procedure Archives – Microbiology Note

procedure

Triple Beam Balance - Parts, Procedure, Uses

Triple Beam Balance – Parts, Procedure, Uses

Triple Beam Balance Definition The triple beam balance is a tool for precisely measuring mass. Typically, such instruments have a reading inaccuracy of 0.05 grammes. Its name alludes to...

MN Editors in Instruments Sep 29 · 5 min read

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in Biochemical Test

Litmus Milk Test Principle, Procedure, Result

What is the Litmus Milk Test? Litmus Milk Medium is a liquid medium suggested for use in qualitative tests for determining the activity of bacteria on milk and maintaining lactic acid bacteria. The Litmus Milk Test requires a litmus milk medium, which is a liquid medium that differentiates bacteria based on numerous metabolic events in

Sep 10 · 5 min read

Litmus Milk Test Principle, Procedure, Result

in Biochemical Test

Acetamide utilization Test Principle, Procedure, result

Test Name Acetamide utilization Test Purpose Differentiate microorganisms according to their capacity to utilise acetamide as their only carbon source. Microorganisms Tested Isolated colonies of Gram-negative, non-glucose-fermenting rods that emit a luminous pigment and are suggestive of P. aeruginosa. Result Positive: The slant shows growth, and its colour changes from green to intense blue.Negative: There

Sep 9 · 4 min read

Acetamide utilization Test Principle, Procedure, result

in Biochemical Test

Cetrimide Test Principle, Purpose, Procedure, Results

Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.

Sep 8 · 6 min read

Cetrimide Test Principle, Purpose, Procedure, Results

in Biochemical Test

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).

Sep 6 · 2 min read

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

in Biochemical Test

Lipase Test Principle, Procedure, results

The pancreas secretes an enzyme called lipase that aids in the digestion of fatty foods. High levels of lipase in the blood may indicate pancreatitis, a disorder characterised by the inflammation and enlargement of the pancreas. Other pancreatic difficulties, certain drugs, and diseases and disorders include kidney failure, cancer, and gallbladder and oesophagus troubles can

Sep 6 · 4 min read

Lipase Test Principle, Procedure, results

in Laboratory Tests

Widal Test Principle, Procedure, Result, Advantages, Limitation

The Widal test is an agglutination test that determines any antibodies present in the patient's serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).

Nov 30 · 5 min read

Widal Test Principle, Procedure, Result, Advantages, Limitation

in Biochemical Test

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Sep 14 · 2 min read

Tollens’ Test Definition, Principle, Procedure, Result, Application.

in Biochemical Test

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Sep 12 · 6 min read

Oxidase Test Definition, Principle, Procedure, Result, Application

in Microbiology Techniques

Coagulase Test Principle, Procedure, Application, Result.

It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).

Aug 19 · 4 min read

Coagulase Test Principle, Procedure, Application, Result.

in Microbiology Techniques

Counter Current Immunoelectrophoresis Principle, Procedure, Result.

Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.

Aug 17 · 4 min read

Counter Current Immunoelectrophoresis

in Microbiology Techniques

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.

Aug 16 · 3 min read

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

in Microbiology Techniques

Immunoelectrophoresis test Principle, Applications, Procedure, Results, Advantages and Disadvantages.

Immunoelectrophoresis is a powerful qualitative technique for the characterization of an antibody.

Aug 14 · 4 min read

Immunoelectrophoresis test Principle, Applications, Procedure Results, Advantages and Disadvantages.

in Biochemical Test

Gerhardt’s test Principle, Procedure, Result.

Gerhardt's test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.

Jul 19 · 1 min read

Gerhardt's test

in Hematology

Cross Match Principle, Procedure, Result.

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Jul 5 · 4 min read

Cross Match

in Protocols

Reverse Blood Grouping Principle, Procedure, Result

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

Jul 3 · 2 min read

Reverse Blood Grouping

in Protocols

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Jul 3 · 4 min read

Blood Grouping Principle and Procedure

in Biochemical Test

Reverse CAMP test Principle, Procedure, Result

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Jun 22 · 1 min read

Reverse CAMP test

in Biochemical Test

CAMP Test Principle, Procedure, Result

CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.

Jun 22 · 4 min read

Christie, Atkins, and Munch-Peterson (CAMP) test

in Protocols

Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

May 20 · 5 min read

Restriction Digest Principle, Procedure, result

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