Preparation of Crystal Violet for the Gram Stain

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Preparation of Crystal Violet (Recipe for Crystal Violet)

This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

Ingredients of Crystal Violet

crystal violet2 g
95% ethyl alcohol20 ml
ammonium citrate monohydrate0.8 g
deionized water80 ml

Preparation Steps of Crystal Violet Stain

  1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
  2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
  3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.


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