Question

Set up below is an IEF gel for the separation of four proteins A, B, C and D obtained in a cell extract. The pH of the proteins A to D is 5.5, 6, 7 and 8 respectively. Study the set up and answer the questions that follow: 

(a) Which proteins A to D can be separated using the above setup? 

(b) What change in the above set up is required in order to separate all the proteins?

Answer 1

(a) Protein samples A and B will get separated using this set up. (b) Using ampholytes with broader range covering pH value range from 3 to 11 will be able to isolate all the four proteins.