Pulsed Field Gel Electrophoresis (PFGE) is a method used to aid in the separation of huge deoxyribonucleic acids (DNA) molecules using the gel matrix an electric field that continuously shifts direction. Because DNA greater than 15-20kb moving through a gel is able to move in a size-independent manner.  The conventional gel electrophoresis method was not able to separate massive DNA molecules effectively that led to the use of electrophoresis using pulsed fields.