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When produced by recombinant DNA technology in E. Coli, somatostatin is initially attached to

A. Latex beads

B. A fragment of b-galactosidase

C. The plasma membrane

D. The recombinant plasmid containing its gene

1 Answer

B. A fragment of β-galactosidase.

When somatostatin is produced by recombinant DNA technology in E. coli, it is initially attached to a fragment of β-galactosidase.

In recombinant DNA technology, the gene encoding somatostatin is inserted into a plasmid vector, which is then introduced into E. coli cells for expression. To facilitate the identification and purification of the expressed somatostatin, a common approach is to fuse it with a reporter protein, such as a fragment of β-galactosidase.

The fusion protein consists of the somatostatin peptide sequence linked to a portion of β-galactosidase. This allows the detection and isolation of cells that are producing the desired somatostatin peptide by using colorimetric or other assays that detect the activity or presence of β-galactosidase.

By fusing somatostatin to a fragment of β-galactosidase, the expressed somatostatin can be easily identified and separated from other cellular components during the purification process.

Therefore, the correct answer is B. A fragment of β-galactosidase.
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