Question

A sample of protein, 9 x 10-12 moles has to be analysed for its amino acid sequence. Which technique/s can be used for the analysis?  

A. 2 D Gel Electrophoresis  

B. Protein fingerprinting  

C. Mass Spectrometry  

D. Both (a) and (b)

Answer 1

Option C - Mass Spectrometry can be used to analyze the amino acid sequence of a protein sample.

Mass Spectrometry is a powerful analytical technique that can provide information about the mass, charge, and structural characteristics of molecules, including proteins. It can be used to determine the molecular weight of a protein and identify its constituent amino acids. This technique involves ionizing the protein molecules, separating them based on their mass-to-charge ratio, and detecting the ions produced. The resulting mass spectra can be analyzed to determine the amino acid sequence of the protein.

Option A - 2D Gel Electrophoresis and Option B - Protein fingerprinting can be used to separate and identify proteins in a sample but may not be able to provide information on the amino acid sequence of a protein.