Rothera’s Test
MN Editors
Biochemical Test

Rothera’s test Principle, Procedure, Result.

Acetoacetic acid and acetone react with an alkaline solution of sodium nitroprusside to form a purple-colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected.

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