A light microscope may be used to see and examine the vacuole's structure. While the vacuole doesn't stain as the other organelles of the cell (because it does not contain many constituents that stain) tests have demonstrated that staining is possible for this organelle because the vacuole's sap absorbs and stores dyes that are colored.
Different stains have been developed over time to distinguish bacteria species, separating them morphologically and the specific characteristics they possess. The most popular stain is Gram staining, acid-fast staining, and endospore staining. Each stain aims at identifying and defining bacteria according to their forms and morphologies.
Different stains react or concentrate on different areas of a tissue or cell These properties can be utilized to highlight certain areas or regions. A few of the most well-known biological staining methods can be found below. If not otherwise indicated All of these dyes can be used on tissues and cells that are fixed as well as essential dyes (suitable for use in live organisms) are indicated.
Wright’s stain is a modified Romanowsky stain. In hematology laboratory, it is used for the staining of peripheral blood smear, bone marrow aspirates, and urine samples.
The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with
Flow cytometry is a fast and stable method for the quantification of viable cells. Determining cell viability is a significant
Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within
The composition of cell wall varies from species to species, it has been reported that the main constituents of cell wall is chitin, hemicellulose, and cellulose. In addition, prokaryotic cell wall contains peptidoglycan (also known as murein and mucopeptide). Peptidoglycan is mainly composed of sugar, amino acids (peptide; amino acids + glycan; sugar).
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.