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Ion exchange mechanism in Ion Exchange Chromatography

Ion Exchange Chromatography

Ion exchange chromatography (IEC) is a chromatographic technique that allows for the separation of ions and polar molecules based on their charge. It utilizes a stationary phase composed of an insoluble matrix with covalently bonded charged groups. These charged groups can attract and bind ions of the opposite charge present in the mobile phase, which … Read more

Sampling of Microorganisms From Soil

Sampling of Microorganisms From Soil

Exploring the microscopic world within soil requires precise techniques for the effective sampling of microorganisms, such as bacteria. These organisms play crucial roles in various ecological processes, including nutrient cycling, and understanding their presence and diversity is vital for both scientific research and environmental monitoring. Two widely recognized methods facilitate the direct observation of these … Read more

Sampling of Bacteria From Water

Sampling of Bacteria From Water

When examining water samples for bacterial content, direct microscopic observation often yields minimal visible bacteria due to their small size and low concentrations. To overcome this challenge and accurately assess the bacterial population in water, scientists employ the filtration technique. This method is pivotal for both quantifying bacteria per unit volume of water and analyzing … Read more

Sampling of Bacteria From Air

Sampling of Bacteria From Air

The process of collecting bacteria from the air for analysis involves several techniques, one of the simplest being the utilization of open Petri dishes filled with a growth medium. These dishes are exposed to the air, allowing airborne bacteria to settle and subsequently grow on the medium, which can then be examined to identify the … Read more

Determination of Phosphorus In Milk

Determination of Phosphorus In Milk

Phosphorus is an essential mineral found in many foods, including milk. It plays a critical role in various bodily functions such as the formation of bones and teeth, energy production, and the synthesis of RNA and DNA, which are crucial for the growth and repair of cells. In milk, phosphorus is naturally present and contributes … Read more

Determination of Magnesium In Milk

Determination of Magnesium In Milk

Magnesium is an essential mineral found in many foods, including milk. It plays a vital role in numerous bodily functions, such as muscle and nerve function, blood sugar control, and bone health. The magnesium content in milk contributes to its nutritional value, making milk a beneficial part of a balanced diet. The amount of magnesium … Read more

Determination of Calcium In Milk

Determination of Calcium In Milk

Milk is widely recognized as a significant source of calcium, an essential mineral that plays a crucial role in the development and maintenance of strong bones and teeth. This mineral is particularly important for infants and young children, whose rapidly growing bodies demand high levels of calcium to ensure proper bone formation and density. Calcium’s … Read more

Determination of Lactose In Milk by Lane-Eynon Method

Determination of Lactose In Milk by Lane-Eynon Method

What is Lane-Eynon Method? Principle of Lane-Eynon Method The Lane-Eynon method is a titrimetric procedure that quantifies reducing sugars in a solution through the utilization of Fehling’s solution as the primary reagent. This method operates on the fundamental chemistry of reduction-oxidation reactions, where Fehling’s solution, composed of copper sulfate and an alkaline solution of sodium … Read more

Parts of a Microscope and Their Functions

Parts of Microscope

What are Microscopes? Parts of a Microscope The microscope parts are divided into two main categories,  such as; Structural parts of a microscope and their functions Structural parts of a microscope Location Function Head (Body Tube) Situated at the microscope’s apex. Serves as a pivotal connector, linking the eyepiece to the objective lenses, ensuring a … Read more

Microscope Condenser – Types, Parts, Mechanism, Uses

Condenser of a Microscope

A microscope condenser is an integral optical component in the realm of microscopy, designed to focus and direct light onto the specimen being examined. This specialized lens system is strategically positioned beneath the microscope stage, a placement crucial for optimal illumination. The primary function of the condenser is to gather light from the microscope’s light … Read more

Objective Lenses – Principle, Types, Specification, Uses

Objective Lenses - Principle, Types, Specification, Uses

What is Objective Lens? Purpose of Objective Lenses Objective lenses serve several key purposes in optical instruments, playing a critical role in magnification, image formation, and resolution. The primary purposes of objective lenses include: Specification of Objective Lens The specification of an objective lens encompasses several key parameters that define its performance and suitability for … Read more

Folin-Wu Method For Estimation of Blood Glucose

Folin-Wu Method For Estimation of Blood Glucose

What is Folin-Wu Method? Principle of Folin-Wu Method Requirements Procedure of Folin-Wu Method Step 1: Preparation of Protein-Free Filtrate Step 2: Testing Procedure This process results in a color change proportional to the glucose concentration in the blood sample. By comparing the absorbance of the test sample against the standard, the glucose level in the … Read more

Sahli’s Method For The Estimation Of Hemoglobin

Sahli’s Method For The Estimation Of Hemoglobin

Hemoglobin, often abbreviated as Hb, is a vital protein found in red blood cells (RBCs). Its primary role in the human body includes the transport of oxygen from the lungs to various tissues and the transfer of carbon dioxide from tissues back to the lungs. Additionally, hemoglobin plays a significant role in maintaining the pH … Read more

What is Mantoux Test? – Procedure, Normal Range and Result Interpretation

Mantoux Test - Procedure, Normal Range and Result Interpretation

What is Mantoux Test? Principle of Mantoux Test The Mantoux test operates on the principle of delayed-type hypersensitivity, specifically Type IV hypersensitivity. This test is designed to assess a person’s cell-mediated immunity against the bacterium Mycobacterium tuberculosis. To conduct this test, a substance known as Purified Protein Derivative (PPD) is used, which is essentially a … Read more

Diaphragm of a Microscope – Definition, Types, Mechanism, Functions

Diaphragm of a Microscope - Definition, Types, Mechanism, Functions

What is Diaphragm of a Microscope? History of Diaphragm Definition of Diaphragm of a Microscope The diaphragm of a microscope is a component that controls the amount of light that passes through the specimen being observed. It is typically located between the light source and the stage, and consists of a series of adjustable blades … Read more

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