Amoeba - Fixing, Staining Techniques
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Staining

Amoeba Staining, Fixing, Techniques

Amoeba (less often spelled ameba, or amoeba, plural am(o)ebas or am(o)ebae) is often referred to as an amoeboid, is kind

Vacuole Staining - Observation of Vacuoles under Microscope
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Vacuole Staining – Observation of Vacuoles under Microscope

A light microscope may be used to see and examine the vacuole's structure. While the vacuole doesn't stain as the other organelles of the cell (because it does not contain many constituents that stain) tests have demonstrated that staining is possible for this organelle because the vacuole's sap absorbs and stores dyes that are colored.

Papanicolaou Staining (Pap stain)
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Papanicolaou Staining (Pap stain)

Papanicolaou stain can also be referred to as the pap stain, and the process that causes the stain is referred to as a pap smear.

Albert stain Principle, Procedure, Result
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Albert Stain Principle, Procedure, Result

Different stains have been developed over time to distinguish bacteria species, separating them morphologically and the specific characteristics they possess. The most popular stain is Gram staining, acid-fast staining, and endospore staining. Each stain aims at identifying and defining bacteria according to their forms and morphologies.

Types of Stains used in Microbiology
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Types of Stains used in Microbiology

Different stains react or concentrate on different areas of a tissue or cell These properties can be utilized to highlight certain areas or regions. A few of the most well-known biological staining methods can be found below. If not otherwise indicated All of these dyes can be used on tissues and cells that are fixed as well as essential dyes (suitable for use in live organisms) are indicated.

Types of Staining Techniques
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Types of Staining Techniques

Staining is a method used to boost the contrast of samples, usually at the microscopic scale.

Wright Stain Principle, Procedure, Result
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Wright Stain Principle, Procedure, Result

Wright’s stain is a modified Romanowsky stain. In hematology laboratory, it is used for the staining of peripheral blood smear, bone marrow aspirates, and urine samples.

Nuclear Staining of Filamentous Fungi
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Nuclear Staining of Filamentous Fungi

Fungi are eukaryotic and contain well-organized nuclei. The fungal nucleus is membrane bound and consist of two membranes of characteristic

Viability Staining Method For Bacteria
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Viability Staining Method For Bacteria

Aim To distinguish between living and dead cells. Principle The viability Staining Method mainly used to detect living and dead

Bacterial cell wall structure
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Cell Wall Staining by Dayr’s Method

The composition of cell wall varies from species to species, it has been reported that the main constituents of cell wall is chitin, hemicellulose, and cellulose. In addition, prokaryotic cell wall contains peptidoglycan (also known as murein and mucopeptide). Peptidoglycan is mainly composed of sugar, amino acids (peptide; amino acids + glycan; sugar).

Collagen Hybridizing Peptide Staining
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Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.

Acridine Orange Staining
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Acridine Orange Staining

Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.

Toluidine blue stain of Mast cell
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Toluidine blue stain of Mast Cell.

Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).

Sudan Black B Staining
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Sudan Black B Staining

Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.