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Serial Dilution Method – Definition, Procedure, Application

Serial Dilutions and Plating Procedure

What is a serial dilution? Also Read: MCQ on Serial Dilution Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous … Read more

Subculturing technique for Broth Culture

Subculturing technique for Broth Culture

Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more

Subculturing Technique In Agar Slant/Agar Plate

Subculturing Technique

Overview of Subculturing Technique In microbiology, subculture is defined as a new cell or microbiological culture, which is prepared by transferring a few or all cells to a fresh growth medium from an old culture or a previous culture. In a single word, the transfer of cells from a previous culture to a fresh or … Read more

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

Pour Plate Method Principle, Procedure, Objective, Result

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species.  During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more

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