AdBlock Detected

It looks like you're using an ad-blocker!

Our team work realy hard to produce quality content on this website and we noticed you have ad-blocking enabled.

X

Salt Meat Broth

By
MN Editors

Aim

Salt Meat Broth is used as an enrichment medium for staphylococci isolation from severely contaminated specimens.

Principle

Salt Meat Broth, an enrichment media used to isolate halophilic staphylococci in contaminated samples like faeces and blood. This is especially useful in cases of food poisoning. It is sensitive because it can detect low levels of staphylococci even in samples with high heterogeneous microbial populations. Because of high levels of sodium chloride, the medium is highly selective for staphylococci. Staphylococcus Aureus can tolerate high levels of sodium chloride, which inhibits most bacteria. Salt Meat Broth is also useful for cultivating some halophilic micrococci that are associated with raw salt and hides. This medium is not suitable for testing for coagulase production. Therefore, they should first be subcultured on Blood Agar. The essential nutrients for bacterial metabolism are provided by the peptic digest of animal tissue, and beef extract. Sodium chloride helps maintain osmotic equilibrium.

Salt Meat Broth Composition

Ingredients Gms / Litre
Beef extract 10
Peptic digest of animal tissue 10
Neutral ox-heart tissue 30
Sodium chloride 100
Final pH ( at 25°C) 7.6±0.2

Salt Meat Broth Preparation

  1. Take 15 grams and mix it with 100 ml of distilled water.
  2. Allow to soak for five minutes.
  3. If necessary, heat the medium to dissolve it completely.
  4. Use a test tube measuring 5/8 inches in diameter to disperse and dispense the mixture.
  5. For 15 minutes, sterilize by using an autoclave at 15 lbs pressure (121degC).

Procedure for Staphylococcus aureus isolation

  1. After incubation at 35deg-37OC for 24 to 48 hours, discrete colonies can be obtained by plating a small amount of the liquid culture onto Mannitol Salt Agar CM85 and Staphylococcus Medium.

Important

  • This medium is not recommended for enrichment of foods with staphylococci. If stressed organisms are not first incubated with a non-selective medium (e.g., broth), productivity is likely to suffer. To allow for resuscitation, you can use Soya broth made from Tryptone Soya.
  • This medium can be used to cultivate some of the halophilic micrococci that are associated with raw salt and hides.
  • It is important to note that staphylococci grown on this medium can’t be tested for coagulase activity. They should first be subcultured in a medium with less salt. This is why Blood Agar Base (CM55) is recommended.

 Storage Conditions and Shelf Life

  • Keep the dehydrated medium at 25°C, and use it before the expiry date printed on the label.
  • Keep the prepared medium at 25°C

Quality Control

  • Positive control: Staphylococcus aureus ATCC” 25923
  • Negative control: Escherichia coli ATCC” 25922

We hope you've enjoyed reading our latest blog article! We're thrilled to see the positive response it's been receiving so far. We understand that sometimes, after going through an interesting piece of content, you might have questions or want to delve deeper into the topic.

To facilitate meaningful discussions and encourage knowledge sharing, we've set up a dedicated QNA Forum page related to this specific article. If you have any questions, comments, or thoughts you'd like to share, we invite you to visit the QNA Forum.

QNA Forum Page

Feel free to ask your questions or participate in ongoing discussions. Our team of experts, as well as fellow readers, will be active on the forum to engage with you and provide insightful answers. Remember, sharing your thoughts not only helps you gain a deeper understanding but also contributes to the community's growth and learning. We look forward to hearing from you and fostering an enriching discussion. Thank you for being a part of our journey!

Leave a Comment