Elek test Principle, Procedure, Result
can be described as an test of immunoprecipitation (immunodiffusion) test that determines what specific strain of Corynebacterium Diphtheriae is toxic.
Practical Procedure
Get Study notes related to practicals of microbiology.
Topics Under This Category
Advertisements
SPONSOR AD
Potato Dextrose Agar (PDA) Principle, Composition, Preparation, Uses
Potato Dextrose Agar (PDA) is commonly abbreviated as PDA.
Potato Dextrose Agar has been suggested by APHA and F.D.A.for the count of moulds and yeasts during the testing of food items and dairy products.
Lowenstein Jensen (LJ) Media Composition, Preparation, Uses
Lowenstein Jensen Medium (LJ Medium) is a highly selective medium.
Solid media that are used to isolate as well as cultivation of Mycobacteria can be egg-based or Agar-based.
Carbohydrate Fermentation Test – Sugar Fermentation Test
The carbohydrate fermentation test can be used to determine if bacteria are able to ferment a certain carbohydrate.
It is a test to determine any presence of acids or gas resulting from carbohydrate fermentation.
Anaerobic Culture Media Examples, Definition
Anaerobic Culture Media used in anaerobic bacteriology may be prepared in a fresh manner or bought from commercial vendors.
Media for anaerobic fermentation that is created in the laboratory should be used within two weeks after the time of preparation, as prolonged storage can degrade the quality of the medium due to peroxide accumulation and dehydration.
Salmonella Shigella Agar (SS Agar) Principle, Composition, Application
Salmonella Shigella (SS) Agar can be moderately specific and a differing medium used for the cultivation, isolation as well as differentiation Salmonella spp. and a few varieties that belong to Shigella spp.
Modified Kirby-Bauer Disc Diffusion Method
Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory.
Mueller Hinton Agar (MHA) Composition, Principle, Preparation
What is Mueller Hinton Agar (MHA)? Why MHA is used for antibiotic susceptibility testing (AST)? Mueller Hinton Agar (MHA) is commonly used for antibiotic susceptibility testing due to several key reasons: In summary, MHA is used for antibiotic susceptibility testing due to its non-selective nature, starch content for toxin absorption and controlled diffusion, loose agar … Read more
MacConkey Agar – Composition, Uses, Colony Characteristics
MacConkey Agar is a selective and differentiated culture medium widely used to isolate of Gram-negative enteric bacteria.
It is selective since it allows for the growth of gram-negative bacteria , and differentiating the bacteria that are gram-negative based on their metabolic process of lactose.
KOH Test (Potassium hydroxide test) Principle, Procedure, Results, Uses
A KOH pre-test is a quick, non-invasive procedure to diagnose fungal infections that affect the nails or skin.
The cells are collected from the affected region and put on a slide using an acid composed of potassium hydroxide. They are looked at under a microscope in order to determine if there are evidence of the presence of a mold.
Bile Esculin Test Principle, Procedure, Result, and application.
Bile Esculin test The Bile Esculin test is a biological test that is used to distinguish Enterococci from Group D Streptococci from non-group D viridans group Streptococci by their capacity to hydrolyze esculin. A variety of organisms are capable hydrolyzing esculin. But only a handful of them can do it when they are surrounded by … Read more
Quantitative Buffy Coat Test (QBC Test)
Two different methods of blood tests, thin and thick microscopy, are considered to be the “gold standard” for the diagnosis of malaria. A Quantitative Buffy Coat(QBC) is an additional quick and direct test to make the identification of malaria.
Sheep blood agar Composition, Principle, Procedure, Uses.
Sheep blood agar Sheep Blood Agar is a very nutritious medium that is used in growing and isolating various kinds of microorganisms. The Sheep Blood Agar made up of the Oxoid formulation. The formulated medium is believed to have an improved nutritional value, resulting in a higher growth rate and a larger colony size, as … Read more
Blood Agar Definition, Preparation, Composition, Application, and limitation
production of blood agar after the addition of liquid blood.
Arneth Count for Neutrophils – Principle, Procedure.
The Arneth Count determines the percentage distribution of various types of neutrophils based on their nuclear lobes. It isn’t used often in modern medicine. Joseph Arneth divided neutrophils into five types. Stage Nuclear lobes Diagram Normal Range Stage 1 (N1) Nucleus is unilobed.The nucleus is C or U shaped. 2-5% Stage 2 (N2) Nucleus is … Read more
Solubility Tests of Proteins Principle, Procedure, Result, Application
Solubility Tests is a type of biochemical test which is performed to check the ability of compounds to dissolve within a liquid solvent.
Biuret Test For Protein Principle, Procedure, Result, Application.
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.
Alkaline Peptone Water Preparation, Composition, Principle, Application.
Clinical materials containing small numbers of Vibrio should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar (M189). Alkaline Peptone Water is a suitable enrichment broth for this purpose.
Peptone Water – Principle, Composition, Preparation, Uses
Peptone Water is a type of broth medium. This media is mainly used to grow the organism and is also used as a base for determining carbohydrate fermentation patterns of non-fastidious organisms. It is also used to detect indole production by the organism.
Tollens’ Test Definition, Principle, Procedure, Result, Application.
It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.
