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Nuclear Fast Red Solution Preparation and Application

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Table of Contents

This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.

Lakes are also formed with lead, ferric ion, copper, tin, and strontium, but not barium.1,2 The suitability of nuclear fast red as a nuclear stain in autoradiographs of knee joints of mice and rats has been reported.

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Nuclear Fast Red Solution Application

Used as a red nuclear counterstain. Nuclear Fast Red solution has been used:

  • In the dual-beam (FIB (focused ion beam)/SEM (scanning electron microscopy)) analysis of intracellular MNPs (magnetic nanoparticles) distribution.
  • As a counterstain in LacZ tissue histological analysis, and in extracellular matrix proteoglycan staining.
  • In situ hybridization.

Components Require

The following components are required to prepare 1L of Nuclear Fast Red Solution.

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ComponentAmountConcentration
nuclear fast red (Kernechtrot; C.I. 60760) (mw: 357.27 g/mol)1 g0.0028 M
aluminum sulfate (Al2[SO4]3•18H2O) (mw: 666.43 g/mol)50 g0.075 M

Nuclear Fast Red Solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of nuclear fast red (Kernechtrot; C.I. 60760) to the solution.
  3. Add 50 g of aluminum sulfate (Al2[SO4]3•18H2O) to the solution.
  4. Add distilled water until volume is 1 L.
  5. Heat with stirring until nearly boiling, then leave overnight to cool. There is a substantial residue of insoluble material, which must be removed by decanting and filtering. The solution keeps for ~1 yr; it may need to be filtered before each use.

References

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