Restriction Enzymes Cut Sites

Restriction Enzymes Cut Sites

EnzymeRecognition SequenceCutOverhang
AatII5'- G A C G T/C -'3
3'- C/T G C A G -'5
5'-GACGT      C-3'
3'-C      TGCAG-5'
3' ACGT
AbaSI5'- C N N N N N N N N N N N/N N N N N N N N N G -'3
3'- G N N N N N N N N N/N N N N N N N N N N N C -'5
5'-CNNNNNNNNNNN      NNNNNNNNNG-3'
3'-GNNNNNNNNN      NNNNNNNNNNNC-5'
3' NN
Acc65I5'- G/G T A C C -'3
3'- C C A T G/G -'5
5'-G      GTACC-3'
3'-CCATG      G-5'
5' GTAC
AccI5'- G T/M K A C -'3
3'- C A K M/T G -'5
5'-GT      MKAC-3'
3'-CAKM      TG-5'
5' MK
AciI5'- C/C G C -'3
3'- G G C/G -'5
5'-C      CGC-3'
3'-GGC      G-5'
5' CG
AclI5'- A A/C G T T -'3
3'- T T G C/A A -'5
5'-AA      CGTT-3'
3'-TTGC      AA-5'
5' CG
AcuI5'- C T G A A G N14 N N/-'3
3'- G A C T T C N14/N N -'5
5'-CTGAAGN14NN      -3'
3'-GACTTCN14      NN-5'
3' NN
AfeI5'- A G C/G C T -'3
3'- T C G/C G A -'5
5'-AGC      GCT-3'
3'-TCG      CGA-5'
Blunt
AflII5'- C/T T A A G -'3
3'- G A A T T/C -'5
5'-C      TTAAG-3'
3'-GAATT      C-5'
5' TTAA
AflIII5'- A/C R Y G T -'3
3'- T G Y R C/A -'5
5'-A      CRYGT-3'
3'-TGYRC      A-5'
5' CRYG
AgeI5'- A/C C G G T -'3
3'- T G G C C/A -'5
5'-A      CCGGT-3'
3'-TGGCC      A-5'
5' CCGG
AhdI5'- G A C N N N/N N G T C -'3
3'- C T G N N/N N N C A G -'5
5'-GACNNN      NNGTC-3'
3'-CTGNN      NNNCAG-5'
3' N
AleI-v25'- C A C N N/N N G T G -'3
3'- G T G N N/N N C A C -'5
5'-CACNN      NNGTG-3'
3'-GTGNN      NNCAC-5'
Blunt
AluI5'- A G/C T -'3
3'- T C/G A -'5
5'-AG      CT-3'
3'-TC      GA-5'
Blunt
AlwI5'- G G A T C N4/N -'3
3'- C C T A G N4 N/-'5
5'-GGATCN4      N-3'
3'-CCTAGN4N      -5'
5' N
AlwNI5'- C A G N N N/C T G -'3
3'- G T C/N N N G A C -'5
5'-CAGNNN      CTG-3'
3'-GTC      NNNGAC-5'
3' NNN
ApaI5'- G G G C C/C -'3
3'- C/C C G G G -'5
5'-GGGCC      C-3'
3'-C      CCGGG-5'
3' GGCC
ApaLI5'- G/T G C A C -'3
3'- C A C G T/G -'5
5'-G      TGCAC-3'
3'-CACGT      G-5'
5' TGCA
ApoI5'- R/A A T T Y -'3
3'- Y T T A A/R -'5
5'-R      AATTY-3'
3'-YTTAA      R-5'
5' AATT
AscI5'- G G/C G C G C C -'3
3'- C C G C G C/G G -'5
5'-GG      CGCGCC-3'
3'-CCGCGC      GG-5'
5' CGCG
AseI5'- A T/T A A T -'3
3'- T A A T/T A -'5
5'-AT      TAAT-3'
3'-TAAT      TA-5'
5' TA
AsiSI5'- G C G A T/C G C -'3
3'- C G C/T A G C G -'5
5'-GCGAT      CGC-3'
3'-CGC      TAGCG-5'
3' AT
AvaI BsoBI5'- C/Y C G R G -'3
3'- G R G C Y/C -'5
5'-C      YCGRG-3'
3'-GRGCY      C-5'
5' YCGR
AvaII5'- G/G W C C -'3
3'- C C W G/G -'5
5'-G      GWCC-3'
3'-CCWG      G-5'
5' GWC
AvrII5'- C/C T A G G -'3
3'- G G A T C/C -'5
5'-C      CTAGG-3'
3'-GGATC      C-5'
5' CTAG
BaeGI5'- G K G C M/C -'3
3'- C/M C G K G -'5
5'-GKGCM      C-3'
3'-C      MCGKG-5'
3' KGCM
BaeI5'- N N N N N/N10 A C N N N N G T A Y C N7 N N N N N/-'3
3'-/N N N N N N10 T G N N N N C A T R G N7/N N N N N -'5
5'-NNNNN      N10ACNNNNGTAYCN7NNNNN      -3'
3'-      NNNNNN10TGNNNNCATRGN7      NNNNN-5'
3' NNNNN
BamHI5'- G/G A T C C -'3
3'- C C T A G/G -'5
5'-G      GATCC-3'
3'-CCTAG      G-5'
5' GATC
BanI5'- G/G Y R C C -'3
3'- C C R Y G/G -'5
5'-G      GYRCC-3'
3'-CCRYG      G-5'
5' GYRC
BanII5'- G R G C Y/C -'3
3'- C/Y C G R G -'5
5'-GRGCY      C-3'
3'-C      YCGRG-5'
3' RGCY
BbsI5'- G A A G A C N2/N N N N -'3
3'- C T T C T G N2 N N N N/-'5
5'-GAAGACN2      NNNN-3'
3'-CTTCTGN2NNNN      -5'
5' NNNN
BbvCI5'- C C/T C A G C -'3
3'- G G A G T/C G -'5
5'-CC      TCAGC-3'
3'-GGAGT      CG-5'
5' TCA
BbvI5'- G C A G C N8/N N N N -'3
3'- C G T C G N8 N N N N/-'5
5'-GCAGCN8      NNNN-3'
3'-CGTCGN8NNNN      -5'
5' NNNN
BccI5'- C C A T C N4/N -'3
3'- G G T A G N4 N/-'5
5'-CCATCN4      N-3'
3'-GGTAGN4N      -5'
5' N
BceAI5'- A C G G C N12/N N -'3
3'- T G C C G N12 N N/-'5
5'-ACGGCN12      NN-3'
3'-TGCCGN12NN      -5'
5' NN
BcgI5'- N N/N10 C G A N N N N N N T G C N10 N N/-'3
3'-/N N N10 G C T N N N N N N A C G N10/N N -'5
5'-NN      N10CGANNNNNNTGCN10NN      -3'
3'-      NNN10GCTNNNNNNACGN10      NN-5'
3' NN
BciVI5'- G T A T C C N5 N/-'3
3'- C A T A G G N5/N -'5
5'-GTATCCN5N      -3'
3'-CATAGGN5      N-5'
3' N
BclI5'- T/G A T C A -'3
3'- A C T A G/T -'5
5'-T      GATCA-3'
3'-ACTAG      T-5'
5' GATC
BfaI5'- C/T A G -'3
3'- G A T/C -'5
5'-C      TAG-3'
3'-GAT      C-5'
5' TA
BglI5'- G C C N N N N/N G G C -'3
3'- C G G N/N N N N C C G -'5
5'-GCCNNNN      NGGC-3'
3'-CGGN      NNNNCCG-5'
3' NNN
BglII5'- A/G A T C T -'3
3'- T C T A G/A -'5
5'-A      GATCT-3'
3'-TCTAG      A-5'
5' GATC
BlpI5'- G C/T N A G C -'3
3'- C G A N T/C G -'5
5'-GC      TNAGC-3'
3'-CGANT      CG-5'
5' TNA
BmgBI5'- C A C/G T C -'3
3'- G T G/C A G -'5
5'-CAC      GTC-3'
3'-GTG      CAG-5'
Blunt
BmrI5'- A C T G G G N4 N/-'3
3'- T G A C C C N4/N -'5
5'-ACTGGGN4N      -3'
3'-TGACCCN4      N-5'
3' N
BmtI5'- G C T A G/C -'3
3'- C/G A T C G -'5
5'-GCTAG      C-3'
3'-C      GATCG-5'
3' CTAG
BpmI5'- C T G G A G N14 N N/-'3
3'- G A C C T C N14/N N -'5
5'-CTGGAGN14NN      -3'
3'-GACCTCN14      NN-5'
3' NN
Bpu10I5'- C C/T N A G C -'3
3'- G G A N T/C G -'5
5'-CC      TNAGC-3'
3'-GGANT      CG-5'
5' TNA
BpuEI5'- C T T G A G N14 N N/-'3
3'- G A A C T C N14/N N -'5
5'-CTTGAGN14NN      -3'
3'-GAACTCN14      NN-5'
3' NN
BsaAI5'- Y A C/G T R -'3
3'- R T G/C A Y -'5
5'-YAC      GTR-3'
3'-RTG      CAY-5'
Blunt
BsaBI5'- G A T N N/N N A T C -'3
3'- C T A N N/N N T A G -'5
5'-GATNN      NNATC-3'
3'-CTANN      NNTAG-5'
Blunt
BsaHI5'- G R/C G Y C -'3
3'- C Y G C/R G -'5
5'-GR      CGYC-3'
3'-CYGC      RG-5'
5' CG
BsaI5'- G G T C T C N/N N N N -'3
3'- C C A G A G N N N N N/-'5
5'-GGTCTCN      NNNN-3'
3'-CCAGAGNNNNN      -5'
5' NNNN
BsaJI5'- C/C N N G G -'3
3'- G G N N C/C -'5
5'-C      CNNGG-3'
3'-GGNNC      C-5'
5' CNNG
BsaWI5'- W/C C G G W -'3
3'- W G G C C/W -'5
5'-W      CCGGW-3'
3'-WGGCC      W-5'
5' CCGG
BsaXI5'- N N N/N9 A C N N N N N C T C C N7 N N N/-'3
3'-/N N N N9 T G N N N N N G A G G N7/N N N -'5
5'-NNN      N9ACNNNNNCTCCN7NNN      -3'
3'-      NNNN9TGNNNNNGAGGN7      NNN-5'
3' NNN
BseRI5'- G A G G A G N8 N N/-'3
3'- C T C C T C N8/N N -'5
5'-GAGGAGN8NN      -3'
3'-CTCCTCN8      NN-5'
3' NN
BseYI5'- C/C C A G C -'3
3'- G G G T C/G -'5
5'-C      CCAGC-3'
3'-GGGTC      G-5'
5' CCAG
BsgI5'- G T G C A G N14 N N/-'3
3'- C A C G T C N14/N N -'5
5'-GTGCAGN14NN      -3'
3'-CACGTCN14      NN-5'
3' NN
BsiEI5'- C G R Y/C G -'3
3'- G C/Y R G C -'5
5'-CGRY      CG-3'
3'-GC      YRGC-5'
3' RY
BsiHKAI5'- G W G C W/C -'3
3'- C/W C G W G -'5
5'-GWGCW      C-3'
3'-C      WCGWG-5'
3' WGCW
BsiWI5'- C/G T A C G -'3
3'- G C A T G/C -'5
5'-C      GTACG-3'
3'-GCATG      C-5'
5' GTAC
BslI5'- C C N N N N N/N N G G -'3
3'- G G N N/N N N N N C C -'5
5'-CCNNNNN      NNGG-3'
3'-GGNN      NNNNNCC-5'
3' NNN
BsmAI BcoDI5'- G T C T C N/N N N N -'3
3'- C A G A G N N N N N/-'5
5'-GTCTCN      NNNN-3'
3'-CAGAGNNNNN      -5'
5' NNNN
BsmBI-v25'- C G T C T C N/N N N N -'3
3'- G C A G A G N N N N N/-'5
5'-CGTCTCN      NNNN-3'
3'-GCAGAGNNNNN      -5'
5' NNNN
BsmFI5'- G G G A C N10/N N N N -'3
3'- C C C T G N10 N N N N/-'5
5'-GGGACN10      NNNN-3'
3'-CCCTGN10NNNN      -5'
5' NNNN
BsmI5'- G A A T G C N/-'3
3'- C T T A C/G N -'5
5'-GAATGCN      -3'
3'-CTTAC      GN-5'
3' CN
Bsp1286I5'- G D G C H/C -'3
3'- C/H C G D G -'5
5'-GDGCH      C-3'
3'-C      HCGDG-5'
3' DGCH
BspCNI5'- C T C A G N7 N N/-'3
3'- G A G T C N7/N N -'5
5'-CTCAGN7NN      -3'
3'-GAGTCN7      NN-5'
3' NN
BspEI5'- T/C C G G A -'3
3'- A G G C C/T -'5
5'-T      CCGGA-3'
3'-AGGCC      T-5'
5' CCGG
BspHI5'- T/C A T G A -'3
3'- A G T A C/T -'5
5'-T      CATGA-3'
3'-AGTAC      T-5'
5' CATG
BspMI BfuAI5'- A C C T G C N4/N N N N -'3
3'- T G G A C G N4 N N N N/-'5
5'-ACCTGCN4      NNNN-3'
3'-TGGACGN4NNNN      -5'
5' NNNN
BsrBI5'- C C G/C T C -'3
3'- G G C/G A G -'5
5'-CCG      CTC-3'
3'-GGC      GAG-5'
Blunt
BsrDI5'- G C A A T G N N/-'3
3'- C G T T A C/N N -'5
5'-GCAATGNN      -3'
3'-CGTTAC      NN-5'
3' NN
BsrFI-v25'- R/C C G G Y -'3
3'- Y G G C C/R -'5
5'-R      CCGGY-3'
3'-YGGCC      R-5'
5' CCGG
BsrGI5'- T/G T A C A -'3
3'- A C A T G/T -'5
5'-T      GTACA-3'
3'-ACATG      T-5'
5' GTAC
BsrI5'- A C T G G N/-'3
3'- T G A C/C N -'5
5'-ACTGGN      -3'
3'-TGAC      CN-5'
3' GN
BssHII5'- G/C G C G C -'3
3'- C G C G C/G -'5
5'-G      CGCGC-3'
3'-CGCGC      G-5'
5' CGCG
BssSI-v25'- C/A C G A G -'3
3'- G T G C T/C -'5
5'-C      ACGAG-3'
3'-GTGCT      C-5'
5' ACGA
BstAPI5'- G C A N N N N/N T G C -'3
3'- C G T N/N N N N A C G -'5
5'-GCANNNN      NTGC-3'
3'-CGTN      NNNNACG-5'
3' NNN
BstBI5'- T T/C G A A -'3
3'- A A G C/T T -'5
5'-TT      CGAA-3'
3'-AAGC      TT-5'
5' CG
BstEII BstEII5'- G/G T N A C C -'3
3'- C C A N T G/G -'5
5'-G      GTNACC-3'
3'-CCANTG      G-5'
5' GTNAC
BstNI5'- C C/W G G -'3
3'- G G W/C C -'5
5'-CC      WGG-3'
3'-GGW      CC-5'
5' W
BstUI5'- C G/C G -'3
3'- G C/G C -'5
5'-CG      CG-3'
3'-GC      GC-5'
Blunt
BstXI5'- C C A N N N N N/N T G G -'3
3'- G G T N/N N N N N A C C -'5
5'-CCANNNNN      NTGG-3'
3'-GGTN      NNNNNACC-5'
3' NNNN
BstYI5'- R/G A T C Y -'3
3'- Y C T A G/R -'5
5'-R      GATCY-3'
3'-YCTAG      R-5'
5' GATC
BstZ17I5'- G T A/T A C -'3
3'- C A T/A T G -'5
5'-GTA      TAC-3'
3'-CAT      ATG-5'
Blunt
Bsu36I5'- C C/T N A G G -'3
3'- G G A N T/C C -'5
5'-CC      TNAGG-3'
3'-GGANT      CC-5'
5' TNA
BtgI5'- C/C R Y G G -'3
3'- G G Y R C/C -'5
5'-C      CRYGG-3'
3'-GGYRC      C-5'
5' CRYG
BtgZI5'- G C G A T G N10/N N N N -'3
3'- C G C T A C N10 N N N N/-'5
5'-GCGATGN10      NNNN-3'
3'-CGCTACN10NNNN      -5'
5' NNNN
BtsCI5'- G G A T G N N/-'3
3'- C C T A C/N N -'5
5'-GGATGNN      -3'
3'-CCTAC      NN-5'
3' NN
BtsI-v25'- G C A G T G N N/-'3
3'- C G T C A C/N N -'5
5'-GCAGTGNN      -3'
3'-CGTCAC      NN-5'
3' NN
BtsIMutI5'- C A G T G N N/-'3
3'- G T C A C/N N -'5
5'-CAGTGNN      -3'
3'-GTCAC      NN-5'
3' NN
Cac8I5'- G C N/N G C -'3
3'- C G N/N C G -'5
5'-GCN      NGC-3'
3'-CGN      NCG-5'
Blunt
ClaI BspDI5'- A T/C G A T -'3
3'- T A G C/T A -'5
5'-AT      CGAT-3'
3'-TAGC      TA-5'
5' CG
CspCI5'- N N/N11 C A A N N N N N G T G G N10 N N/-'3
3'-/N N N11 G T T N N N N N C A C C N10/N N -'5
5'-NN      N11CAANNNNNGTGGN10NN      -3'
3'-      NNN11GTTNNNNNCACCN10      NN-5'
3' NN
CviAII5'- C/A T G -'3
3'- G T A/C -'5
5'-C      ATG-3'
3'-GTA      C-5'
5' AT
CviKI-15'- R G/C Y -'3
3'- Y C/G R -'5
5'-RG      CY-3'
3'-YC      GR-5'
Blunt
CviQI5'- G/T A C -'3
3'- C A T/G -'5
5'-G      TAC-3'
3'-CAT      G-5'
5' TA
DdeI5'- C/T N A G -'3
3'- G A N T/C -'5
5'-C      TNAG-3'
3'-GANT      C-5'
5' TNA
DpnI5'- G A/T C -'3
3'- C T/A G -'5
5'-GA      TC-3'
3'-CT      AG-5'
Blunt
DraI5'- T T T/A A A -'3
3'- A A A/T T T -'5
5'-TTT      AAA-3'
3'-AAA      TTT-5'
Blunt
DraIII5'- C A C N N N/G T G -'3
3'- G T G/N N N C A C -'5
5'-CACNNN      GTG-3'
3'-GTG      NNNCAC-5'
3' NNN
DrdI5'- G A C N N N N/N N G T C -'3
3'- C T G N N/N N N N C A G -'5
5'-GACNNNN      NNGTC-3'
3'-CTGNN      NNNNCAG-5'
3' NN
EaeI5'- Y/G G C C R -'3
3'- R C C G G/Y -'5
5'-Y      GGCCR-3'
3'-RCCGG      Y-5'
5' GGCC
EagI5'- C/G G C C G -'3
3'- G C C G G/C -'5
5'-C      GGCCG-3'
3'-GCCGG      C-5'
5' GGCC
EarI5'- C T C T T C N/N N N -'3
3'- G A G A A G N N N N/-'5
5'-CTCTTCN      NNN-3'
3'-GAGAAGNNNN      -5'
5' NNN
EciI5'- G G C G G A N9 N N/-'3
3'- C C G C C T N9/N N -'5
5'-GGCGGAN9NN      -3'
3'-CCGCCTN9      NN-5'
3' NN
Eco53kI5'- G A G/C T C -'3
3'- C T C/G A G -'5
5'-GAG      CTC-3'
3'-CTC      GAG-5'
Blunt
EcoNI5'- C C T N N/N N N A G G -'3
3'- G G A N N N/N N T C C -'5
5'-CCTNN      NNNAGG-3'
3'-GGANNN      NNTCC-5'
5' N
EcoO109I5'- R G/G N C C Y -'3
3'- Y C C N G/G R -'5
5'-RG      GNCCY-3'
3'-YCCNG      GR-5'
5' GNC
EcoP15I5'- C A G C A G N25/N N -'3
3'- G T C G T C N25 N N/-'5
5'-CAGCAGN25      NN-3'
3'-GTCGTCN25NN      -5'
5' NN
EcoRI5'- G/A A T T C -'3
3'- C T T A A/G -'5
5'-G      AATTC-3'
3'-CTTAA      G-5'
5' AATT
EcoRV5'- G A T/A T C -'3
3'- C T A/T A G -'5
5'-GAT      ATC-3'
3'-CTA      TAG-5'
Blunt
Esp3I5'- C G T C T C N/N N N N -'3
3'- G C A G A G N N N N N/-'5
5'-CGTCTCN      NNNN-3'
3'-GCAGAGNNNNN      -5'
5' NNNN
FatI5'-/C A T G -'3
3'- G T A C/-'5
5'-      CATG-3'
3'-GTAC      -5'
5' CATG
FauI5'- C C C G C N4/N N -'3
3'- G G G C G N4 N N/-'5
5'-CCCGCN4      NN-3'
3'-GGGCGN4NN      -5'
5' NN
Fnu4HI5'- G C/N G C -'3
3'- C G N/C G -'5
5'-GC      NGC-3'
3'-CGN      CG-5'
5' N
FokI5'- G G A T G N9/N N N N -'3
3'- C C T A C N9 N N N N/-'5
5'-GGATGN9      NNNN-3'
3'-CCTACN9NNNN      -5'
5' NNNN
FseI5'- G G C C G G/C C -'3
3'- C C/G G C C G G -'5
5'-GGCCGG      CC-3'
3'-CC      GGCCGG-5'
3' CCGG
FspEI5'- C C N12/N N N N -'3
3'- G G N12 N N N N/-'5
5'-CCN12      NNNN-3'
3'-GGN12NNNN      -5'
5' NNNN
FspI5'- T G C/G C A -'3
3'- A C G/C G T -'5
5'-TGC      GCA-3'
3'-ACG      CGT-5'
Blunt
HaeII5'- R G C G C/Y -'3
3'- Y/C G C G R -'5
5'-RGCGC      Y-3'
3'-Y      CGCGR-5'
3' GCGC
HaeIII5'- G G/C C -'3
3'- C C/G G -'5
5'-GG      CC-3'
3'-CC      GG-5'
Blunt
HgaI5'- G A C G C N5/N N N N N -'3
3'- C T G C G N5 N N N N N/-'5
5'-GACGCN5      NNNNN-3'
3'-CTGCGN5NNNNN      -5'
5' NNNNN
HhaI5'- G C G/C -'3
3'- C/G C G -'5
5'-GCG      C-3'
3'-C      GCG-5'
3' CG
HincII5'- G T Y/R A C -'3
3'- C A R/Y T G -'5
5'-GTY      RAC-3'
3'-CAR      YTG-5'
Blunt
HindIII5'- A/A G C T T -'3
3'- T T C G A/A -'5
5'-A      AGCTT-3'
3'-TTCGA      A-5'
5' AGCT
HinfI5'- G/A N T C -'3
3'- C T N A/G -'5
5'-G      ANTC-3'
3'-CTNA      G-5'
5' ANT
HinP1I5'- G/C G C -'3
3'- C G C/G -'5
5'-G      CGC-3'
3'-CGC      G-5'
5' CG
HpaI5'- G T T/A A C -'3
3'- C A A/T T G -'5
5'-GTT      AAC-3'
3'-CAA      TTG-5'
Blunt
HphI5'- G G T G A N7 N/-'3
3'- C C A C T N7/N -'5
5'-GGTGAN7N      -3'
3'-CCACTN7      N-5'
3' N
Hpy166II5'- G T N/N A C -'3
3'- C A N/N T G -'5
5'-GTN      NAC-3'
3'-CAN      NTG-5'
Blunt
Hpy188I5'- T C N/G A -'3
3'- A G/N C T -'5
5'-TCN      GA-3'
3'-AG      NCT-5'
3' N
Hpy188III5'- T C/N N G A -'3
3'- A G N N/C T -'5
5'-TC      NNGA-3'
3'-AGNN      CT-5'
5' NN
Hpy99I5'- C G W C G/-'3
3'-/G C W G C -'5
5'-CGWCG      -3'
3'-      GCWGC-5'
3' CGWCG
HpyAV5'- C C T T C N5 N/-'3
3'- G G A A G N5/N -'5
5'-CCTTCN5N      -3'
3'-GGAAGN5      N-5'
3' N
HpyCH4III5'- A C N/G T -'3
3'- T G/N C A -'5
5'-ACN      GT-3'
3'-TG      NCA-5'
3' N
HpyCH4IV5'- A/C G T -'3
3'- T G C/A -'5
5'-A      CGT-3'
3'-TGC      A-5'
5' CG
HpyCH4V5'- T G/C A -'3
3'- A C/G T -'5
5'-TG      CA-3'
3'-AC      GT-5'
Blunt
I-CeuI5'- T A A C T A T A A C G G T C C T A A/G G T A G C G A A -'3
3'- A T T G A T A T T G C C A G/G A T T C C A T C G C T T -'5
5'-TAACTATAACGGTCCTAA      GGTAGCGAA-3'
3'-ATTGATATTGCCAG      GATTCCATCGCTT-5'
3' CTAA
I-SceI5'- T A G G G A T A A/C A G G G T A A T -'3
3'- A T C C C/T A T T G T C C C A T T A -'5
5'-TAGGGATAA      CAGGGTAAT-3'
3'-ATCCC      TATTGTCCCATTA-5'
3' ATAA
KasI5'- G/G C G C C -'3
3'- C C G C G/G -'5
5'-G      GCGCC-3'
3'-CCGCG      G-5'
5' GCGC
KpnI5'- G G T A C/C -'3
3'- C/C A T G G -'5
5'-GGTAC      C-3'
3'-C      CATGG-5'
3' GTAC
LpnPI5'- C C D G N10/N N N N -'3
3'- G G H C N10 N N N N/-'5
5'-CCDGN10      NNNN-3'
3'-GGHCN10NNNN      -5'
5' NNNN
MboI Sau3AI DpnII5'-/G A T C -'3
3'- C T A G/-'5
5'-      GATC-3'
3'-CTAG      -5'
5' GATC
MboII5'- G A A G A N7 N/-'3
3'- C T T C T N7/N -'5
5'-GAAGAN7N      -3'
3'-CTTCTN7      N-5'
3' N
MfeI5'- C/A A T T G -'3
3'- G T T A A/C -'5
5'-C      AATTG-3'
3'-GTTAA      C-5'
5' AATT
MluCI5'-/A A T T -'3
3'- T T A A/-'5
5'-      AATT-3'
3'-TTAA      -5'
5' AATT
MluI5'- A/C G C G T -'3
3'- T G C G C/A -'5
5'-A      CGCGT-3'
3'-TGCGC      A-5'
5' CGCG
MlyI5'- G A G T C N5/-'3
3'- C T C A G N5/-'5
5'-GAGTCN5      -3'
3'-CTCAGN5      -5'
Blunt
MmeI5'- T C C R A C N18 N N/-'3
3'- A G G Y T G N18/N N -'5
5'-TCCRACN18NN      -3'
3'-AGGYTGN18      NN-5'
3' NN
MnlI5'- C C T C N6 N/-'3
3'- G G A G N6/N -'5
5'-CCTCN6N      -3'
3'-GGAGN6      N-5'
3' N
MscI5'- T G G/C C A -'3
3'- A C C/G G T -'5
5'-TGG      CCA-3'
3'-ACC      GGT-5'
Blunt
MseI5'- T/T A A -'3
3'- A A T/T -'5
5'-T      TAA-3'
3'-AAT      T-5'
5' TA
MslI5'- C A Y N N/N N R T G -'3
3'- G T R N N/N N Y A C -'5
5'-CAYNN      NNRTG-3'
3'-GTRNN      NNYAC-5'
Blunt
MspA1I5'- C M G/C K G -'3
3'- G K C/G M C -'5
5'-CMG      CKG-3'
3'-GKC      GMC-5'
Blunt
MspI HpaII5'- C/C G G -'3
3'- G G C/C -'5
5'-C      CGG-3'
3'-GGC      C-5'
5' CG
MspJI5'- C N N R N9/N N N N -'3
3'- G N N Y N9 N N N N/-'5
5'-CNNRN9      NNNN-3'
3'-GNNYN9NNNN      -5'
5' NNNN
MwoI5'- G C N N N N N/N N G C -'3
3'- C G N N/N N N N N C G -'5
5'-GCNNNNN      NNGC-3'
3'-CGNN      NNNNNCG-5'
3' NNN
NaeI5'- G C C/G G C -'3
3'- C G G/C C G -'5
5'-GCC      GGC-3'
3'-CGG      CCG-5'
Blunt
NarI5'- G G/C G C C -'3
3'- C C G C/G G -'5
5'-GG      CGCC-3'
3'-CCGC      GG-5'
5' CG
NciI5'- C C/S G G -'3
3'- G G S/C C -'5
5'-CC      SGG-3'
3'-GGS      CC-5'
5' S
NcoI5'- C/C A T G G -'3
3'- G G T A C/C -'5
5'-C      CATGG-3'
3'-GGTAC      C-5'
5' CATG
NdeI5'- C A/T A T G -'3
3'- G T A T/A C -'5
5'-CA      TATG-3'
3'-GTAT      AC-5'
5' TA
NgoMIV5'- G/C C G G C -'3
3'- C G G C C/G -'5
5'-G      CCGGC-3'
3'-CGGCC      G-5'
5' CCGG
NheI5'- G/C T A G C -'3
3'- C G A T C/G -'5
5'-G      CTAGC-3'
3'-CGATC      G-5'
5' CTAG
NlaIII5'- C A T G/-'3
3'-/G T A C -'5
5'-CATG      -3'
3'-      GTAC-5'
3' CATG
NlaIV5'- G G N/N C C -'3
3'- C C N/N G G -'5
5'-GGN      NCC-3'
3'-CCN      NGG-5'
Blunt
NmeAIII5'- G C C G A G N19 N N/-'3
3'- C G G C T C N19/N N -'5
5'-GCCGAGN19NN      -3'
3'-CGGCTCN19      NN-5'
3' NN
NotI5'- G C/G G C C G C -'3
3'- C G C C G G/C G -'5
5'-GC      GGCCGC-3'
3'-CGCCGG      CG-5'
5' GGCC
NruI5'- T C G/C G A -'3
3'- A G C/G C T -'5
5'-TCG      CGA-3'
3'-AGC      GCT-5'
Blunt
NsiI5'- A T G C A/T -'3
3'- T/A C G T A -'5
5'-ATGCA      T-3'
3'-T      ACGTA-5'
3' TGCA
NspI5'- R C A T G/Y -'3
3'- Y/G T A C R -'5
5'-RCATG      Y-3'
3'-Y      GTACR-5'
3' CATG
Nt.AlwI5'- G G A T C N N N N/-'3
3'-/C C T A G N N N N -'5
5'-GGATCNNNN      -3'
3'-      CCTAGNNNN-5'
3' GGATCNNNN
Nt.BbvCI5'- C C/T C A G C -'3
3'-/G G A G T C G -'5
5'-CC      TCAGC-3'
3'-      GGAGTCG-5'
3' CC
Nt.BsmAI5'- G T C T C N/-'3
3'-/C A G A G N -'5
5'-GTCTCN      -3'
3'-      CAGAGN-5'
3' GTCTCN
Nt.BspQI5'- G C T C T T C N/-'3
3'-/C G A G A A G N -'5
5'-GCTCTTCN      -3'
3'-      CGAGAAGN-5'
3' GCTCTTCN
Nt.BstNBI5'- G A G T C N N N N/-'3
3'-/C T C A G N N N N -'5
5'-GAGTCNNNN      -3'
3'-      CTCAGNNNN-5'
3' GAGTCNNNN
PacI5'- T T A A T/T A A -'3
3'- A A T/T A A T T -'5
5'-TTAAT      TAA-3'
3'-AAT      TAATT-5'
3' AT
PciI5'- A/C A T G T -'3
3'- T G T A C/A -'5
5'-A      CATGT-3'
3'-TGTAC      A-5'
5' CATG
PflMI5'- C C A N N N N/N T G G -'3
3'- G G T N/N N N N A C C -'5
5'-CCANNNN      NTGG-3'
3'-GGTN      NNNNACC-5'
3' NNN
PI-PspI5'- T G G C A A A C A G C T A T T A T/G G G T A T T A T G G G T -'3
3'- A C C G T T T G T C G A T/A A T A C C C A T A A T A C C C A -'5
5'-TGGCAAACAGCTATTAT      GGGTATTATGGGT-3'
3'-ACCGTTTGTCGAT      AATACCCATAATACCCA-5'
3' TTAT
PI-SceI5'- A T C T A T G T C G G G T G C/G G A G A A A G A G G T A A T -'3
3'- T A G A T A C A G C C/C A C G C C T C T T T C T C C A T T A -'5
5'-ATCTATGTCGGGTGC      GGAGAAAGAGGTAAT-3'
3'-TAGATACAGCC      CACGCCTCTTTCTCCATTA-5'
3' GTGC
PleI5'- G A G T C N4/N -'3
3'- C T C A G N4 N/-'5
5'-GAGTCN4      N-3'
3'-CTCAGN4N      -5'
5' N
PluTI5'- G G C G C/C -'3
3'- C/C G C G G -'5
5'-GGCGC      C-3'
3'-C      CGCGG-5'
3' GCGC
PmeI5'- G T T T/A A A C -'3
3'- C A A A/T T T G -'5
5'-GTTT      AAAC-3'
3'-CAAA      TTTG-5'
Blunt
PmlI5'- C A C/G T G -'3
3'- G T G/C A C -'5
5'-CAC      GTG-3'
3'-GTG      CAC-5'
Blunt
PpuMI5'- R G/G W C C Y -'3
3'- Y C C W G/G R -'5
5'-RG      GWCCY-3'
3'-YCCWG      GR-5'
5' GWC
PshAI5'- G A C N N/N N G T C -'3
3'- C T G N N/N N C A G -'5
5'-GACNN      NNGTC-3'
3'-CTGNN      NNCAG-5'
Blunt
PsiI-v25'- T T A/T A A -'3
3'- A A T/A T T -'5
5'-TTA      TAA-3'
3'-AAT      ATT-5'
Blunt
PspGI5'-/C C W G G -'3
3'- G G W C C/-'5
5'-      CCWGG-3'
3'-GGWCC      -5'
5' CCWGG
PspOMI5'- G/G G C C C -'3
3'- C C C G G/G -'5
5'-G      GGCCC-3'
3'-CCCGG      G-5'
5' GGCC
PspXI5'- V C/T C G A G B -'3
3'- B G A G C T/C V -'5
5'-VC      TCGAGB-3'
3'-BGAGCT      CV-5'
5' TCGA
PstI5'- C T G C A/G -'3
3'- G/A C G T C -'5
5'-CTGCA      G-3'
3'-G      ACGTC-5'
3' TGCA
PvuI5'- C G A T/C G -'3
3'- G C/T A G C -'5
5'-CGAT      CG-3'
3'-GC      TAGC-5'
3' AT
PvuII5'- C A G/C T G -'3
3'- G T C/G A C -'5
5'-CAG      CTG-3'
3'-GTC      GAC-5'
Blunt
RsaI5'- G T/A C -'3
3'- C A/T G -'5
5'-GT      AC-3'
3'-CA      TG-5'
Blunt
RsrII5'- C G/G W C C G -'3
3'- G C C W G/G C -'5
5'-CG      GWCCG-3'
3'-GCCWG      GC-5'
5' GWC
SacI5'- G A G C T/C -'3
3'- C/T C G A G -'5
5'-GAGCT      C-3'
3'-C      TCGAG-5'
3' AGCT
SacII5'- C C G C/G G -'3
3'- G G/C G C C -'5
5'-CCGC      GG-3'
3'-GG      CGCC-5'
3' GC
SalI5'- G/T C G A C -'3
3'- C A G C T/G -'5
5'-G      TCGAC-3'
3'-CAGCT      G-5'
5' TCGA
SapI BspQI5'- G C T C T T C N/N N N -'3
3'- C G A G A A G N N N N/-'5
5'-GCTCTTCN      NNN-3'
3'-CGAGAAGNNNN      -5'
5' NNN
Sau96I5'- G/G N C C -'3
3'- C C N G/G -'5
5'-G      GNCC-3'
3'-CCNG      G-5'
5' GNC
SbfI5'- C C T G C A/G G -'3
3'- G G/A C G T C C -'5
5'-CCTGCA      GG-3'
3'-GG      ACGTCC-5'
3' TGCA
ScaI5'- A G T/A C T -'3
3'- T C A/T G A -'5
5'-AGT      ACT-3'
3'-TCA      TGA-5'
Blunt
ScrFI5'- C C/N G G -'3
3'- G G N/C C -'5
5'-CC      NGG-3'
3'-GGN      CC-5'
5' N
SexAI5'- A/C C W G G T -'3
3'- T G G W C C/A -'5
5'-A      CCWGGT-3'
3'-TGGWCC      A-5'
5' CCWGG
SfaNI5'- G C A T C N5/N N N N -'3
3'- C G T A G N5 N N N N/-'5
5'-GCATCN5      NNNN-3'
3'-CGTAGN5NNNN      -5'
5' NNNN
SfcI5'- C/T R Y A G -'3
3'- G A Y R T/C -'5
5'-C      TRYAG-3'
3'-GAYRT      C-5'
5' TRYA
SfiI5'- G G C C N N N N/N G G C C -'3
3'- C C G G N/N N N N C C G G -'5
5'-GGCCNNNN      NGGCC-3'
3'-CCGGN      NNNNCCGG-5'
3' NNN
SfoI5'- G G C/G C C -'3
3'- C C G/C G G -'5
5'-GGC      GCC-3'
3'-CCG      CGG-5'
Blunt
SgrAI5'- C R/C C G G Y G -'3
3'- G Y G G C C/R C -'5
5'-CR      CCGGYG-3'
3'-GYGGCC      RC-5'
5' CCGG
SmaI5'- C C C/G G G -'3
3'- G G G/C C C -'5
5'-CCC      GGG-3'
3'-GGG      CCC-5'
Blunt
SmlI5'- C/T Y R A G -'3
3'- G A R Y T/C -'5
5'-C      TYRAG-3'
3'-GARYT      C-5'
5' TYRA
SnaBI5'- T A C/G T A -'3
3'- A T G/C A T -'5
5'-TAC      GTA-3'
3'-ATG      CAT-5'
Blunt
SpeI5'- A/C T A G T -'3
3'- T G A T C/A -'5
5'-A      CTAGT-3'
3'-TGATC      A-5'
5' CTAG
SphI5'- G C A T G/C -'3
3'- C/G T A C G -'5
5'-GCATG      C-3'
3'-C      GTACG-5'
3' CATG
SrfI5'- G C C C/G G G C -'3
3'- C G G G/C C C G -'5
5'-GCCC      GGGC-3'
3'-CGGG      CCCG-5'
Blunt
SspI5'- A A T/A T T -'3
3'- T T A/T A A -'5
5'-AAT      ATT-3'
3'-TTA      TAA-5'
Blunt
StuI5'- A G G/C C T -'3
3'- T C C/G G A -'5
5'-AGG      CCT-3'
3'-TCC      GGA-5'
Blunt
StyD4I5'-/C C N G G -'3
3'- G G N C C/-'5
5'-      CCNGG-3'
3'-GGNCC      -5'
5' CCNGG
StyI5'- C/C W W G G -'3
3'- G G W W C/C -'5
5'-C      CWWGG-3'
3'-GGWWC      C-5'
5' CWWG
SwaI5'- A T T T/A A A T -'3
3'- T A A A/T T T A -'5
5'-ATTT      AAAT-3'
3'-TAAA      TTTA-5'
Blunt
TaqI-v25'- T/C G A -'3
3'- A G C/T -'5
5'-T      CGA-3'
3'-AGC      T-5'
5' CG
TfiI5'- G/A W T C -'3
3'- C T W A/G -'5
5'-G      AWTC-3'
3'-CTWA      G-5'
5' AWT
TseI ApeKI5'- G/C W G C -'3
3'- C G W C/G -'5
5'-G      CWGC-3'
3'-CGWC      G-5'
5' CWG
Tsp45I5'-/G T S A C -'3
3'- C A S T G/-'5
5'-      GTSAC-3'
3'-CASTG      -5'
5' GTSAC
TspRI5'- N N C A S T G N N/-'3
3'-/N N G T S A C N N -'5
5'-NNCASTGNN      -3'
3'-      NNGTSACNN-5'
3' NNCASTGNN
Tth111I PflFI5'- G A C N/N N G T C -'3
3'- C T G N N/N C A G -'5
5'-GACN      NNGTC-3'
3'-CTGNN      NCAG-5'
5' N
XbaI5'- T/C T A G A -'3
3'- A G A T C/T -'5
5'-T      CTAGA-3'
3'-AGATC      T-5'
5' CTAG
XcmI5'- C C A N N N N N/N N N N T G G -'3
3'- G G T N N N N/N N N N N A C C -'5
5'-CCANNNNN      NNNNTGG-3'
3'-GGTNNNN      NNNNNACC-5'
3' N
XhoI PaeR7I5'- C/T C G A G -'3
3'- G A G C T/C -'5
5'-C      TCGAG-3'
3'-GAGCT      C-5'
5' TCGA
XmaI TspMI5'- C/C C G G G -'3
3'- G G G C C/C -'5
5'-C      CCGGG-3'
3'-GGGCC      C-5'
5' CCGG
XmnI5'- G A A N N/N N T T C -'3
3'- C T T N N/N N A A G -'5
5'-GAANN      NNTTC-3'
3'-CTTNN      NNAAG-5'
Blunt
ZraI5'- G A C/G T C -'3
3'- C T G/C A G -'5
5'-GAC      GTC-3'
3'-CTG      CAG-5'
Blunt
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