procedure Archives – Microbiology Note

procedure

Widal Test Principle, Procedure, Result, Advantages, Limitation

The Widal test is an agglutination test that determines any antibodies present in the patient's serum that are generated against the causal agents of the enteric febrile illness (Salmonella...

MN Editors in Laboratory Tests Nov 30 · 5 min read

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in Biochemical Test

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Sep 14 · 2 min read

Tollens’ Test Definition, Principle, Procedure, Result, Application.

in Biochemical Test

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Sep 12 · 6 min read

Oxidase Test Definition, Principle, Procedure, Result, Application

in Microbiology Techniques

Coagulase Test Principle, Procedure, Application, Result.

It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).

Aug 19 · 4 min read

Coagulase Test Principle, Procedure, Application, Result.

in Microbiology Techniques

Counter Current Immunoelectrophoresis Principle, Procedure, Result.

Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.

Aug 17 · 4 min read

in Microbiology Techniques

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.

Aug 16 · 3 min read

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

in Microbiology Techniques

Immunoelectrophoresis test Principle, Applications, Procedure, Results, Advantages and Disadvantages.

Immunoelectrophoresis is a powerful qualitative technique for the characterization of an antibody.

Aug 14 · 4 min read

Immunoelectrophoresis test Principle, Applications, Procedure Results, Advantages and Disadvantages.

in Biochemical Test

Gerhardt’s test Principle, Procedure, Result.

Gerhardt's test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.

Jul 19 · 1 min read

in Hematology

Cross Match Principle, Procedure, Result.

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Jul 5 · 4 min read

in Protocols

Reverse Blood Grouping Principle, Procedure, Result

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

Jul 3 · 2 min read

in Protocols

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Jul 3 · 4 min read

in Biochemical Test

Reverse CAMP test Principle, Procedure, Result

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Jun 22 · 1 min read

in Biochemical Test

CAMP Test Principle, Procedure, Result

CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.

Jun 22 · 4 min read

Christie, Atkins, and Munch-Peterson (CAMP) test

in Protocols

Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

May 20 · 5 min read

Restriction Digest Principle, Procedure, result

in Protocols

Southern Blotting Technique Principle, Procedure, Importance

Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.

May 19 · 6 min read

Southern Blotting Principle, Procedure, Importance

in Microbiology Techniques

Serial Dilution Method Definition, Procedure, Application

What is a serial dilution? – serial dilution definition Hey there, you might be thinking, what is serial dilution? As the term indicates, it is a series of succeeding dilutions that performed to create a less dense or less concentrated solution from a high dense or concentrated solution. Serial dilution involves performing a series of

Apr 22 · 7 min read

in Microbiology Techniques

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed

Apr 16 · 9 min read

Pour Plate Method Principle, Procedure, Objective, Result

in Microbiology Techniques

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate

Apr 16 · 3 min read

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

in Microbiology Techniques

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species. During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed

Apr 15 · 7 min read

Streak Plate Method: Patterns, Procedure, Principle

in Staining

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. Some

Apr 11 · 1 min read

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Pos.	Name	Score	Duration	Points
1	Harleen Paul	77.5 %	1 minutes 19 seconds	33.25
2	Shree0405	56.67 %	1 minutes 42 seconds	28.33
3	Utkarsh Viswas	53.5 %	1 minutes 28 seconds	23
4	Edmund_Addison	50 %	1 minutes 14 seconds	3
5	SALAMBO12	45 %	1 minutes 25 seconds	22.5
6	Kuli Rebecca	30 %	1 minutes 18 seconds	15
7	Sourav	30 %	1 minutes 36 seconds	15
8	Sourav Bio	17.69 %	14 seconds	11.62
9	DONDA JAHNAVI	0 %	0 second	0

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procedure

Widal Test Principle, Procedure, Result, Advantages, Limitation

LATEST

Tollens’ Test Definition, Principle, Procedure, Result, Application.

Oxidase Test Definition, Principle, Procedure, Result, Application

Coagulase Test Principle, Procedure, Application, Result.

Counter Current Immunoelectrophoresis Principle, Procedure, Result.

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Immunoelectrophoresis test Principle, Applications, Procedure, Results, Advantages and Disadvantages.

Gerhardt’s test Principle, Procedure, Result.

Cross Match Principle, Procedure, Result.

Reverse Blood Grouping Principle, Procedure, Result

Blood Grouping Principle and Procedure

Reverse CAMP test Principle, Procedure, Result

CAMP Test Principle, Procedure, Result

Restriction Digest Protocol, Principle, result

Southern Blotting Technique Principle, Procedure, Importance

Serial Dilution Method Definition, Procedure, Application

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Streak Plate Method: Patterns, Procedure, Principle

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

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Reverse CAMP test Principle, Procedure, Result

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