Northern Blot Protocol, Principle, Application, Result
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Northern Blotting Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Giolitti-Cantoni Broth for Staphylococcus aureus
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Giolitti-Cantoni Broth for Staphylococcus aureus

Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.

Measurement Of Microorganisms - Micrometry
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Measurement Of Microorganisms – Micrometry

A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10x45, which is 450 times larger than the standard one. We don't know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.

Gel Staining Procedure for PAGE
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Gel Staining Procedure for PAGE

Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.

Modified Kirby-Bauer Disc Diffusion Method
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Modified Kirby-Bauer Disc Diffusion Method

Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory. 

Xanthoproteic test Principle, Procedure, Result, Application
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Xanthoproteic test Principle, Procedure, Result, Application

Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc. The presence of proteins can be confirmed qualitatively by usign several tests, Xanthoproteic test is one of them. Due to the presence of characteristic side chains in them, certain amino acids exhibit typical color reactions that form the basis for their identification.

Blood Grouping Principle and Procedure
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Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Polymerase chain reaction (PCR)
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Polymerase Chain Reaction (PCR) Steps, Principle, Application

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).

Buffer preparation
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Eastern blot Protocol, Principle, Application

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

Western Blot Protocol, Principle, Result
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Western Blot Protocol, Principle, Result.

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.

Restriction Digest Principle, Procedure, result
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Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

Methylene Blue Reduction Test
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Methylene Blue Reduction Test

Methylene Blue Reduction Test also known as mbrt test. It is a qualitative test for milk, it used to check

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