Solution preparation

SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g...

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This article writter by MN Editors on September 02, 2022

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SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe
SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

Required components

ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol)27.6 g0.2 M
EDTA (mw: 292.24 g/mol)7.4 g0.02 M

SSPE (Blot, 20x, pH 7.4) Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
  4. Add 7.4 g of EDTA to the solution.
  5. Add distilled water until the volume is 1 L.
  6. Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.

SSC (Northern Blot, 20x) Preparation and Recipe

Required components

ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Trisodium citrate (mw: 258.07 g/mol)88.3 g0.3 M

SSC (Northern Blot, 20x) Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 88.3 g of Trisodium citrate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Adjust the pH to 7.0 with NaOH or HCl. Autoclave the solution and store it at room temperature. It will last for 1 yr or longer.
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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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