Native Polyacrylamide Gel Electrophoresis (PAGE)
Electrophoresis with agarose or other polyacrylamide gels is an established method to separate the biopolymers, determine their identity and purify them because both of these gels have a porous the natural world. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.