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Clostridium botulinum – Overview

Clostridium botulinum - Overview

Types of Botulism  There are three primary forms of botulism: baby, wound, and foodborne. Additional clinical classifications include adult intestinal toxaemia and iatrogenic botulism. 1. Foodborne botulism 2. Infant botulism 3. Wound botulism 4. Adult intestinal toxemi 5. Iatrogenic botulism Morphology of Clostridium botulinum C. botulinum has the following characteristics: Habitat of Clostridium botulinum Geographical … Read more

Clostridium tetani – Overview

Clostridium tetani - Overview

Tetanus is caused by C. tetani, an obligate anaerobic Gram-positive bacillus. Tetanus is an infectious disease characterised by increased muscle tone and spasms caused by the release of tetanospasmin, a neurotoxin produced by C. tetani when it is inoculated into humans. Morphology of Clostridium tetani C. tetani demonstrates the following characteristics: Geographical distribution of Clostridium … Read more

Clostridium perfringens – Overview

Clostridium perfringens

The genus Clostridium comprises Gram-positive, anaerobic, endospore-forming bacilli. The endospores are typically larger than the bacilli’s bodies, giving the bacteria a spindle-like appearance, hence the name Clostridium. Clostridium  In order to classify clostridia, numerous methods have been utilised. The standard approach for classifying Clostridium is predicated on a combination of the following characteristics: According to … Read more

Classification of Bacteria

Classification of Bacteria

Bacteria Definition Bacteria are prokaryotic, unicellular organisms without a real nucleus and a few organelles. Phenotypic Characteristics of Bacteria Morphologic Characteristics Growth Characteristics Antigens and Phage Susceptibility Biochemical Characteristics Reproduction in Bacteria Useful Bacteria Not all germs pose a threat to human health. There are bacteria that are advantageous in many ways. Below are a … Read more

Extremophiles – Definition, Classification, Examples

Extremophiles - Definition, Classification, Examples

Extremophiles Definition Extremophiles are organisms that have evolved to thrive in settings that were once believed to be completely inhospitable. These habitats are unfriendly, reaching extremes of severe heat, acidity, pressure, and cold that are lethal to most other forms of life. Due to the fact that extremophiles inhabit the extreme ends of the spectrum, … Read more

Asexual Reproduction in bacteria – Definition, Types, Advantages, Disadvantages

Asexual Reproduction in bacteria - Definition, Types, Advantages, Disadvantages

What is Asexual Reproduction? There are animals that are born with two parents. Some animals can come from a single parent. For instance, the Komodo dragon, as an instance is the only one with one mother and reproduces through parthenogenesis. Sexual reproduction is the process of generating offspring from only one parent. Are there male … Read more

Colony Morphology of Bacteria and Examples

Colony Morphology of Bacteria and Examples

Bacterial colonies are an essential element of microbiology that is relevant today and will likely remain the same. These colonies are utilized to conduct medical research, the development of pharmaceuticals, and use within the industry of food (the healthy ones, naturally) and many more. When a bacterial colony has been established, the first step to do is recognize it by the morphology of the bacterial colony it exhibits.

Staphylococcus aureus

Staphylococcus aureus

Domain: Bacteria Phylum: Bacillota Class: Bacilli Order: Bacillales Family: Staphylococcaceae Genus: Staphylococcus Species: S. aureus Overview of Staphylococcus aureus Staphylococcus aureus can be described as a gram-positive round-shaped bacterium that is one of the Firmicutes and is a typical member of the microbiota in the body. It is often located in the respiratory tract as … Read more

Cultivation of bacteria

Cultivation of bacteria

Bacteria constitute a vital element in the ecology. They are essential to our health as well as the environmental health, play an essential role in the production of food, and offer bioengineers the tools to harness their abilities and produce compounds. However, they could also be harmful, causing harm and diseases. The capacity to cultivate the microbes that cause harm is an essential element in being capable of harnessing their power, recognize the most harmful causes and improve our knowledge and capabilities. This article will look at the complexities of bacterial culture and the factors that affect conditions in culture as well as common issues and a few of the many applications.

L-form bacteria Definition, Size and shape, Culturing , Applications

L-form bacteria Definition, Size and shape, Culturing , Applications

“L-form” bacteria are also referred to as L-phase bacteria or L-phase variants, and cell wall deficient (CWD) bacteria are bacteria with no cell walls. They were first identified around 1935, by the scientist Emmy Klieneberger Nobel who identified them as “L-forms” after the Lister Institute in London which she was working at.

Bacterial Culture Technique

Bacterial Culture Technique

Microbiological cultures, also known as Microbial cultureis the technique of generating microbial species by the process of letting them reproduce in a conditions in a monitored laboratory conditions. Microbiological cultures are fundamental and essential diagnostic methods that are employed as research tools within molecular biology. The term”culture” could be used to describe the microorganisms that are being grown.

Protoplasts fusion – Somatic fusion

Protoplasts fusion - Somatic fusion

The process of somatic fusion is also known as protoplast Fusion, is a kind of genetic modification of plants in which two plant species are merged into a hybrid plant that has the traits of both species the species, an somatic hybrid. Hybrids are created among different species that are of the exact same species (e.g. between non-flowering potato plant species and flowers-producing potato plant) and between distinct species (e.g. among wheat Triticum as well as Rye Secale to make Triticale).

Protoplasts Isolation

Protoplasts Isolation

The protoplast, sometimes referred to as a naked plant cell refers to all the constituents in a cell of plant that are not the wall of the cell. The term”protoplast” was first used in the work of Hanstein in 1880 to refer to the living matter contained within the membrane of the plant cell. The protoplast that is isolated is unique because the plasma membrane’s outer layer is completely exposed and forms the only barrier between the outside surrounding environment and the inner living cells.

Protoplasts Definition, Application and Protoplasts Culture

Protoplasts Definition, Application and Protoplasts Culture

Protoplasts are plant cells that are completely naked with no cell wall however they do have plasma membranes and other components of the cell. They are functional cells, but without the presence of a barrier, cell wall. Protoplasts from various species can be merged to create a hybrid, and this is known as somatic hybridization (or protoplast fusion). Cybridization is the process of the fusion of protoplasts of normal size with nucleated (without nucleus) protoplast, resulting in the creation of a cybrid, also known as cytoplast (cytoplasmic hybrids).

Sphaeroplasts Definition, Formation, Applications

Sphaeroplasts Definition, Formation, Applications

A Spheroplast (or sphaeroplast according to British use) can be described as a microbe organism with a cell wall that is almost entirely gone through the penicillin or Lysozyme. According to certain definitions the term is employed to refer to Gram-negative bacteria. In other definitions, the word also includes yeasts. Spheroplast’s name is derived in the sense that once the cell wall of the microbe is digested by membrane tension, the cell to take on the characteristic shape of a spherical. Spheroplasts can be osmotically fragile and can lyse when transferred to a hypotonic environment.

Structure of Gram-negative cell wall

Structure of Gram-negative cell wall

Cell wall of the Gram-negative is more complicated than the Gram-positive cell wall. The amount of peptidoglycan present in the Gram-negative cell wall is considerably lower than that of that of the cell’s Gram positive wall. There are only a few layers of peptidoglycan (2-8 millimeters) are visible in the cell membrane’s outermost. A Gram-negative wall that lies outside the peptidoglycan layer has three primary components–(a) the lipoprotein layer, (b) outer membrane as well as (c) Lipopolysaccharides.

Structure of Gram-positive cell wall

Structure of Gram-positive cell wall

The cell’s outer envelope is an organelle and physiological protection between the protoplasm (inside) of the cell and its external environment. The cell envelope guards bacteria from osmotic destruction and gives bacteria a rigid form. The cell envelope is composed of two elements that are a cell wall as well as a cytoplasmic or plasma membrane. It is a protective layer for the protoplasm composed of (i) the cytoplasm, (ii) inclusions in the cytoplasm (mesosomes, ribosomes and vacuoles, inclusion granules) and (iii) one circular DNA.

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