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Solution preparation

Pyronin Y (PY) Solution Preparation and Application

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and...

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This article writter by SouravBio on June 02, 2021

Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

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Buffer preparation
Buffer preparation

Pyronin Y (PY) Solution Application

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

Components Require

The following components are required to prepare 1L of Pyronin Y (PY) Solution.

ComponentAmountConcentration
Pyronin Y(G) (PY) (Polysciences, Inc.) (mw: 302.8 g/mol)1 g0.0033 M

Pyronin Y (PY) Solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of Pyronin Y(G) (PY) (Polysciences, Inc.) to the solution.
  3. Add distilled water until volume is 1 L.
  4. Store at 4°C protected from light.

References

“Pyronin Y (PY) Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pyronin-y-g-py-stock-solution-1-mg-ml.

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Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

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