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SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

By
MN Editors

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

Required components

Component Amount Concentration
Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g 0.2 M
EDTA (mw: 292.24 g/mol) 7.4 g 0.02 M

SSPE (Blot, 20x, pH 7.4) Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
  4. Add 7.4 g of EDTA to the solution.
  5. Add distilled water until the volume is 1 L.
  6. Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.

SSC (Northern Blot, 20x) Preparation and Recipe

Required components

Component Amount Concentration
Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M
Trisodium citrate (mw: 258.07 g/mol) 88.3 g 0.3 M

SSC (Northern Blot, 20x) Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 88.3 g of Trisodium citrate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Adjust the pH to 7.0 with NaOH or HCl. Autoclave the solution and store it at room temperature. It will last for 1 yr or longer.

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