The Hippurate Hydrolysis Test measures an organism’s ability to create hippuricase, an enzyme that hydrolyzes the substrate hippurate. It identifies Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis. The final products of hippuric acid hydrolysis by hippuricase are glycine and benzoic acid. The oxidising agent ninhydrin deaminates glycine and is reduced in the process. … Read more