Chromatography Archives – Microbiology Notes

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Gel Permeation Chromatography – Definition, Principle, Parts, Steps, Applications

Published on June 26, 2023 by MN Editors

Gel permeation chromatography may also be known as gel size exclusion or filtration. For size-exclusion chromatography it is the case that stationary phases are a porous matrices made consisting of substances like cross-linked polystyrene (CLP), polyacrylamide gels, cross-linked dextrans as well as agarose gels. The separation is determined by the molecular sizes of the analytes as the gel acts as the molecular sieve.

Gel Filtration Chromatography – Principle, Components, Steps, Types, Application

Published on June 26, 2023 by MN Editors

Biomolecules are isolated using various techniques that allow them to be separated by the difference in their unique characteristics like size, hydrophobicity and biorecognition and charge, for example. Gelfiltration is a process where the separation of the components is determined by the the molecular weight, or in size. It is the most simple and gentle of all chromatography techniques and is able to separate molecules based on variations in size.

Gas Chromatography – Definition, Parts, Principle, Working, uses

Published on June 26, 2023 by MN Editors

Gas chromatography Definition, principle, working, uses

The gas chromatography process differs from other types of chromatography because it is gas, and the components are separated into vapours. This allows it to distinguish and identify smaller molecular weight compounds inside the gas phase. The sample is either liquid or gas which is then vaporized at the port for injection. The mobile phase in gas chromatography is a transporter gas, most often helium, because due to its molecular weight being low and its chemical inert. It is pressed, and the mobile phase is able to move the analyte along the column. The separation process is carried out using a column coated stationary phase.

Thin Layer Chromatography – Principle, Components, Procedure, Application

Published on June 26, 2023 by Sourav Bio

constituents of a mix for an analysis that is both qualitative as well as quantitative. For this type of physical separation, the components that are to be separated are divided into two phases. One has a stationary (stationary phase) while the second (the mobile phase) is moving in a specific direction. Based on what phase is stationary and which the mobile phase, they may be of various types.

Classification of Chromatography

Last Updated on February 5, 2022 by MN Editors

Types of Chromatography - Definition, Principle, Steps, Uses

Chromatography is a vital biophysical procedure that permits separate, identification and purification of a mix for both quantitative and qualitative analysis.

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