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pUC19 and a linear DNA (both of same size) have two sites for the same restriction - endonuclease. When cleaved and separated on agarose gel electrophoresis, how many bands will be visualized in each case and why?

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When pUC19 and a linear DNA (both of same size) are cleaved by a restriction endonuclease with two recognition sites, the number of bands visualized on an agarose gel electrophoresis depends on the location of the recognition sites on the DNA molecule.

If both recognition sites are present on the same fragment of DNA, then there will be two fragments of different sizes in the case of both pUC19 and linear DNA. Thus, two bands will be visualized in each case.

If the recognition sites are present on different fragments of DNA, then there will be three fragments of different sizes. In the case of pUC19, two fragments will be present, one containing both recognition sites, and the other without any recognition site. In the case of linear DNA, three fragments will be present, one containing both recognition sites, one containing only one recognition site, and one without any recognition site.
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