Mendel’s Law of Segregation – Definition, Principle, Examples, Limitations

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What is Mendel’s Law of Segregation?

Mendel’s Law of Segregation, a foundational principle in genetics, elucidates the behavior of alleles, the different forms of a gene, during the process of inheritance. Put simply, this law states that in the hybrids or heterozygotes of the first generation (F1 generation), when there are two contrasting characters – one dominant and one recessive – the alleles for these characters, though initially paired together, remain distinct and separate during the formation of gametes. This separation ensures that each gamete carries only one allele for a given trait, either dominant or recessive.

To break it down, the law emphasizes that only a single copy of a gene, inherited from one parent, is transmitted to a gamete. The allocation of these gene copies into gametes is a random process, ensuring genetic diversity and variability in subsequent generations.

Mendel’s law of segregation is built upon several crucial concepts:

  1. Allelic Forms: A gene exists in multiple variations, known as alleles. These alleles can have distinct traits or characteristics.
  2. Separation During Gametogenesis: When gametes are formed, the allelic pair of a gene segregates, or separates, so that each resulting gamete contains only one allele.
  3. Inheritance of Two Alleles: Organisms inherit two alleles for each genetic trait, one from each parent.
  4. Dominance and Recessiveness: The two alleles inherited for a trait are different in nature, where one is dominant and masks the expression of the other recessive allele.

Mendel’s law of segregation is instrumental in using Punnett squares for predicting the genotypes resulting from genetic crosses. This tool is based on the assumption of equal segregation of alleles during gametogenesis, allowing scientists to anticipate the potential outcomes of mating between organisms with known genotypes.

This law holds significant importance as it introduced the concept that hereditary factors, or alleles, remain distinct entities even when present alongside other similar entities. This concept debunked the idea of blending inheritance, a prevalent theory at the time, by demonstrating that traits encoded by recessive alleles could reappear in the F1 generation. In essence, Mendel’s experiments and his law of segregation laid the groundwork for our modern understanding of genetics and inheritance patterns, forming the cornerstone of genetic research and advancements.

Mendel’s Law of Segregation Definition

Mendel’s Law of Segregation states that alleles for a trait separate during gamete formation, ensuring each gamete carries only one allele, randomly inherited from the parent.

What is segregation?

  • Segregation refers to the fundamental genetic process in which pairs of alleles, representing different traits of the same gene, are separated and distributed into separate gametes during meiosis. This process ensures that each gamete carries only one allele for a particular trait.
  • Meiosis is a specialized cell division that occurs in sexually reproducing organisms. During meiosis, the homologous chromosomes, which carry genes that determine various traits, align and then segregate into distinct daughter cells. This segregation of alleles guarantees genetic diversity among offspring.
  • The significance of segregation lies in its contribution to genetic variation and inheritance patterns. By ensuring that each gamete receives only one allele from each gene pair, segregation allows for different combinations of alleles to be passed down to the next generation. This variation is essential for the adaptability and survival of species over time.
  • In summary, segregation is a fundamental genetic principle that ensures the separation of allele pairs during meiosis, leading to the inheritance of diverse genetic traits among offspring.

Principle of Segregation and its Importance

  • The principle of segregation is a fundamental concept in genetics that plays a pivotal role in explaining the inheritance of genetic traits. This principle, proposed by Gregor Mendel, states that individuals possess two alleles for a specific characteristic, and during the formation of gametes, these alleles segregate or separate from each other. As a result, only one allele is present in each gamete.
  • In simpler terms, if an individual carries two different alleles for a particular trait (heterozygous), such as one allele for brown eyes and another for blue eyes, these alleles separate during the formation of gametes. As a consequence, each gamete carries only one allele for that trait, either the allele for brown eyes or the allele for blue eyes.
  • The importance of the principle of segregation lies in its role in shaping the genetic diversity within populations and the inheritance patterns observed in offspring. By ensuring that each gamete contains only one allele from each gene pair, segregation contributes to the random assortment of alleles during fertilization. This random assortment generates a wide array of genetic combinations, leading to the diversity of traits in subsequent generations.
  • Furthermore, the principle of segregation is the foundation for understanding genotypic ratios and predicting the outcomes of genetic crosses. It enables scientists to use tools like Punnett squares to estimate the potential genotypes of offspring resulting from various mating scenarios.
  • In summary, the principle of segregation elucidates how alleles separate during gamete formation, leading to the presence of only one allele in each gamete. This process contributes to genetic diversity and is essential for understanding inheritance patterns, genotypic ratios, and the overall mechanism of genetic variation within populations.
Principle of Segregation and its Importance
Principle of Segregation and its Importance

Characteristics of Mendel’s Law of Segregation

Mendel’s Law of Segregation is characterized by its association with the initial stage of meiotic cell division, wherein homologous chromosomes carrying two copies of the same gene are separated into distinct daughter nuclei. This separation of homologous chromosomes during meiosis is responsible for the segregation of alleles at gene loci, leading to the formation of diverse gametes.

This mechanism becomes clearer through the example of a monohybrid cross involving tall and dwarf pea plants. For instance, if we consider a homozygous tall pea plant with the alleles RR and a short pea plant with the alleles rr:

  1. The parent with RR alleles produces gametes containing a single R allele.
  2. The parent with rr alleles produces gametes containing a single r allele.
  3. Each gamete carries only one chromosome of the homologous pair, thereby carrying a single allele.

Upon fertilization, the fusion of gametes results in a heterozygous or hybrid plant with Rr alleles, incorporating both dominant and recessive alleles. Due to incomplete dominance, the dominant R allele partially manifests itself in the first-generation hybrid, while the recessive allele remains unexpressed.

Importantly, in heterozygous individuals, both alleles persist together without influencing each other. In the context of the monohybrid cross, the combination of gametes yields three potential diploid genotypes: RR, Rr, and rr. This showcases how Mendel’s Law of Segregation operates to distribute alleles independently during gamete formation, resulting in the inheritance patterns observed in subsequent generations.

Characteristics of Mendel’s Law of Segregation
Characteristics of Mendel’s Law of Segregation

Examples of Mendel’s Law of Segregation

1. Albinism in humans

  • Albinism in humans is another illustrative example of Mendel’s Law of Segregation in action. Albinism is a genetic condition characterized by the absence of pigment production, resulting from the presence of an abnormal recessive trait.
  • In the context of albinism, the dominant allele responsible for the absence of the condition is represented as ‘A,’ while the recessive allele leading to albinism is represented as ‘a.’
  • When a cross takes place between a homozygous individual with alleles AA (non-albino) and aa (albino), the process of genetic segregation becomes evident. The resulting gametes are of two types: A and a.
  • Upon the fusion of these gametes, hybrid offspring with Aa alleles are produced. In this situation, the dominant allele ‘A’ is expressed, leading to the absence of albinism in the phenotype.
  • However, if subsequent crosses occur, resulting in hybrids with genotypes AA and aa, the ‘aa’ alleles become particularly significant. Individuals with ‘aa’ alleles exhibit albinism, as these alleles lead to the lack of production of the enzyme tyrosinase, which is essential for the synthesis of melanin, the pigment responsible for skin, hair, and eye color.
  • The example of albinism serves as a compelling demonstration of the workings of Mendel’s Law of Segregation, where alleles segregate during gamete formation and unite in various combinations during fertilization, ultimately determining the observable traits in offspring.
Albinism in humans
Albinism in humans

2. Morgan’s work on Drosophila

  • T.H. Morgan’s groundbreaking work with Drosophila, a type of fruit fly, proved instrumental in advancing our understanding of genetics. He conducted experiments involving the crossbreeding of homozygous long-winged Drosophila with homozygous vestigial-winged Drosophila, leading to significant insights into the process of genetic segregation.
  • In this experiment, let’s assume that the long-winged Drosophila carries a pair of v+v+ alleles responsible for long wings, while the vestigial-winged Drosophila possesses vv alleles linked to vestigial wings.
  • As the crossbreeding takes place, the principle of Mendel’s Law of Segregation becomes evident. The long-winged Drosophila generates gametes with a single v+ allele, and the vestigial-winged Drosophila produces gametes carrying a single v allele.
  • Upon fusion of these gametes, hybrid offspring with v+v alleles emerge. Despite carrying both alleles, phenotypically they exhibit the long-winged trait, as the v+ allele is dominant over v. This example beautifully demonstrates the separation of alleles during gamete formation and their subsequent combination to give rise to three distinct genotypes in the hybrids.
  • Morgan’s work with Drosophila not only provided practical evidence of Mendelian inheritance in action but also laid the foundation for understanding gene linkage and the concept of genetic mapping. His innovative research paved the way for the study of complex genetic phenomena and established Drosophila as a powerful model organism for genetic research.
Morgan’s work on Drosophila
Morgan’s work on Drosophila

‌Why is Mendel’s Law of Segregation defined as the purity law of gametes?

Mendel’s Law of Segregation, also known as the First Law of Mendel, is often referred to as the “purity law of gametes” because it describes how alleles for a single trait segregate (or separate) so that each gamete carries only one allele for each gene. Here’s a breakdown of why it’s called the purity law:

  1. Alleles and Homozygous/Heterozygous Conditions: Genes come in different versions called alleles. An organism can either have two of the same alleles (homozygous) or two different alleles (heterozygous) for a given gene.
  2. Formation of Gametes: During the formation of gametes (sperm and egg cells in animals, pollen and ovules in plants), the two alleles for each gene separate from each other. This process occurs during meiosis, a type of cell division that reduces the chromosome number by half.
  3. Purity of Gametes: As a result of this separation, each gamete receives only one allele for each gene. This ensures that gametes are “pure” in terms of their genetic information for a particular gene. For example, if an organism is heterozygous (Aa) for a gene, its gametes will either carry the A allele or the a allele, but not both.
  4. Fertilization: When two gametes fuse during fertilization, the resulting offspring will have two alleles for each gene—one from each parent. This restores the diploid number of chromosomes in the offspring.

Mendel derived this law from his experiments with pea plants. He observed that when he crossed plants with two different traits (e.g., yellow seeds vs. green seeds), the first generation (F1) showed only one of the traits (e.g., all yellow seeds). However, when he allowed the F1 generation to self-fertilize, the second generation (F2) showed a 3:1 ratio of the traits (e.g., 3 yellow seeds for every 1 green seed). This indicated that the alleles had separated during gamete formation in the F1 plants, leading to the reappearance of the green seed trait in the F2 generation.

In summary, Mendel’s Law of Segregation is termed the “purity law of gametes” because it explains how alleles separate during gamete formation, ensuring that each gamete carries only one allele for each gene, maintaining the “purity” of genetic information.


Mendelian traits have alleles that can be dominant or recessive, which are inherited from parents to offspring. In plants, the flower’s color is determined by the inherited allele. Each parent contributes one allele to the offspring. The combination of alleles in the offspring results from the union of two gametes’ chromosomes during fertilization. These chromosomes are randomly separated during gamete formation, with meiosis playing a role in the process.

Mendel’s Law of Segregation

Where does the law of segregation occur in meiosis?

The Law of Segregation is observed during the process of meiosis, specifically during the first division of meiosis, known as meiosis I. Here’s a breakdown of where and how the Law of Segregation occurs in meiosis:

  1. Prophase I: During this phase, homologous chromosomes (each consisting of two sister chromatids) pair up in a process called synapsis. This pairing forms structures called tetrads. While the Law of Segregation is not directly observed in this phase, the pairing of homologous chromosomes sets the stage for their separation in the next phases.
  2. Metaphase I: The tetrads align at the metaphase plate in the middle of the cell. The orientation of each homologous pair is random, which leads to genetic variation due to independent assortment. However, the Law of Segregation is still not directly observed here.
  3. Anaphase I: This is the critical phase where the Law of Segregation is observed. The homologous chromosomes are pulled apart and move to opposite poles of the cell. Importantly, each homologous chromosome carries one allele of a gene, ensuring that the two alleles of a gene present in a diploid cell are separated.
  4. Telophase I and Cytokinesis: The cell divides into two daughter cells, each with half the original chromosome number (haploid). Each daughter cell contains one chromosome (and therefore one allele) from each homologous pair.
  5. Meiosis II: This is the second division of meiosis, and it resembles mitosis. The sister chromatids of each chromosome are separated. However, the Law of Segregation primarily pertains to the separation of homologous chromosomes in meiosis I, not the separation of sister chromatids in meiosis II.

In summary, the Law of Segregation is most directly observed during anaphase I of meiosis, when the homologous chromosomes, each carrying one of the two alleles of a gene, are separated and move to opposite poles of the cell. This ensures that each resulting gamete contains only one allele for each gene.

Why is the Law of Segregation universally accepted?

The Law of Segregation is universally accepted for several reasons:

  1. Empirical Evidence: Gregor Mendel’s experiments with pea plants provided clear and repeatable evidence supporting the Law of Segregation. Mendel’s meticulous work and statistical analysis showed consistent patterns of inheritance that could be explained by the separation of alleles during gamete formation.
  2. Consistency Across Organisms: After Mendel’s findings were rediscovered at the turn of the 20th century, other researchers observed similar patterns of inheritance in various organisms, from fruit flies to humans. The consistency of these observations across diverse species reinforced the universality of the Law of Segregation.
  3. Molecular Biology Confirmation: With the advent of molecular biology and genetics in the 20th century, the physical basis of Mendel’s laws became clear. The structure and behavior of chromosomes during meiosis directly supported Mendel’s postulates. The discovery of the processes of DNA replication, transcription, and translation further solidified the understanding of genetic inheritance and confirmed Mendel’s findings at the molecular level.
  4. Predictive Power: The Law of Segregation, along with Mendel’s other laws, provides a robust framework for predicting the outcomes of genetic crosses. This predictive power has been utilized in various fields, from agriculture to medicine, to achieve desired genetic outcomes or understand genetic disorders.
  5. Integration with Other Genetic Principles: The Law of Segregation integrates seamlessly with other genetic principles and laws, such as the Law of Independent Assortment and the concept of linkage. This integration has allowed for a more comprehensive understanding of genetics and inheritance.
  6. Challenges and Refinements: While Mendel’s laws provide a foundational understanding of inheritance, they are not without exceptions. Over time, as exceptions and complexities (like linkage, incomplete dominance, and epistasis) were discovered, they didn’t invalidate the Law of Segregation. Instead, they added layers of complexity to our understanding of genetics. The fact that the law held true despite these refinements and challenges further solidified its acceptance.

In summary, the universal acceptance of the Law of Segregation is due to its empirical foundation, consistency across organisms, molecular confirmation, predictive power, and its ability to integrate with and withstand challenges from other genetic findings.

Limitations of Mendel’s Law of Segregation

While Mendel’s Law of Segregation stands as a cornerstone in genetics, there are certain limitations that must be acknowledged within its scope. These limitations include:

  1. Applicability to Diploid Organisms: The law is primarily relevant to diploid organisms, which are formed from haploid gametes during sexual reproduction. It doesn’t address scenarios involving haploid organisms or those that reproduce asexually.
  2. Incomplete Dominance or Codominance: The law does not hold true when dealing with alleles that display incomplete dominance or codominance. In such cases, the heterozygous condition results in a phenotype that is an intermediate blend of the two alleles or a distinct expression of both alleles, respectively.
  3. Limited to Gene Pairs with Dominant-Recessive Alleles: Mendel’s Law of Segregation is applicable only to traits governed by gene pairs where one allele is dominant over the other. It doesn’t account for more complex inheritance patterns or interactions.
  4. Non-Applicability to Collaborative Genes: The law is not valid for genes that collaborate or interact to influence a trait’s expression. It’s also unsuitable for genes that may exhibit variable expressions based on their interaction with other genes.
  5. Inadequate for Complementary Genes: Mendel’s law does not extend to traits determined by complementary gene pairs, where the presence of both dominant alleles is required for a specific phenotype to manifest.
  6. Not Suitable for Multifactorial Traits: The law is not applicable to traits that are governed by more than one gene pair. Many traits, such as height or skin color, are influenced by multiple genes, and Mendel’s law oversimplifies the inheritance of such traits.

In essence, while Mendel’s Law of Segregation provides a foundational understanding of genetic inheritance, it has limitations in explaining the complexities of gene interactions, multiple gene contributions, and non-dominant inheritance patterns. These limitations have driven further research and exploration in genetics to develop a more comprehensive understanding of inheritance and genetic variation.

Mendel’s Law of Segregation Mindmap

Mendel’s Law of Segregation Mindmap
Mendel’s Law of Segregation Mindmap

Mendel’s Law of Segregation Infograph

Mendel’s Law of Segregation Infograph
Mendel’s Law of Segregation Infograph
Download the Mendel’s Law of Segregation Infograph


What is Mendel’s Law of Segregation?

It is one of the fundamental principles of genetics that states that the two alleles for each gene segregate (separate) during gamete formation, and each gamete carries only one allele for each gene.

Who proposed the Law of Segregation?

The Law of Segregation was proposed by Gregor Mendel, a 19th-century Austrian monk and scientist, based on his experiments with pea plants.

How did Mendel discover this law?

Mendel discovered the law through his breeding experiments with pea plants, where he observed the inheritance patterns of single traits like seed color and flower color.

Why is it called the “Law of Segregation”?

It’s called the “Law of Segregation” because the two alleles for a gene segregate or separate from each other during the formation of gametes.

How does the Law of Segregation relate to meiosis?

During meiosis, the process by which gametes are formed, the paired alleles for each gene segregate so that each gamete receives only one allele.

What happens if an organism is heterozygous for a trait?

If an organism is heterozygous for a trait, it possesses two different alleles for that gene. According to the Law of Segregation, these alleles will separate during gamete formation.

How does the Law of Segregation explain genetic variation?

The law explains genetic variation by stating that offspring inherit a combination of alleles from both parents, leading to diverse genetic makeup.

Is the Law of Segregation always true?

While Mendel’s Law of Segregation applies to many genes, there are exceptions due to phenomena like linkage, where genes located close together on a chromosome tend to be inherited together.

How does the Law of Segregation differ from the Law of Independent Assortment?

The Law of Segregation deals with the separation of alleles for a single gene, while the Law of Independent Assortment refers to the independent inheritance of two or more genes located on different chromosomes.

Why is Mendel’s Law of Segregation important in genetics?

The law forms the foundation of genetic inheritance and provides a basic understanding of how traits are passed from one generation to the next, making it fundamental to the study of genetics.


  1. Genetics Generation. (2012). Genetics Generation.
  2. O’Neil, D. (2012). “Basic Principles of Genetics: Mendel’s Genetics.” Basic Principles of Genetics: Mendel’s Genetics.
  3. Hartwell, L. H., Goldberg, M. L., Fischer, J. A., & Hood, L. (2017). Genetics: From genes to the genome. Columbus: McGraw-Hill Higher Education.
  4. Pierce, B. A. (2017). Genetics: A conceptual approach. New York: W.H. Freeman. Snustad, D. P., & Simmons, M. J. (2015). Principles of genetics. New Jersey: Wiley.
  5. Watson, J. D., Baker, T. A., Stephen, P. B., Alexander, G., Michael, L., & Richard, L. (2013). Molecular biology of the gene. London: Pearson
  6. Bailey, Regina. “The 4 Concepts Related to Mendel’s Law of Segregation.” ThoughtCo. N.p., n.d. Web. Available here. 10 Aug. 2017. “Mendels Law of Independent Assortment – Boundless Open Textbook.” Boundless. N.p., 26 May 2016. Web. Available here. 10 Aug. 2017.
  7. An Overview on Law Of Segregation and Law Of Dominance. (2020), from

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