Solution preparation

Pyronin Y (PY) Solution Preparation and Application

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and...

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This article writter by MN Editors on June 02, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Buffer preparation
Buffer preparation

Pyronin Y (PY) Solution Application

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

Components Require

The following components are required to prepare 1L of Pyronin Y (PY) Solution.

ComponentAmountConcentration
Pyronin Y(G) (PY) (Polysciences, Inc.) (mw: 302.8 g/mol)1 g0.0033 M

Pyronin Y (PY) Solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of Pyronin Y(G) (PY) (Polysciences, Inc.) to the solution.
  3. Add distilled water until volume is 1 L.
  4. Store at 4°C protected from light.

References

“Pyronin Y (PY) Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pyronin-y-g-py-stock-solution-1-mg-ml.

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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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