Question

Students of Class XII visited Microbial Type Culture Collection, Chandigarh and observed microbial cultures of Providencia stuartii, Streptomyces albus and Haemophilus aegyptus. Name the restriction enzymes obtained from them and also specify their restriction sites.

Answer 1

It is not possible to determine the restriction enzymes and their specific recognition sites from the names of the microorganisms alone. The restriction enzymes and their recognition sites can only be determined by conducting experiments using the genomic DNA of the microorganisms and testing the DNA with various restriction enzymes.

Additionally, it is important to note that not all microorganisms will produce restriction enzymes, and even those that do may produce different types of enzymes with varying recognition sites. Therefore, it is necessary to identify the specific strains of the microorganisms and conduct experiments to determine the restriction enzymes and their recognition sites.