the purity and integrity of the samples.

In summary, laboratory refrigerators and freezers offer advantages such as ensuring a reliable cold chain, temperature monitoring and alarms, improved airflow and ventilation, access control with digital locks, backup power sources, and prevention of biological contamination. These features contribute to the safe storage, preservation, and integrity of biological materials, vaccines, and other laboratory samples.

Limitations of Laboratory Refrigerators and Freezers

While laboratory refrigerators and freezers offer numerous benefits, they also have some limitations that need to be considered. Here are a few limitations associated with these devices:

  1. Risk of spillage and chemical compatibility: Laboratory refrigerators and freezers may pose risks of spillage and the emission of gases from the stored contents. Incompatible chemicals or samples placed in close proximity can interact with each other, potentially leading to reactions or contamination. It is essential to ensure proper labeling, containment, and segregation of materials to mitigate these risks.
  2. Higher operating and maintenance costs: Compared to household refrigerators, laboratory refrigerators and freezers tend to have higher operating and maintenance costs. These units often require more energy to maintain precise temperature control, resulting in increased energy consumption. Additionally, specialized maintenance and repair services may be required, which can be more costly.
  3. Environmental impact: Improper disposal of laboratory refrigerators and freezers can have adverse effects on the environment. The refrigerants used in these units, such as chlorofluorocarbons (CFCs) or hydrofluorocarbons (HFCs), can contribute to ozone layer depletion if released into the atmosphere. It is crucial to follow proper disposal procedures and adhere to environmental regulations to minimize these environmental risks.
  4. Limited storage capacity: Laboratory refrigerators and freezers often have limited storage capacity compared to larger industrial-scale units. Depending on the size and specific requirements of the laboratory, the available storage space may not be sufficient to accommodate all samples and materials. This limitation may necessitate careful organization and periodic removal of expired or unnecessary items.
  5. Temperature fluctuations during door openings: When the doors of laboratory refrigerators and freezers are opened frequently or for extended periods, temperature fluctuations can occur. This can affect the stability of stored samples and materials, especially those sensitive to temperature changes. It is important to minimize unnecessary door openings and maintain proper storage practices to mitigate this limitation.


When working with laboratory refrigerators and freezers, it is important to take certain precautions to ensure the safety and integrity of the stored samples and materials. Here are some precautions to consider:

  1. Proper containment of samples: It is crucial to avoid leaving samples or reference materials exposed in the refrigerator or freezer. To prevent cross-contamination, samples should be securely contained in plastic bags or standard vials. This practice minimizes the risk of interactions between different samples and maintains their individual integrity.
  2. Appropriate storage zones: Different materials have specific temperature requirements for storage. It is important to store chemicals, standards, foods, and medications in temperature zones that align with their specific storage needs. This ensures that the samples or substances are stored under optimal conditions and helps maintain their stability and effectiveness.
  3. Protection of light-sensitive samples: Light-sensitive samples should be given special attention. They should be stored in opaque bags or vials that are amber or dark-colored. This protects them from exposure to light, which can degrade or alter their properties. Properly storing light-sensitive samples helps maintain their quality and reliability.
  4. Secure attachment and protection of labels: Labels on stored items play a vital role in ensuring accurate identification and traceability. Labels should be properly attached to the containers and covered with a transparent film to prevent moisture condensation. This ensures that the labels remain readable and legible, allowing for easy identification of the stored samples or materials.
  5. Safe cleaning procedures: Regular cleaning of the cabinets and shelves is necessary to maintain cleanliness and prevent the buildup of contaminants. However, it is important to exercise caution while cleaning. Before cleaning, always turn off the electricity to avoid any potential hazards. Follow proper cleaning protocols and use appropriate cleaning agents to ensure the safety of both the operator and the stored samples.

Laboratory Refrigerators and Freezers Examples and Buy

Thermo Scientific MR45SS-GARE-TS Series GP Laboratory Upright Refrigerator

  • Exceptional security features
  • Outstanding temperature stability
  • Intuitive digital controls
  • Quality, robust construction

NOR-LAKE LR161WWW/0 General Purpose Laboratory Refrigerator, Upright Auto Defrost

  • Dimensions are 67-1/2″ H x 34 W x 30-1/4 D
  • Package Quantity: 1
  • Product Type: Lab Supply
  • Country Of Origin: United States

American BioTech Supply ABT-HC-UCFS-0104G Premier Undercounter Refrigerator

  • Exterior color: White
  • Refrigerant: R600a
  • Defrost: cycle
  • UL/c-ul Listed
  • Digital temperature display

HNZXIB 80L (2.7Cu Ft) Vertical Ultra-Low Temperature Freezer

  • SUS304 interior, healthy and good looking, easy cleaning; Double door gaskets provide good seal
  • Unique patented technology for auto cascade single-stage compression refrigeration system and a recipe for mixed CFC-free refrigerants
  • Superior performance: strong and quick cooling, the lower final temperature
  • World-famous brand thermostat: Italian Dixell temperature controller, high control accuracy, digital temperature display, Hi/Low temperature & sensor fault alarms, easy operation
  • EBM condenser fan motor: strong and reliable, low failure rate, long working life.Low noise

HNZXIB -86° C Horizontal Ultra-low Temperature Laboratory Freezer

  • Intelligent computer configuration makes temperature control more accurate. Microcomputer digital control, real-time temperature display, adjustment unit is 0.1℃.
  • Humanized design, easy to use. The internal controls and temperature indicating devices of the refrigerator are installed on the front of the refrigerator, which is convenient and quick to use, and has a beautiful appearance.
  • Equipped with an intelligent alarm system. Complete alarm system (over-temperature alarm system failure alarm) and temperature recording system (optional) to ensure the safe storage of items in the refrigerator.
  • The ultra-thick environmentally-friendly insulation layer effectively reduces heat loss, saves energy and prolongs service life,The inner cavity of the box is made of stainless steel, and the tubular copper tube evaporator is easy to fetch, the heat dissipation effect is good, and the inner tank is easy to clean.
  • We use express delivery. Wooden packing. 7-10 days to arrived


What is the purpose of a laboratory refrigerator or freezer?

Laboratory refrigerators and freezers are designed to store and preserve various types of samples, specimens, reagents, vaccines, and other temperature-sensitive materials in a controlled environment.

How do laboratory refrigerators and freezers differ from domestic ones?

Laboratory refrigerators and freezers are specifically designed for the unique requirements of laboratory settings. They often provide precise temperature control, better temperature uniformity, and additional safety features compared to domestic units.

Can I store food or beverages in a laboratory refrigerator or freezer?

No, laboratory refrigerators and freezers should be strictly used for laboratory purposes only. Storing food or beverages in these units can lead to contamination and compromise the integrity of both the stored items and the laboratory environment.

What temperature range is typically maintained in laboratory refrigerators?

Laboratory refrigerators typically maintain a temperature range between 2°C and 8°C. This temperature range is suitable for storing samples, reagents, and medications that require refrigeration but not freezing.

What temperature range is typically maintained in laboratory freezers?

Laboratory freezers can maintain temperatures below 0°C, typically ranging from -20°C to as low as -86°C in ultra-low temperature freezers. The specific temperature range depends on the type and purpose of the freezer.

How often should laboratory refrigerators and freezers be cleaned and defrosted?

It is recommended to clean and defrost laboratory refrigerators and freezers at least once a year or as frequently as necessary. Regular cleaning helps maintain cleanliness, prevent contamination, and ensure optimal performance.

Can laboratory refrigerators and freezers store flammable substances?

Certain laboratory refrigerators are designed to be explosion-proof and can safely store flammable substances. However, it is important to use the appropriate type of refrigerator specifically designed for this purpose to ensure safety.

How can I monitor the temperature inside a laboratory refrigerator or freezer?

Laboratory refrigerators and freezers often come with built-in temperature monitoring systems, such as digital displays and alarms. These systems allow users to monitor and maintain the desired temperature range and receive alerts if there are any temperature deviations.

Are laboratory refrigerators and freezers energy-efficient?

Efficiency levels can vary among different models and types of laboratory refrigerators and freezers. However, modern units often come with energy-saving features, such as LED lighting and efficient insulation, to minimize power consumption and reduce energy costs.

What precautions should I take when handling samples in laboratory refrigerators and freezers?

It is important to follow proper handling procedures and precautions when working with samples in laboratory refrigerators and freezers. This includes using proper containment methods, labeling samples accurately, and ensuring secure sealing to prevent cross-contamination and maintain sample integrity.



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