Solution preparation is an important skill in the laboratory, as it involves the accurate measurement and mixing of chemicals to create a desired solution. Here are some general steps for preparing a laboratory solution:
It is important to follow proper laboratory safety guidelines when preparing solutions, including wearing protective equipment, such as goggles and gloves, and following any specific handling instructions for the chemicals being used. It is also important to accurately measure and record the amount of solute and solvent used, as this information is critical for reproducing the solution and for accurately calculating the concentration of the solute in the solution.
SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic
Tris-SDS Buffer (pH 6.8) is used to prepare buffer for stacking gel during SDS-PAGE (SDSPolyacrylamide gel electrophoresis). It is a discontinuous gel with a huge pores size to concentrate proteins prior to entering the separating zone. The buffer is sold in a 5X stock.
This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.
ACK Lysis Buffer Preparation ACK Lysis Buffer is used to lyse red blood cells. To prepare 1L of ACK Lysis
Tyrode’s Solution, Acidic Preparation To prepare 1L of Tyrode’s Solution, Acidic, the following components are required; Component Amount Concentration NaCl
pH Buffer solution A pH buffer or hydrogen ion buffer is defined as an aqueous solution that is composed of
Acrylamide Stock Solution Preparation Application: Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide
Chelation Medium Preparation To prepare 1L of Chelation Medium the following components are required. Component Amount Concentration NaCl (mw: 58.44
Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited
Calcium Chloride Stock Solution Preparation Calcium chloride is a common calcium salt used in a variety of applications. To prepare
Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH
Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.
This “Mayer’s hemalum solution - for microscopy” is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin.
Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.
Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols.
Cresol red is used as a tracking dye for DNA/RNA in agarose gel electrophoresis and protein in polyacrylamide gel electrophoresis.
It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.
This solution is mainly used to determine the concentration of proteins in solution.
Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.
