Solution preparation

Preparation of Crystal Violet for the Gram Stain

This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

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This article writter by MN Editors on November 22, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Preparation of Crystal Violet for the Gram Stain
Preparation of Crystal Violet for the Gram Stain

Preparation of Crystal Violet (Recipe for Crystal Violet)

This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

Ingredients of Crystal Violet

IngredientsAmount
crystal violet2 g
95% ethyl alcohol20 ml
ammonium citrate monohydrate0.8 g
deionized water80 ml

Preparation Steps of Crystal Violet Stain

  1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
  2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
  3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.
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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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