Protein Concentration Calculator (OD260/OD280)
Instruction
- Unknown pure proteins or protein mixtures: Use the following formula to roughly estimate protein concentration. Protein Concentration (mg/ml) = OD280 divided by cuvette width (cm)
- Pure protein of known absorbance coefficient. Protein concentration = OD280 divided by (absorbance coefficient * cuvette width)
- Unknown protein with possible nucleic acid contamination. Use the following formula to estimate protein concentration: Protein Concentration (mg/ml) = (1.55 * OD280) – (0.76 * OD260)
